The Effect of Fibroblast Growth Factor 2 on the In Vitro Expression of Syndecan-4 and Glypican-1 in Turkey Satellite Cells

AbstractFibroblast growth factor 2 (FGF2) augments podocyte injury, which induces glomerulosclerosis, although the mechanisms remain obscure. In this study, we investigated the effects of FGF2 on cultured podocytes with interdigitating cell processes in rats. After 48 h incubation with FGF2 dynamic changes in the shape of primary processes and cell bodies of podocytes resulted in the loss of interdigitation, which was clearly shown by time-lapse photography. FGF2 reduced the gene expressions of constituents of the slit diaphragm, inflections of intercellular junctions positive for nephrin, and the width of the intercellular space. Immunostaining for the proliferation marker Ki-67 was rarely seen and weakly stained in the control without FGF2, whereas intensely stained cells were frequently found in the presence of FGF2. Binucleation and cell division were also observed, although no significant increase in cell number was shown. An in vitro scratch assay revealed that FGF2 enhanced migration of podocytes. These findings show that FGF2 makes podocytes to transition from the quiescent state into the cell cycle and change their morphology due to enhanced motility, and that the culture system in this study is useful for analyzing the pathological changes of podocytes in vivo.

Download Full-text

Human vascular injury triggers fibroblast growth factor 2 (FGF 2) release. Neointima formation in vitro is inhibited by novel catalytic DNA molecules targeting human EGR-1

Heart Lung and Circulation ◽

10.1046/j.1443-9506.2000.0622x.x ◽

2000 ◽

Vol 9 (3) ◽

pp. A95

Author(s):

H.C. Lowe ◽

M. Costandi ◽

C.N. Chesterman ◽

L.M. Khachigian

Keyword(s):

Growth Factor ◽

Fibroblast Growth Factor ◽

Vascular Injury ◽

Fibroblast Growth Factor 2 ◽

Neointima Formation ◽

Fibroblast Growth ◽

Dna Molecules ◽

Catalytic Dna

Download Full-text

S100ß and fibroblast growth factor-2 are present in cultured Schwann cells and may exert paracrine actions on the peripheral nerve injury

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502008000600014 ◽

2008 ◽

Vol 23 (6) ◽

pp. 555-560 ◽

Cited By ~ 11

Author(s):

Tatiana Duobles ◽

Thais de Sousa Lima ◽

Beatriz de Freitas Azevedo Levy ◽

Gerson Chadi

Keyword(s):

Growth Factor ◽

Sciatic Nerve ◽

Fibroblast Growth Factor ◽

Schwann Cells ◽

Nerve Injury ◽

Satellite Cells ◽

Peripheral Nerves ◽

Fibroblast Growth Factor 2 ◽

Fibroblast Growth ◽

Cultured Schwann Cells

PURPOSE: The neurotrophic factor fibroblast growth factor-2 (FGF-2, bFGF) and Ca++ binding protein S100ß are expressed by the Schwann cells of the peripheral nerves and by the satellite cells of the dorsal root ganglia (DRG). Recent studies have pointed out the importance of the molecules in the paracrine mechanisms related to neuronal maintenance and plasticity of lesioned motor and sensory peripheral neurons. Moreover, cultured Schwann cells have been employed experimentally in the treatment of central nervous system lesions, in special the spinal cord injury, a procedure that triggers an enhanced sensorymotor function. Those cells have been proposed to repair long gap nerve injury. METHODS: Here we used double labeling immunohistochemistry and Western blot to better characterize in vitro and in vivo the presence of the proteins in the Schwann cells and in the satellite cells of the DRG as well as their regulation in those cells after a crush of the rat sciatic nerve. RESULTS: FGF-2 and S100ß are present in the Schwann cells of the sciatic nerve and in the satellite cells of the DRG. S100ß positive satellite cells showed increased size of the axotomized DRG and possessed elevated amount of FGF-2 immunoreactivity. Reactive satellite cells with increased FGF-2 labeling formed a ring-like structure surrounding DRG neuronal cell bodies.Reactive S100ß positive Schwann cells of proximal stump of axotomized sciatic nerve also expressed higher amounts of FGF-2. CONCLUSION: Reactive peripheral glial cells synthesizing FGF-2 and S100ß may be important in wound repair and restorative events in the lesioned peripheral nerves.

Download Full-text

A New 34-Kilodalton Isoform of Human Fibroblast Growth Factor 2 Is Cap Dependently Synthesized by Using a Non-AUG Start Codon and Behaves as a Survival Factor

Molecular and Cellular Biology ◽

10.1128/mcb.19.1.505 ◽

1999 ◽

Vol 19 (1) ◽

pp. 505-514 ◽

Cited By ~ 146

Author(s):

Emmanuelle Arnaud ◽

Christian Touriol ◽

Christel Boutonnet ◽

Marie-Claire Gensac ◽

Stéphan Vagner ◽

...

Keyword(s):

Growth Factor ◽

Fibroblast Growth Factor ◽

Human Fibroblast ◽

The Other ◽

In Vitro Translation ◽

Start Codon ◽

Fibroblast Growth Factor 2 ◽

Initiation Codon ◽

Fibroblast Growth

ABSTRACT Four isoforms of human fibroblast growth factor 2 (FGF-2) result from alternative initiations of translation at three CUG start codons and one AUG start codon. Here we characterize a new 34-kDa FGF-2 isoform whose expression is initiated at a fifth initiation codon. This 34-kDa FGF-2 was identified in HeLa cells by using an N-terminal directed antibody. Its initiation codon was identified by site-directed mutagenesis as being a CUG codon located at 86 nucleotides (nt) from the FGF-2 mRNA 5′ end. Both in vitro translation and COS-7 cell transfection using bicistronic RNAs demonstrated that the 34-kDa FGF-2 was exclusively expressed in a cap-dependent manner. This contrasted with the expression of the other FGF-2 isoforms of 18, 22, 22.5, and 24 kDa, which is controlled by an internal ribosome entry site (IRES). Strikingly, expression of the other FGF-2 isoforms became partly cap dependent in vitro in the presence of the 5,823-nt-long 3′ untranslated region of FGF-2 mRNA. Thus, the FGF-2 mRNA can be translated both by cap-dependent and IRES-driven mechanisms, the balance between these two mechanisms modulating the ratio of the different FGF-2 isoforms. The function of the new FGF-2 was also investigated. We found that the 34-kDa FGF-2, in contrast to the other isoforms, permitted NIH 3T3 cell survival in low-serum conditions. A new arginine-rich nuclear localization sequence (NLS) in the N-terminal region of the 34-kDa FGF-2 was characterized and found to be similar to the NLS of human immunodeficiency virus type 1 Rev protein. These data suggest that the function of the 34-kDa FGF-2 is mediated by nuclear targets.

Download Full-text

Localized delivery of fibroblast growth factor–2 and brain-derived neurotrophic factor reduces spontaneous seizures in an epilepsy model

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.0810710106 ◽

2009 ◽

Vol 106 (17) ◽

pp. 7191-7196 ◽

Cited By ~ 96

Author(s):

Beatrice Paradiso ◽

Peggy Marconi ◽

Silvia Zucchini ◽

Elena Berto ◽

Anna Binaschi ◽

...

Keyword(s):

Growth Factor ◽

Fibroblast Growth Factor ◽

Neurotrophic Factor ◽

Viral Vectors ◽

Neuronal Damage ◽

Brain Derived Neurotrophic Factor ◽

Fibroblast Growth Factor 2 ◽

Fibroblast Growth ◽

Damage Limitation

A loss of neurons is observed in the hippocampus of many patients with epilepsies of temporal lobe origin. It has been hypothesized that damage limitation or repair, for example using neurotrophic factors (NTFs), may prevent the transformation of a normal tissue into epileptic (epileptogenesis). Here, we used viral vectors to locally supplement two NTFs, fibroblast growth factor–2 (FGF-2) and brain-derived neurotrophic factor (BDNF), when epileptogenic damage was already in place. These vectors were first characterized in vitro, where they increased proliferation of neural progenitors and favored their differentiation into neurons, and they were then tested in a model of status epilepticus-induced neurodegeneration and epileptogenesis. When injected in a lesioned hippocampus, FGF-2/BDNF expressing vectors increased neuronogenesis, embanked neuronal damage, and reduced epileptogenesis. It is concluded that reduction of damage reduces epileptogenesis and that supplementing specific NTFs in lesion areas represents a new approach to the therapy of neuronal damage and of its consequences.

Download Full-text