Practical Manual for Plant Disease Assessment

Author(s):  
Imran Ul Haq ◽  
Amer Habib
1998 ◽  
Vol 88 (5) ◽  
pp. 446-449 ◽  
Author(s):  
C. Raikes ◽  
L. L. Burpee

The ability to identify diseases early and quantify severity accurately is crucial in plant disease assessment and management. This study was conducted to assess changes in the spectral reflectance of sunlight from plots of creeping bentgrass during infection by Rhizoctonia solani, the cause of Rhizoctonia blight, and to evaluate multispectral radiometry as a tool to quantify Rhizoctonia blight severity. After inoculation of 6-year-old creeping bentgrass turf with R. solani anastomosis group 2-2, reflectance of sunlight from the foliar canopy was measured at light wavelengths of 460 nm (blue) to 810 nm (near infrared [NIR]), at 50-nm intervals. Visual estimates of disease severity and percentage of canopy reflectance were made daily throughout each of three epidemics of Rhizoctonia blight from the onset of visible symptoms until maximum disease severity was reached. In each experiment, linear regression analysis revealed a significant reduction in the percentage of NIR (760 and 810 nm) reflectance as disease severity increased. However, in the majority of analyses, regression models explained <50% of the variability between components. Multispectrum radiometry appears to function best when used to assess differences in disease severity at discrete points in time rather than over an entire epidemic.


Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 565 ◽  
Author(s):  
Hung I. Liu ◽  
Jia Ren Tsai ◽  
Wen Hsin Chung ◽  
Clive H. Bock ◽  
Kuo Szu Chiang

Estimates of plant disease severity are crucial to various practical and research-related needs in agriculture. Ordinal scales are used for categorizing severity into ordered classes. Certain characteristics of quantitative ordinal scale design may affect the accuracy of the specimen estimates and, consequently, affect the accuracy of the resulting mean disease severity for the sample. The aim of this study was to compare mean estimates based on various quantitative ordinal scale designs to the nearest percent estimates, and to investigate the effect of the number of classes in an ordinal scale on the accuracy of that mean. A simulation method was employed. The criterion for comparison was the mean squared error of the mean disease severity for each of the different scale designs used. The results indicate that scales with seven or more classes are preferable when actual mean disease severities of 50% or less are involved. Moreover, use of an amended 10% quantitative ordinal scale with additional classes at low severities resulted in a more accurate mean severity compared to most other scale designs at most mean disease severities. To further verify the simulation results, estimates of mean severity of pear scab on samples of leaves from orchards in Taiwan demonstrated similar results. These observations contribute to the development of plant disease assessment scales to improve the accuracy of estimates of mean disease severities.


Plant Disease ◽  
2016 ◽  
Vol 100 (2) ◽  
pp. 241-251 ◽  
Author(s):  
Anne-Katrin Mahlein

Early and accurate detection and diagnosis of plant diseases are key factors in plant production and the reduction of both qualitative and quantitative losses in crop yield. Optical techniques, such as RGB imaging, multi- and hyperspectral sensors, thermography, or chlorophyll fluorescence, have proven their potential in automated, objective, and reproducible detection systems for the identification and quantification of plant diseases at early time points in epidemics. Recently, 3D scanning has also been added as an optical analysis that supplies additional information on crop plant vitality. Different platforms from proximal to remote sensing are available for multiscale monitoring of single crop organs or entire fields. Accurate and reliable detection of diseases is facilitated by highly sophisticated and innovative methods of data analysis that lead to new insights derived from sensor data for complex plant-pathogen systems. Nondestructive, sensor-based methods support and expand upon visual and/or molecular approaches to plant disease assessment. The most relevant areas of application of sensor-based analyses are precision agriculture and plant phenotyping.


2019 ◽  
Vol 11 (21) ◽  
pp. 2495 ◽  
Author(s):  
David Bohnenkamp ◽  
Jan Behmann ◽  
Anne-Katrin Mahlein

The application of hyperspectral imaging technology for plant disease detection in the field is still challenging. Existing equipment and analysis algorithms are adapted to highly controlled environmental conditions in the laboratory. However, only real time information from the field scale is able to guide plant protection measures and to optimize the use of resources. At the field scale, many parameters such as the optimal measurement distance, informative feature sets, and suitable algorithms have not been investigated. In this study, the hyperspectral detection and quantification of yellow rust in wheat was evaluated using two measurement platforms: a ground-based vehicle and an unmanned aerial vehicle (UAV). Different disease development stages and disease severities were provided in a plot-based field experiment. Measurements were performed weekly during the vegetation period. Data analysis was performed by three prediction algorithms with a focus on the selection of optimal feature sets. In this context, the across-scale application of optimized feature sets, an approach of information transfer between scales, was also evaluated. Relevant aspects for an on-line disease assessment in the field integrating affordable sensor technology, sensor spatial resolution, compact analysis models, and fast evaluation have been outlined and reflected upon. For the first time, a hyperspectral imaging observation experiment of a plant disease was comparatively performed at two scales, ground canopy and UAV.


2017 ◽  
Vol 107 (10) ◽  
pp. 1092-1094 ◽  
Author(s):  
P. S. Ojiambo ◽  
J. Yuen ◽  
F. van den Bosch ◽  
L. V. Madden

Epidemiology has made significant contributions to plant pathology by elucidating the general principles underlying the development of disease epidemics. This has resulted in a greatly improved theoretical and empirical understanding of the dynamics of disease epidemics in time and space, predictions of disease outbreaks or the need for disease control in real-time basis, and tactical and strategic solutions to disease problems. Availability of high-resolution experimental data at multiple temporal and spatial scales has now provided a platform to test and validate theories on the spread of diseases at a wide range of spatial scales ranging from the local to the landscape level. Relatively new approaches in plant disease epidemiology, ranging from network to information theory, coupled with the availability of large-scale datasets and the rapid development of computer technology, are leading to revolutionary thinking about epidemics that can result in considerable improvement of strategic and tactical decision making in the control and management of plant diseases. Methods that were previously restricted to topics such as population biology or evolution are now being employed in epidemiology to enable a better understanding of the forces that drive the development of plant disease epidemics in space and time. This Focus Issue of Phytopathology features research articles that address broad themes in epidemiology including social and political consequences of disease epidemics, decision theory and support, pathogen dispersal and disease spread, disease assessment and pathogen biology and disease resistance. It is important to emphasize that these articles are just a sample of the types of research projects that are relevant to epidemiology. Below, we provide a succinct summary of the articles that are published in this Focus Issue .


Author(s):  
Karen K. Baker ◽  
David L. Roberts

Plant disease diagnosis is most often accomplished by examination of symptoms and observation or isolation of causal organisms. Occasionally, diseases of unknown etiology occur and are difficult or impossible to accurately diagnose by the usual means. In 1980, such a disease was observed on Agrostis palustris Huds. c.v. Toronto (creeping bentgrass) putting greens at the Butler National Golf Course in Oak Brook, IL.The wilting symptoms of the disease and the irregular nature of its spread through affected areas suggested that an infectious agent was involved. However, normal isolation procedures did not yield any organism known to infect turf grass. TEM was employed in order to aid in the possible diagnosis of the disease.Crown, root and leaf tissue of both infected and symptomless plants were fixed in cold 5% glutaraldehyde in 0.1 M phosphate buffer, post-fixed in buffered 1% osmium tetroxide, dehydrated in ethanol and embedded in a 1:1 mixture of Spurrs and epon-araldite epoxy resins.


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