scholarly journals Construction and Application of MALDI-TOF Mass Spectrometry for the Detection of Haemophilus parasuis

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Xiaoxu Wang ◽  
Feng Xu ◽  
Kun Ning ◽  
Liping Shen ◽  
Xinyong Qi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Clinical Diagnosis ◽  
Culture Media ◽  
The Self ◽  
Haemophilus Parasuis ◽  
Ionization Time ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Fingerprint Database ◽  
Protein Fingerprint

To construct a protein fingerprint database of Haemophilus parasuis (H. parasuis), thus improving its clinical diagnosis efficiency. A total of 15 H. parasuis standard strains were collected to establish a protein fingerprint database of H. parasuis using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and the effects of different culture media and culture time on the quality and identification results of the protein fingerprint were investigated. The results showed that tryptone soy agar (TSA) and tryptone soy broth (TSB) media and different incubation times had no significant effect on the characteristic peaks of the protein profiles. In addition, 18 clinical isolates were used to compare the identification results of the self-built protein fingerprint database, PCR detection, and basic database. Only one strain was identified in the original VITEK-MS system database, while the self-made protein fingerprint database of H. parasuis was 100% accurate for the detection of 18 clinical isolate strains. The protein fingerprint database of H. parasuis built by our laboratory is suitable for rapid clinical diagnosis of H. parasuis, due to its high accuracy, efficiency, and strong specificity.

scholarly journals Application of MALDI-TOF mass spectrometry in clinical diagnostic microbiology

2014 ◽  
Vol 8 (09) ◽  
pp. 1081-1088 ◽  
Author(s):  
Elena De Carolis ◽  
Antonietta Vella ◽  
Luisa Vaccaro ◽  
Riccardo Torelli ◽  
Teresa Spanu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drug Susceptibility ◽  
Biochemical Tests ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Bacteria And Fungi ◽  
Diagnostic Microbiology ◽  
Tof Ms

Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently emerged as a powerful technique for identification of microorganisms, changing the workflow of well-established laboratories so that its impact on microbiological diagnostics has been unparalleled. In comparison with conventional identification methods that rely on biochemical tests and require long incubation procedures, MALDI-TOF MS has the advantage of identifying bacteria and fungi directly from colonies grown on culture plates in a few minutes and with simple procedures. Numerous studies on different systems available demonstrate the reliability and accuracy of the method, and new frontiers have been explored besides microbial species level identification, such as direct identification of pathogens from positive blood cultures, subtyping, and drug susceptibility detection.

scholarly journals Identification and dereplication of endophytic Colletotrichum strains by MALDI TOF mass spectrometry and molecular networking

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Morgane Barthélemy ◽  
Vincent Guérineau ◽  
Grégory Genta-Jouve ◽  
Mélanie Roy ◽  
Jérôme Chave ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Chemical Study ◽  
Chemical Diversity ◽  
Biologically Active ◽  
Ionization Time ◽  
Ion Clusters ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Palm Species ◽  
Ethyl Acetate Extracts

AbstractThe chemical diversity of biologically active fungal strains from 42 Colletotrichum, isolated from leaves of the tropical palm species Astrocaryum sciophilum collected in pristine forests of French Guiana, was investigated. The collection was first classified based on protein fingerprints acquired by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) correlated with cytotoxicity. Liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-HRMS/MS) data from ethyl acetate extracts were acquired and processed to generate a massive molecular network (MN) using the MetGem software. From five Colletotrichum strains producing cytotoxic specialized metabolites, we predicted the occurrence of peptide and cytochalasin analogues in four of them by MN, including a similar ion clusters in the MN algorithm provided by MetGem software. Chemoinformatics predictions were fully confirmed after isolation of three pentacyclopeptides (cyclo(Phe-Leu-Leu-Leu-Val), cyclo(Phe-Leu-Leu-Leu-Leu) and cyclo(Phe-Leu-Leu-Leu-Ile)) and two cytochalasins (cytochalasin C and cytochalasin D) exhibiting cytotoxicity at the micromolar concentration. Finally, the chemical study of the last active cytotoxic strain BSNB-0583 led to the isolation of four colletamides bearing an identical decadienamide chain.

scholarly journals First Isolation of Exiguobacterium aurantiacum in Serbia

2021 ◽  
Vol 70 (3) ◽  
pp. 405-407
Author(s):  
VERA P. GUSMAN ◽  
DEANA D. MEDIĆ ◽  
ANIKA DJ TRUDIĆ ◽  
PAVLE Z. BANOVIĆ ◽  
NATAŠA M. NIKOLIĆ
Keyword(s):  
Mass Spectrometry ◽  
Environmental Samples ◽  
Automated System ◽  
Distilled Water ◽  
Immunocompromised Patients ◽  
Bacterial Strains ◽  
Ionization Time ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Rare Bacteria

Exiguobacterium aurantiacum is isolated from a variety of environmental samples but rarely from patients. The aim of the study was to represent isolation of unusual bacterial strains that could cause infection in patients. Final identification was performed using matrix-assisted description/ionization time-of-flight mass spectrometry (MALDI-TOF). Two isolates strains of E. aurantiacum were isolated, one isolate from distilled water used during surgical treatment and the second one from a patient with bacteremia after radical prostatectomy, both sensitive to all tested antimicrobials. Environmental strains could cause infection, especially in immunocompromised patients; therefore, rare bacteria testing is required, in which identification special assistance is provided by an automated system MALDI-TOF.

scholarly journals Current Status of Matrix-Assisted Laser Desorption/Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in Clinical Diagnostic Microbiology

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4775
Author(s):  
Sachio Tsuchida ◽  
Hiroshi Umemura ◽  
Tomohiro Nakayama
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Clinical Applications ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms ◽  
Matrix Assisted Laser

Mass spectrometry (MS), a core technology for proteomics and metabolomics, is currently being developed for clinical applications. The identification of microorganisms in clinical samples using matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS) is a representative MS-based proteomics application that is relevant to daily clinical practice. This technology has the advantages of convenience, speed, and accuracy when compared with conventional biochemical methods. MALDI-TOF MS can shorten the time used for microbial identification by about 1 day in routine workflows. Sample preparation from microbial colonies has been improved, increasing the accuracy and speed of identification. MALDI-TOF MS is also used for testing blood, cerebrospinal fluid, and urine, because it can directly identify the microorganisms in these liquid samples without prior culture or subculture. Thus, MALDI-TOF MS has the potential to improve patient prognosis and decrease the length of hospitalization and is therefore currently considered an essential tool in clinical microbiology. Furthermore, MALDI-TOF MS is currently being combined with other technologies, such as flow cytometry, to expand the scope of clinical applications.

Can Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF) Enhance Antimicrobial Stewardship Efforts in the Acute Care Setting?

2013 ◽  
Vol 34 (9) ◽  
pp. 990-995 ◽  
Author(s):  
Pranita D. Tamma ◽  
Kennard Tan ◽  
Veronique R. Nussenblatt ◽  
Alison E. Turnbull ◽  
Karen C. Carroll ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

We evaluated 222 hospitalized patients whose clinical isolates were tested using standard methods and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF). MALDI-TOF could have reduced time to appropriate therapy for 28.8% and 44.6% patients based on the treating physician's choices and stewardship team recommendations, respectively. Clinicians should be aware of scenarios in which MALDI-TOF can optimize antibiotic therapy.

scholarly journals Iodine-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Sai Raghuveer Chava
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Desorption Ionization ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Adsorption and desorption of iodine-containing α-cyano-4-hydroxycinnamic acid (aCCa) matrix species were studied using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The MALDI-TOF MS method showed that ca. 0.8- 1.4 monolayer (~ 100 ppm) of iodine-containing species was adsorbed at the surface and assisted in desorption and ionization of a protein digest peptides or peptides varying in isoelectric potential. At low laser power, desorption of analytes in protonated and sodiated form was observed but not iodine cluster relative to aCCa without iodine, suggesting a two-electron reduction process to form the protonated pseudo molecular ion, although adsorption on the surface would lead to oxidation of iodide to iodine. The addition of iodine to matrix has been demonstrated to greatly facilitate the MALDI-TOF MS process and is a valuable tool when complex protein mixtures need to be analyzed.

scholarly journals A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification

2019 ◽  
Vol 5 (1) ◽  
pp. 4 ◽  
Author(s):  
Robin Patel
Keyword(s):  
Mass Spectrometry ◽  
Filamentous Fungi ◽  
Bacterial Identification ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Fungal Identification ◽  
Routine Identification ◽  
Tof Ms

As a result of its being inexpensive, easy to perform, fast and accurate, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) is quickly becoming the standard means of bacterial identification from cultures in clinical microbiology laboratories. Its adoption for routine identification of yeasts and even dimorphic and filamentous fungi in cultures, while slower, is now being realized, with many of the same benefits as have been recognized on the bacterial side. In this review, the use of MALDI-ToF MS for identification of yeasts, and dimorphic and filamentous fungi grown in culture will be reviewed, with strengths and limitations addressed.

scholarly journals Use of matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis of serum peptidome to classify and predict COVID-19 severity

2021 ◽  
Author(s):  
Rosa M Gomila ◽  
Gabriel Martorell ◽  
Pablo A Fraile-Ribot ◽  
Antonio Doménech-Sánchez ◽  
Miguel Albertí ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Critical Patients ◽  
Tof Ms ◽  
Matrix Assisted Laser

Abstract Background Classification and early detection of severe COVID-19 patients is required to establish an effective treatment. We tested the utility of matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to classify and predict the severity of COVID-19. Methods We used MALDI-TOF MS to analyse the serum peptidome from 72 COVID-19 patients (training cohort), clinically classified as mild (28), severe (23) and critical (21), and 20 healthy controls. The resulting matrix of peak intensities was used for Machine Learning (ML) approaches to classify and predict COVID-19 severity of 22 independent patients (validation cohort). Finally, we analysed all sera by liquid chromatography mass spectrometry (LC MS/MS) to identify the most relevant proteins associated to disease severity. Results We found a clear variability of the serum peptidome profile depending on COVID-19 severity. Forty-two peaks exhibited a log fold change ≥ 1 and 17 were significantly different and at least four-fold more intense in the set of critical patients than in the mild ones. ML approach classified clinical stable patients according to their severity with a 100% of accuracy and predicted correctly the evolution of the non-stable patients in all cases. LC MS/MS identified five proteins that were significantly upregulated in the critical patients. They included the serum amyloid protein A2, which probably yielded the most intense peak detected by MALDI-TOF MS. Conclusion We demonstrated the potential of the MALDI-TOF MS as a bench to bedside technology to aid clinicians in their decisions on COVID-19 patients.

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