scholarly journals Developing a Tooth in situ Organ Culture Model for Dental and Periodontal Regeneration Research

2021 ◽  
Vol 8 ◽  
Author(s):  
Reem El-Gendy ◽  
Sarah Junaid ◽  
Stephen K. L. Lam ◽  
Karen M. Elson ◽  
Joanne L. Tipper ◽  
...  
Keyword(s):  
Organ Culture ◽  
Culture System ◽  
Periodontal Regeneration ◽  
Glucose Consumption ◽  
Histomorphometric Analysis ◽  
Tissue Viability ◽  
Organ Culture System ◽  
Diffusion Characteristics ◽  
Culture Model

In this study we have realized the need for an organ culture tooth in situ model to simulate the tooth structure especially the tooth attachment apparatus. The importance of such a model is to open avenues for investigating regeneration of the complex tooth and tooth attachment tissues and to reduce the need for experimental animals in investigating dental materials and treatments in the future. The aim of this study was to develop a porcine tooth in situ organ culture model and a novel bioreactor suitable for future studies of periodontal regeneration, including application of appropriate physiological loading. The Objectives of this study was to establish tissue viability, maintenance of tissue structure, and model sterility after 1 and 4 days of culture. To model diffusion characteristics within the organ culture system and design and develop a bioreactor that allows tooth loading and simulation of the chewing cycle.Methods: Twenty-one porcine first molars were dissected aseptically in situ within their bony sockets. Twelve were used to optimize sterility and determine tissue viability. The remainder were used in a 4-day organ culture study in basal medium. Sterility was determined for medium samples and swabs taken from all tissue components, using standard aerobic and anaerobic microbiological cultures. Tissue viability was determined at days 1 and 4 using an XTT assay and Glucose consumption assays. Maintenance of structure was confirmed using histology and histomorphometric analysis. Diffusion characteristics were investigated using micro-CT combined with finite element modeling. A suitable bioreactor was designed to permit longer term culture with application of mechanical loading to the tooth in situ.Result: XTT and Glucose consumption assays confirmed viability throughout the culture period for all tissues investigated. Histological and histomorphometric analysis confirmed maintenance of tissue structure. Clear microbiological cultures indicated maintenance of sterility within the organ culture system. The novel bioreactor showed no evidence of medium contamination after 4 days of culture. Finite element modeling indicated nutrient availability to the periodontium.Conclusion: A whole tooth in situ organ culture system was successfully maintained over 4 days in vitro.

THE EFFECT OF OESTROGENS ON THE RESPONSE OF BONE TO PARATHYROID HORMONE IN VITRO

1972 ◽  
Vol 54 (1) ◽  
pp. 107-117 ◽  
Author(s):  
D. ATKINS ◽  
JOAN M. ZANELLI ◽  
M. PEACOCK ◽  
B. E. C. NORDIN
Keyword(s):  
Parathyroid Hormone ◽  
Citric Acid ◽  
Organ Culture ◽  
Culture System ◽  
Glucose Consumption ◽  
Organ Culture System ◽  
Mouse Calvaria ◽  
Ethinyl Oestradiol

SUMMARY An organ culture system was used to examine the effects of oestrogens on the response of 5-day-old mouse calvaria to parathyroid hormone (PTH). Parathyroid hormone released calcium and phosphate from the bone and this was associated with an increase in glucose consumption, an accumulation of citric acid and an inhibition of citrate oxidation. Oestradiol, oestriol, oestrone and ethinyl oestradiol all inhibited the PTH-induced release of calcium. The accumulation of citrate was prevented without the PTH-induced block on citrate oxidation being removed, and this was explained in terms of a reduction in glycolysis. Oestradiol, oestriol and oestrone appeared to be of equal potency. However, ethinyl oestradiol was active at much lower doses but appeared to be toxic at higher levels.

The isolation and in situ identification of MSCs residing in loose connective tissues using a niche-preserving organ culture system

Biomaterials ◽  
2012 ◽  
Vol 33 (18) ◽  
pp. 4469-4479 ◽  
Author(s):  
Min-Young Choi ◽  
Hyeong-In Kim ◽  
Young-Il Yang ◽  
Jong Tae Kim ◽  
Soo Hwa Jang ◽  
...  
Keyword(s):  
Organ Culture ◽  
Culture System ◽  
Connective Tissues ◽  
Organ Culture System

scholarly journals An organ culture system to model early degenerative changes of the intervertebral disc II: profiling global gene expression changes

2013 ◽  
Vol 15 (5) ◽  
pp. R121 ◽  
Author(s):  
Dessislava Z Markova ◽  
Christopher K Kepler ◽  
Sankar Addya ◽  
Hallie B Murray ◽  
Alexander R Vaccaro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture System ◽  
Global Gene Expression ◽  
Degenerative Changes ◽  
Organ Culture System

scholarly journals Development of an intact intervertebral disc organ culture system in which degeneration can be induced as a prelude to studying repair potential

2011 ◽  
Vol 20 (8) ◽  
pp. 1244-1254 ◽  
Author(s):  
Bernice Jim ◽  
Thomas Steffen ◽  
Janet Moir ◽  
Peter Roughley ◽  
Lisbet Haglund
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture System ◽  
Organ Culture System

scholarly journals In the absence of a basal lamina, ameloblasts absorb enamel in a serumless and chemically defined organ culture system

2021 ◽  
Author(s):  
Retsu Ohki ◽  
M. Matsuki-Fukushima ◽  
K. Fujikawa ◽  
Mitsuori Mayahara ◽  
Kayo Matsuyama ◽  
...  
Keyword(s):  
Organ Culture ◽  
Basal Lamina ◽  
Culture System ◽  
Organ Culture System

Bench-Top Validation Tests for Tissue-Engineered Arteries

2000 ◽  
Author(s):  
Shawn Chin Quee ◽  
Hai-Chao Han ◽  
David N. Ku
Keyword(s):  
Organ Culture ◽  
Culture System ◽  
Comprehensive Evaluation ◽  
Ex Vivo ◽  
Vascular Grafts ◽  
Living System ◽  
Organ Culture System ◽  
Flow Pressure ◽  
Validation Tests

Abstract Standard tests are needed for evaluating and comparing the mechanical and biological functions of tissue engineered arteries and other vascular grafts. We propose an ex vivo organ culture system as a living system for testing tissue-engineered vascular grafts. This bench-top organ culture system mimics the physiological environment of arteries including the flow, pressure, and the axial stretch. Arterial mechanical properties and physiologic functions including compliance, burst pressure, and contractile functions can be assessed before an expensive long-term animal test is initiated. Test results of natural arteries indicate that organ culture is a valid model for comprehensive evaluation of tissue-engineered vascular grafts.

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