A Novel Approach to Enhance Crude Oil Recovery Ratio Using Selected Bacterial Species

The high viscosity and low flow properties of some crude oil make them difficult to extract from oil reservoirs. This study investigated the mechanisms responsible for the enhancement of oil recovery using fractured dolomite core models. Bacterial strains, Nocardia cyriacigeorgica, Bacillus species, and Pseudomonasputida, isolated from Libyan oil fields, had the ability to biotransform heavy crude oil by reducing its viscosity and converting heavier components into lighter ones. The efficiencies of the three bacterial strains were assessed using sand-packed column experiments through the injection of bacteria to mimic in-situ oil recovery. The optimum biotransformation values of Libyan Bouri crude oil were determined as 77.1, 61.2, and 61.1% using the Bacillus sp., P. putida, and Nocardia cyriacigeorgica, respectively, at 55 °C. Viscosity analyses showed that these strains resulted in the reduction of the viscosity of the crude oil at two different temperatures of 37 and 55 °C. The highest recovery of residual oil was about 11.3% using Bacillus sp. The study confirmed that the selected bacterial species were capable of displacing additional oil under simulated oil field conditions.

Efficient differentiation of Nocardia farcinica, Nocardia cyriacigeorgica and Nocardia beijingensis by high-resolution melting analysis using a novel locus

Accurate identification of Nocardia species remains a challenge due to the complexities of taxonomy and insufficient discriminatory power of traditional techniques. We report the development of a molecular technique that utilizes real-time PCR-based high-resolution melting (HRM) analysis for differentiation of the most common Nocardia species. Based on a novel fusA-tuf intergenic region sequence, Nocardia farcinica , Nocardia cyriacigeorgica and Nocardia beijingensis were clearly distinguished from one another by HRM analysis. The limit of detection of the HRM assay for purified Nocardia spp. DNA was at least 10 fg. No false positives were observed for specificity testing of 20 non-target clinical samples. In comparison to established matrix-assisted laser desorption/ionization-time of flight MS, the HRM assay improved the identification of N. beijingensis . Additionally, all the products of PCR were verified by direct sequencing. In conclusion, the developed molecular assay allows simultaneous detection and differentiation of N. farcinica , N. cyriacigeorgica and N. beijingensis with high sensitivity and specificity.

Disseminated Nocardia infection with a lesion occupying the intracranial space complicated with coma: a case report

Background Disseminated Nocardia infection is a disease that is easily overlooked in patients with lesions occupying the intracranial space complicated with coma. Early diagnosis and treatment are crucial. Case presentation A 65-year-old man was admitted to the First Affiliated Hospital of Zhejiang University in October 2018 with weakness in the right limbs for 3 days and altered consciousness for 1 day. Five months earlier, he had been diagnosed with membranous kidney disease and had received cyclophosphamide and prednisone. At admission, the white blood cell count was 1.37 × 1010/L (with 86.4% neutrophils), and C-reactive protein was 115.60 mg/L. Imaging examinations revealed a lesion occupying the intracranial space, lung infection, and multiple abscesses in the rhomboid muscle. The abscesses were drained. Pus culture confirmed Nocardia cyriacigeorgica infection. With antibiotics and vacuum-sealed drainage of the back wound, the patient improved and was discharged from the hospital. Conclusions This case report shows that infection should be considered during the differential diagnosis of lesions in the intracranial space, especially in patients receiving immunosuppressive treatment. In patients with disseminated N. cyriacigeorgica infection, combination antibiotic therapy and surgical drainage of localised abscesses can be effective.

Post phacoemulsification wound infection by Nocardia cyriacigeorgica: a rare acid-fast organism

Tunnel infection is one of the most dreaded infections, post phacoemulsification. Most cases reported till now are caused by Gram-positive and Gram-negative bacteria or fungi and very few cases by the acid-fast organisms. Ocular infections by Nocardia usually have poor prognosis. Here, we report a case of tunnel infection, its management and microbiological identification of cause behind it, that is, Nocardia cyriacigeorgica a rare acid-fast microorganism.

Rapid identification of Nocardia cyriacigeorgica from a brain abscess patient using MALDI-TOF-MS

Nocardia cyriacigeorgica (N. cyriacigeorgica) is most frequently associated with human infections, including chronic bronchitis, pulmonary disease and brain abscesses. In general, N. cyriacigeorgica causes infections in immunocompromised individuals and has been reported in clinical samples worldwide. However, the isolation and speciation of N. cyriacigeorgica in the routine diagnostic microbiology laboratory are complicated and time consuming. Recent mass spectrometry techniques such as matrix-assisted laser desorption/ionization time-of-flight-mass spectrometry (MALDI-TOF-MS) have been successfully integrated into many routine diagnostic microbiology laboratories, allowing for the rapid, accurate and simple identification and speciation of many different microorganisms, including difficult-to-identify bacterial species. Here, we present a case report of a 65-year-old female patient from the neurology ward of Prince Sultan Military Medical City in Riyadh, Saudi Arabia, who was infected with N. cyriacigeorgica. The bacterium was successfully identified by MALDI-TOF-MS, with species identification subsequently confirmed by sequence analysis of the 16S ribosomal RNA.