scholarly journals Recent Trends in Biofabrication Technologies for Studying Skeletal Muscle Tissue-Related Diseases

2021 ◽  
Vol 9 ◽  
Author(s):  
Seungyeun Cho ◽  
Jinah Jang
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Motor Neurons ◽  
Close Contact ◽  
Human Muscle ◽  
Skeletal Muscle Tissue ◽  
Mechanism Study ◽  
Muscle Diseases ◽  
Muscle Models

In native skeletal muscle, densely packed myofibers exist in close contact with surrounding motor neurons and blood vessels, which are embedded in the fibrous connective tissue. In comparison to conventional two-dimensional (2D) cultures, the three-dimensional (3D) engineered skeletal muscle models allow structural and mechanical resemblance with native skeletal muscle tissue by providing geometric confinement and physiological matrix stiffness to the cells. In addition, various external stimuli applied to these models enhance muscle maturation along with cell–cell and cell–extracellular matrix interaction. Therefore, 3D in vitro muscle models can adequately recapitulate the pathophysiologic events occurring in tissue–tissue interfaces inside the native skeletal muscle such as neuromuscular junction. Moreover, 3D muscle models can induce pathological phenotype of human muscle dystrophies such as Duchenne muscular dystrophy by incorporating patient-derived induced pluripotent stem cells and human primary cells. In this review, we discuss the current biofabrication technologies for modeling various skeletal muscle tissue-related diseases (i.e., muscle diseases) including muscular dystrophies and inflammatory muscle diseases. In particular, these approaches would enable the discovery of novel phenotypic markers and the mechanism study of human muscle diseases with genetic mutations.

scholarly journals Recycled algae-based carbon materials as electroconductive 3D printed skeletal muscle tissue engineering scaffolds

2021 ◽  
Vol 32 (7) ◽  
Author(s):  
Selva Bilge ◽  
Emre Ergene ◽  
Ebru Talak ◽  
Seyda Gokyer ◽  
Yusuf Osman Donar ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Muscle Tissue ◽  
Carbonaceous Material ◽  
Hydrothermal Carbonization ◽  
Skeletal Muscle Tissue ◽  
Myotube Formation ◽  
3D Printed

AbstractSkeletal muscle is an electrically and mechanically active tissue that contains highly oriented, densely packed myofibrils. The tissue has self-regeneration capacity upon injury, which is limited in the cases of volumetric muscle loss. Several regenerative therapies have been developed in order to enhance this capacity, as well as to structurally and mechanically support the defect site during regeneration. Among them, biomimetic approaches that recapitulate the native microenvironment of the tissue in terms of parallel-aligned structure and biophysical signals were shown to be effective. In this study, we have developed 3D printed aligned and electrically active scaffolds in which the electrical conductivity was provided by carbonaceous material (CM) derived from algae-based biomass. The synthesis of this conductive and functional CM consisted of eco-friendly synthesis procedure such as pre-carbonization and multi-walled carbon nanotube (MWCNT) catalysis. CM obtained from biomass via hydrothermal carbonization (CM-03) and its ash form (CM-03K) were doped within poly(ɛ-caprolactone) (PCL) matrix and 3D printed to form scaffolds with aligned fibers for structural biomimicry. Scaffolds were seeded with C2C12 mouse myoblasts and subjected to electrical stimulation during the in vitro culture. Enhanced myotube formation was observed in electroactive groups compared to their non-conductive counterparts and it was observed that myotube formation and myotube maturity were significantly increased for CM-03 group after electrical stimulation. The results have therefore showed that the CM obtained from macroalgae biomass is a promising novel source for the production of the electrically conductive scaffolds for skeletal muscle tissue engineering.

scholarly journals Ether-Oxygen Containing Electrospun Microfibrous and Sub-Microfibrous Scaffolds Based on Poly(butylene 1,4-cyclohexanedicarboxylate) for Skeletal Muscle Tissue Engineering

2018 ◽  
Vol 19 (10) ◽  
pp. 3212 ◽  
Author(s):  
Nora Bloise ◽  
Emanuele Berardi ◽  
Chiara Gualandi ◽  
Elisa Zaghi ◽  
Matteo Gigli ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Muscle Tissue ◽  
Skeletal Muscle Tissue ◽  
Cell Alignment ◽  
Fibre Direction ◽  
Electrospun Scaffolds ◽  
Fibre Morphology

We report the study of novel biodegradable electrospun scaffolds from poly(butylene 1,4-cyclohexandicarboxylate-co-triethylene cyclohexanedicarboxylate) (P(BCE-co-TECE)) as support for in vitro and in vivo muscle tissue regeneration. We demonstrate that chemical composition, i.e., the amount of TECE co-units (constituted of polyethylene glycol-like moieties), and fibre morphology, i.e., aligned microfibrous or sub-microfibrous scaffolds, are crucial in determining the material biocompatibility. Indeed, the presence of ether linkages influences surface wettability, mechanical properties, hydrolytic degradation rate, and density of cell anchoring points of the studied materials. On the other hand, electrospun scaffolds improve cell adhesion, proliferation, and differentiation by favouring cell alignment along fibre direction (fibre morphology), also allowing for better cell infiltration and oxygen and nutrient diffusion (fibre size). Overall, C2C12 myogenic cells highly differentiated into mature myotubes when cultured on microfibres realised with the copolymer richest in TECE co-units (micro-P73 mat). Lastly, when transplanted in the tibialis anterior muscles of healthy, injured, or dystrophic mice, micro-P73 mat appeared highly vascularised, colonised by murine cells and perfectly integrated with host muscles, thus confirming the suitability of P(BCE-co-TECE) scaffolds as substrates for skeletal muscle tissue engineering.

Osteoprogenitor cells of mature human skeletal muscle tissue: an in vitro study

Bone ◽  
2001 ◽  
Vol 29 (4) ◽  
pp. 317-322 ◽  
Author(s):  
M.M Levy ◽  
C.J Joyner ◽  
A.S Virdi ◽  
A Reed ◽  
J.T Triffitt ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Human Skeletal Muscle ◽  
In Vitro Study ◽  
Skeletal Muscle Tissue ◽  
Vitro Study ◽  
Osteoprogenitor Cells

scholarly journals A Cylindrical Molding Method for the Biofabrication of Plane-Shaped Skeletal Muscle Tissue

Micromachines ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1411
Author(s):  
Minghao Nie ◽  
Ai Shima ◽  
Kenta Fukushima ◽  
Yuya Morimoto ◽  
Shoji Takeuchi
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Myogenic Differentiation ◽  
Skeletal Muscle Tissue ◽  
Differentiation Markers ◽  
Animal Products ◽  
Molding Method ◽  
Muscle Tissues ◽  
Central Pillar

Muscle tissues can be fabricated in vitro by culturing myoblast-populated hydrogels. To counter the shrinkage of the myoblast-populated hydrogels during culture, a pair of anchors are generally utilized to fix the two ends of the hydrogel. Here, we propose an alternative method to counter the shrinkage of the hydrogel and fabricate plane-shaped skeletal muscle tissues. The method forms myoblast-populated hydrogel in a cylindrical cavity with a central pillar, which can prevent tissue shrinkage along the circumferential direction. By eliminating the usages of the anchor pairs, our proposed method can produce plane-shaped skeletal muscle tissues with uniform width and thickness. In experiments, we demonstrate the fabrication of plane-shaped (length: ca. 10 mm, width: 5~15 mm) skeletal muscle tissue with submillimeter thickness. The tissues have uniform shapes and are populated with differentiated muscle cells stained positive for myogenic differentiation markers (i.e., myosin heavy chains). In addition, we show the assembly of subcentimeter-order tissue blocks by stacking the plane-shaped skeletal muscle tissues. The proposed method can be further optimized and scaled up to produce cultured animal products such as cultured meat.

Effect of flavonoids on protease activities in human skeletal muscle tissue in vitro

1999 ◽  
Vol 285 (1-2) ◽  
pp. 13-20 ◽  
Author(s):  
David Mantle ◽  
Gavin Falkous ◽  
Elaine K Perry
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Human Skeletal Muscle ◽  
Skeletal Muscle Tissue

scholarly journals Matrigel 3D bioprinting of contractile human skeletal muscle models recapitulating exercise and pharmacological responses

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Angela Alave Reyes-Furrer ◽  
Sonia De Andrade ◽  
Dominic Bachmann ◽  
Heidi Jeker ◽  
Martin Steinmann ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Muscle Wasting ◽  
Cooling System ◽  
Human Muscle ◽  
3D Bioprinting ◽  
Drop On Demand ◽  
Custom Made ◽  
Muscle Models

AbstractA key to enhance the low translatability of preclinical drug discovery are in vitro human three-dimensional (3D) microphysiological systems (MPS). Here, we show a new method for automated engineering of 3D human skeletal muscle models in microplates and functional compound screening to address the lack of muscle wasting disease medication. To this end, we adapted our recently described 24-well plate 3D bioprinting platform with a printhead cooling system to allow microvalve-based drop-on-demand printing of cell-laden Matrigel containing primary human muscle precursor cells. Mini skeletal muscle models develop within a week exhibiting contractile, striated myofibers aligned between two attachment posts. As an in vitro exercise model, repeated high impact stimulation of contractions for 3 h by a custom-made electrical pulse stimulation (EPS) system for 24-well plates induced interleukin-6 myokine expression and Akt hypertrophy pathway activation. Furthermore, the known muscle stimulators caffeine and Tirasemtiv acutely increase EPS-induced contractile force of the models. This validated new human muscle MPS will benefit development of drugs against muscle wasting diseases. Moreover, our Matrigel 3D bioprinting platform will allow engineering of non-self-organizing complex human 3D MPS.

scholarly journals Controllable assembly of skeletal muscle-like bundles through 3D bioprinting

2021 ◽  
Author(s):  
Tingting Fan ◽  
Shuo Wang ◽  
Zongmin Jiang ◽  
Shen Ji ◽  
Wenhua Cao ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
3D Printing ◽  
Muscle Tissue ◽  
Muscle Cells ◽  
Skeletal Muscle Tissue ◽  
Skeletal Muscle Cells ◽  
Muscle Fibres ◽  
Physical Factors

Abstract 3D printing is an effective technology for recreating skeletal muscle tissue in vitro. To achieve clinical skeletal muscle injury repair, relatively large volumes of highly aligned skeletal muscle cells are required; obtaining these is still a challenge. It is currently unclear how individual skeletal muscle cells and their neighbouring components co-ordinate to establish anisotropic architectures in highly homogeneous orientations. Here, we demonstrated a 3D printing strategy followed by sequential culture processes to engineer skeletal muscle tissue. The effects of confined printing on the skeletal muscle during maturation, which impacted the myotube alignment, myogenic gene expression, and mechanical forces, were observed. Our findings demonstrate the dynamic changes of skeletal muscle tissue during in vitro 3D construction and reveal the role of physical factors in the orientation and maturity of muscle fibres.

scholarly journals Human Muscle Precursor Cells Overexpressing PGC-1α Enhance Early Skeletal Muscle Tissue Formation

2017 ◽  
Vol 26 (6) ◽  
pp. 1103-1114 ◽  
Author(s):  
Deana Haralampieva ◽  
Souzan Salemi ◽  
Ivana Dinulovic ◽  
Tullio Sulser ◽  
Simon M. Ametamey ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Precursor Cells ◽  
Human Muscle ◽  
Skeletal Muscle Tissue ◽  
Tissue Formation ◽  
Muscle Precursor ◽  
Muscle Precursor Cells
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