scholarly journals MALDI-TOF mass spectrometry in clinical diagnostic microbiology

JMS SKIMS ◽  
2017 ◽  
Vol 20 (1) ◽  
pp. 54
Author(s):  
Editor SKIMS
Keyword(s):  
Mass Spectrometry ◽  
Low Cost ◽  
Microbial Identification ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Clinical Diagnostic ◽  
Routine Identification ◽  
Identification Technique ◽  
Diagnostic Microbiology ◽  
Identification Techniques

Microbial identification in clinical diagnostic laboratories mainly relies on conventional phenotypic and gene sequencing identification techniques. Recently development of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) devices has revolutionized the routine identification of microorganisms in clinical microbiology laboratories. This is an easy, rapid, high throughput, low-cost, and efficient identification technique and has been adapted to the constraint of clinical diagnostic laboratories. This technology has the potential to replace and/or complement conventional identification techniques for both bacterial and fungal strains. JMS 2017; 20(1):54

scholarly journals Rapid microbial identifications by MALDI-TOF – Viva la revolution!

2010 ◽  
Vol 31 (3) ◽  
pp. 111
Author(s):  
Stephen A Neville ◽  
Iain B Gosbell
Keyword(s):  
Mass Spectrometry ◽  
Bacterial Identification ◽  
Maldi Tof Ms ◽  
Microbial Identification ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Identification Of Species ◽  
Phenotypic Methods ◽  
Diagnostic Microbiology ◽  
Tof Ms

Although the application of mass spectrometry to bacterial identification was proposed as far back as 1975 by John Anhalt, it was not until 1987 when Professor Franz Hillenkamp and Dr Michael Karas first pioneered Matrix Assisted Laser Desorption/Ionisation Time-of-Flight mass spectrometry (MALDI-TOF MS) for truly rapid microbial identification in diagnostic microbiology laboratories. Conventional phenotypic methods of identifying isolates of bacteria, yeasts and filamentous fungi require hours to several days to complete, depending on the type of organism involved, and may be prone to error due to bias or inexperience. Even the more recent molecular innovations have their limitations that place them outside the scope and budget of many routine laboratories. However, MALDI-TOF MS technology has changed the way we think about microbial identifications and strain differentiation by providing results from plate to name in approximately five minutes for one isolate and around 90 minutes for 60 isolates at under $2 an identification. It is likely to be particularly helpful clinically for identification of species in positive blood culture broths and for organisms that are hard to identify quickly.

scholarly journals Application of MALDI-TOF mass spectrometry in clinical diagnostic microbiology

2014 ◽  
Vol 8 (09) ◽  
pp. 1081-1088 ◽  
Author(s):  
Elena De Carolis ◽  
Antonietta Vella ◽  
Luisa Vaccaro ◽  
Riccardo Torelli ◽  
Teresa Spanu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drug Susceptibility ◽  
Biochemical Tests ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Bacteria And Fungi ◽  
Diagnostic Microbiology ◽  
Tof Ms

Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently emerged as a powerful technique for identification of microorganisms, changing the workflow of well-established laboratories so that its impact on microbiological diagnostics has been unparalleled. In comparison with conventional identification methods that rely on biochemical tests and require long incubation procedures, MALDI-TOF MS has the advantage of identifying bacteria and fungi directly from colonies grown on culture plates in a few minutes and with simple procedures. Numerous studies on different systems available demonstrate the reliability and accuracy of the method, and new frontiers have been explored besides microbial species level identification, such as direct identification of pathogens from positive blood cultures, subtyping, and drug susceptibility detection.

scholarly journals A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification

2019 ◽  
Vol 5 (1) ◽  
pp. 4 ◽  
Author(s):  
Robin Patel
Keyword(s):  
Mass Spectrometry ◽  
Filamentous Fungi ◽  
Bacterial Identification ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Fungal Identification ◽  
Routine Identification ◽  
Tof Ms

As a result of its being inexpensive, easy to perform, fast and accurate, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) is quickly becoming the standard means of bacterial identification from cultures in clinical microbiology laboratories. Its adoption for routine identification of yeasts and even dimorphic and filamentous fungi in cultures, while slower, is now being realized, with many of the same benefits as have been recognized on the bacterial side. In this review, the use of MALDI-ToF MS for identification of yeasts, and dimorphic and filamentous fungi grown in culture will be reviewed, with strengths and limitations addressed.

scholarly journals Intact Staphylococcus Enterotoxin SEB from Culture Supernatant Detected by MALDI-TOF Mass Spectrometry

Toxins ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 101 ◽  
Author(s):  
Jenna Tonacini ◽  
Dario Stephan ◽  
Guido Vogel ◽  
Marc-André Avondet ◽  
Franka Kalman ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Culture Supernatant ◽  
Main Peak ◽  
Amino Acid Sequence Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Routine Identification ◽  
Intracellular Proteins ◽  
Tof Ms

Routine identification of pathogens by MALDI-TOF MS (matrix-assisted laser desorption ionisation time-of-flight mass spectrometry) is based on the fingerprint of intracellular proteins. This work evaluated the use of MALDI-TOF MS for the identification of extracellular pathogen factors. A Staphylococcus aureus isolate from a food contaminant was exponentially grown in liquid cultures. Secreted proteins were collected using methanol– chloroform precipitation and analysed by MALDI-TOF MS. A main peak m/z 28,250 was demonstrated, which was identified as S.aureus enterotoxin type B (SEB) by using the pure authentic SEB reference of 28.2 kDa and by amino acid sequence analysis. SEB was also detected in this intact form following pasteurization and cooking treatments. Further application of the elaborated MALDI-TOF MS protocol resulted in the detection of SEA at m/z 27,032 and SEC at m/z 27,629. In conclusion, a simple sample preparation from S.aureus cultures and an easy-to-perform identification of pathogen factors SE in intact form represents a promising next-generation application of MALDI-TOF MS.

scholarly journals Rapid identification of Nocardia cyriacigeorgica from a brain abscess patient using MALDI-TOF-MS

2020 ◽  
Vol 2020 (10) ◽  
Author(s):  
Mohammed AlMogbel ◽  
Mohammed AlBolbol ◽  
Noura Elkhizzi ◽  
Hisham AlAjlan ◽  
John Philip Hays ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Bacterial Species ◽  
Rapid Identification ◽  
Clinical Samples ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Nocardia Cyriacigeorgica ◽  
Diagnostic Microbiology ◽  
Tof Ms

Abstract Nocardia cyriacigeorgica (N. cyriacigeorgica) is most frequently associated with human infections, including chronic bronchitis, pulmonary disease and brain abscesses. In general, N. cyriacigeorgica causes infections in immunocompromised individuals and has been reported in clinical samples worldwide. However, the isolation and speciation of N. cyriacigeorgica in the routine diagnostic microbiology laboratory are complicated and time consuming. Recent mass spectrometry techniques such as matrix-assisted laser desorption/ionization time-of-flight-mass spectrometry (MALDI-TOF-MS) have been successfully integrated into many routine diagnostic microbiology laboratories, allowing for the rapid, accurate and simple identification and speciation of many different microorganisms, including difficult-to-identify bacterial species. Here, we present a case report of a 65-year-old female patient from the neurology ward of Prince Sultan Military Medical City in Riyadh, Saudi Arabia, who was infected with N. cyriacigeorgica. The bacterium was successfully identified by MALDI-TOF-MS, with species identification subsequently confirmed by sequence analysis of the 16S ribosomal RNA.

scholarly journals Construction and Application of MALDI-TOF Mass Spectrometry for the Detection of Haemophilus parasuis

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Xiaoxu Wang ◽  
Feng Xu ◽  
Kun Ning ◽  
Liping Shen ◽  
Xinyong Qi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Clinical Diagnosis ◽  
Culture Media ◽  
The Self ◽  
Haemophilus Parasuis ◽  
Ionization Time ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Fingerprint Database ◽  
Protein Fingerprint

To construct a protein fingerprint database of Haemophilus parasuis (H. parasuis), thus improving its clinical diagnosis efficiency. A total of 15 H. parasuis standard strains were collected to establish a protein fingerprint database of H. parasuis using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and the effects of different culture media and culture time on the quality and identification results of the protein fingerprint were investigated. The results showed that tryptone soy agar (TSA) and tryptone soy broth (TSB) media and different incubation times had no significant effect on the characteristic peaks of the protein profiles. In addition, 18 clinical isolates were used to compare the identification results of the self-built protein fingerprint database, PCR detection, and basic database. Only one strain was identified in the original VITEK-MS system database, while the self-made protein fingerprint database of H. parasuis was 100% accurate for the detection of 18 clinical isolate strains. The protein fingerprint database of H. parasuis built by our laboratory is suitable for rapid clinical diagnosis of H. parasuis, due to its high accuracy, efficiency, and strong specificity.

Sign in / Sign up

Export Citation Format

Share Document