The prevalence and genetic characterization of Chlamydia psittaci from domestic and feral pigeons in Poland and the correlation between infection rate and incidence of pigeon circovirus

AbstractCoagulase-positive staphylococci (CoPS) are opportunistic veterinary pathogens, of whichStaphylococcus aureus,S. delphiniandS. intermediuscan be isolated from pigeons. The biochemical identification ofS. delphiniandS. intermediusisolates may be incorrect, because of their phenotypic similarity. The purpose of the present study was to isolate and identify CoPS from domestic and feral pigeons and to determine their genetic relatedness by PFGE. A total number of 31 isolates of CoPS were obtained, 15 were identified asS. delphinigroup B, six asS. aureus,four asS. delphinigroup A, three asS. intermediusand three asS. schleiferisubsp.coagulans. The results indicate that S.delphinigroup B is the predominant CoPS species among pigeons studied. PFGE restriction patterns ofS. delphinigroup A andS. delphinigroup B form separate clusters, demonstrating their genetic heterogeneity. Indistinguishable or very similar PFGE patterns observed amongS. delphinigroup B isolates from domestic and feral pigeons confirm the possibility of CoPS transmission between these birds.

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Cryptosporidium in asymptomatic children in Southern Xinjiang, China and the potential of zoonotic transmission

10.21203/rs.3.rs-551246/v1 ◽

2021 ◽

Author(s):

Tian Wang ◽

Zilin Wei ◽

Ying Zhang ◽

Qiyuan Zhang ◽

Longxian Zhang ◽

...

Keyword(s):

Infection Rate ◽

Maximum Likelihood Method ◽

Genetic Characterization ◽

Pcr Amplification ◽

Likelihood Method ◽

Limited Information ◽

Genetic Characteristics ◽

Asymptomatic Children ◽

Southern Xinjiang

Abstract Background: Cryptosporidium is a primary cause of diarrhea in children globally. However, there is limited information on the prevalence and genetic characterization of Cryptosporidium in children in Xinjiang, China. This study aimed to assess the genetic characteristics and epidemiological status of Cryptosporidium in asymptomatic children in Southern Xinjiang, China.Methods: A total of 609 fecal samples were collected from kindergartners aged 2-6 y from 11 counties of Southern Xinjiang, China. We used nested PCR amplification of partial SSU rDNA gene to screen the samples for Cryptosporidium spp. The isolates containing C. parvum and C. hominis were further subtyped by a 60-kDa glycoprotein (gp60). We used MEGA7 to construct a phylogenetic tree to study the genetic relationship between the gp60 subtypes of these two species via the Maximum Likelihood method based on the Tamura-Nei model.Results: Only 1.3% (8/609) of asymptomatic children were confirmed as infected with Cryptosporidium with 2.0% (6/299) infection rate in boys and 0.6% (2/310) infection rate in girls. Three Cryptosporidium species were identified including C. felis (37.5%; 3/8), C. hominis (37.5%; 3/8) and C. parvum (25.0%; 2/8). Three C. hominis subtypes (IbA9G3, IdA14 and IfA12G1) and two C. parvum subtypes (IIdA14G1 and IIdA15G1) were also found.Conclusions: This study was the first to identify the presence of cryptosporidium in asymptomatic children in Southern Xinjiang, China. The presence of zoonotic C. parvum subtypes IIdA14G1 and IIdA15G1 indicates possible crossspecies transmission of Cryptosporidium between children and animals.

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Histopathological and genetic characterization of colorectal mucinous carcinoma

Gastroenterology ◽

10.1016/s0016-5085(01)80821-0 ◽

2001 ◽

Vol 120 (5) ◽

pp. A166-A166

Author(s):

S FUJII ◽

T KUSAKA ◽

T KAIHARA ◽

Y UEDA ◽

T CHIBA ◽

...

Keyword(s):

Genetic Characterization ◽

Mucinous Carcinoma

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Genetic characterization of high hyperdiploidy in childhood acute lymphoblastic leukemia

Klinische Pädiatrie ◽

10.1055/s-0029-1222694 ◽

2009 ◽

Vol 221 (03) ◽

Author(s):

R Vagkopoulou ◽

C Eckert ◽

U Ungethüm ◽

G Körner ◽

M Stanulla ◽

...

Keyword(s):

Acute Lymphoblastic Leukemia ◽

Genetic Characterization ◽

Lymphoblastic Leukemia ◽

Childhood Acute Lymphoblastic Leukemia

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TBE in Sweden

Tick-borne encephalitis - The Book ◽

10.33442/26613980_12b32-3 ◽

2020 ◽

Keyword(s):

Genetic Characterization ◽

Immunoglobulin M ◽

Enzyme Linked Immunosorbent Assay ◽

Second Phase ◽

Serum Samples ◽

Tick Borne Encephalitis ◽

Specific Immunoglobulin ◽

Tick Borne Encephalitis Virus ◽

First Time

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.

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