Quantitative determination of drugs for therapy of COVID-19 in serum and plasma using liquid chromatography ― mass-spectrometry

2020 ◽  
pp. 43-47
Author(s):  
D. A. Farmakovsky
Keyword(s):  
Mass Spectrometry ◽  
Clinical Trials ◽  
Liquid Chromatography ◽  
High Accuracy ◽  
Therapeutic Drug ◽  
Liquid Chromatography Mass Spectrometry ◽  
Accuracy And Precision ◽  
New Drug Development ◽  
Real Clinical Practice

There was developed a method for quantitation of the drugs which could be effective for COVID-19 treatment. Using tandem liquid mass-spectrometry it is possible to simultaneously quantify with high accuracy and precision up to 9 medicines in human plasma. This method could be used for new drug development as well as for clinical trials and therapeutic drug monitoring in real clinical practice.

scholarly journals Simultaneous Determination of Levocetirizine and Pseudoephedrine in Dog Plasma by Liquid Chromatography-Mass Spectrometry in the Presence of Dextrocetirizine

2012 ◽  
Vol 15 (4) ◽  
pp. 519 ◽  
Author(s):  
Jae Kuk Ryu ◽  
Sun Dong Yoo
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Oral Administration ◽  
Mobile Phase ◽  
Internal Standard ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chiral Column ◽  
Accuracy And Precision ◽  
Dog Plasma

Purpose. This study describes the development of a rapid and sensitive LC-ESI-MS assay for simultaneous enantioselective determination of levocetirizine and pseudoephedrine in dog plasma in the presence of dextrocetirizine. Methods. Separations were achieved on an Ultron ES-OVM chiral column using the mobile phase consisting of 10 mM aqueous NH4OAc (pH 6.6) and acetonitrile (9:1 v/v). Results. The retention times of pseudoephedrine, dextrocetirizine, levocetirizine and diazepam (internal standard) were 5.2, 8.3, 9.6 and 11.6 min, respectively, and the total run time was less than 15 min. The assay was validated to demonstrate the linearity, accuracy and precision, recovery and stability. The calibration curves were linear over the concentration range from 1 – 200 ng/mL for levocetirizine and from 5 – 1000 ng/mL for pseudoephedrine. Conclusions. The developed assay was successfully applied to a pharmacokinetic study after oral administration of the racemic cetirizine (0.5 mg/kg, or 0.25 mg/kg as levocetirizine) and pseudoephedrine (12 mg/kg) in the dog. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

scholarly journals Determination of regulatory ionophore coccidiostat residues in feedstuffs at carry-over levels by liquid chromatography-mass spectrometry

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0182831 ◽  
Author(s):  
Loredana Annunziata ◽  
Pierina Visciano ◽  
Arianna Stramenga ◽  
Maria Novella Colagrande ◽  
Guido Campana ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Carry Over
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