scholarly journals Assessment of the Effects of Double-Stranded RNAs Corresponding to Multiple Vitellogenesis-Inhibiting Hormone Subtype I Peptides in Subadult Female Whiteleg Shrimp, Litopenaeus vannamei

2021 ◽  
Vol 12 ◽  
Author(s):  
Bong Jung Kang ◽  
Zakea Sultana ◽  
Marcy N. Wilder
Keyword(s):  
Gene Expression ◽  
Litopenaeus Vannamei ◽  
Transcriptional Repression ◽  
Transcriptional Silencing ◽  
Ovarian Maturation ◽  
Sinus Gland ◽  
Decapod Crustaceans ◽  
Homologous Genes ◽  
Whiteleg Shrimp ◽  
Subadult Female

Vitellogenesis-inhibiting hormone (VIH) negatively regulates reproduction in shrimp and other decapod crustaceans. In order to assess the effects of transcriptional silencing by multiple VIH subtype I sinus gland peptides (SGPs) on ovarian maturation in female whiteleg shrimp, Litopenaeus vannamei, we synthesized five dsRNAs targeting Liv-SGP-A, −B, −C, −F, and −G and injected them into subadults. The following treatments were employed: sgpG-dsRNA (targeting Liv-SGP-G), sgpC-dsRNA (targeting Liv-SGP-C), and mixed-dsRNA (targeting Liv-SGP-A, −B, and −F). The expression of Liv-SGP-G in eyestalks was significantly decreased at 10, 20, and 30 days after the injection of sgpG-dsRNA In addition, it was significantly decreased at 10 and 30 days after the injection of mixed-dsRNA. The expression of vitellogenin (Vg) gene expression in the ovaries, and concentrations of Vg protein in the hemolymph, were not changed by the administration of any dsRNA treatment (the ovaries remained immature in all treated individuals and contained mostly oogonia and previtellogenic oocytes). Although the administration of dsRNAs corresponding to multiple VIHs did not promote ovarian maturation, this is the first report of the co-transcriptional repression of Liv-SGP-G by the injection of dsRNA for homologous genes (Liv-SGP-A, −B, and −F). These results indicate that subadults can respond to the techniques of transcriptional silencing.

Purification of Sinus Gland Peptides Having Vitellogenesis-Inhibiting Activity from the Whiteleg Shrimp Litopenaeus Vannamei

2007 ◽  
Vol 9 (3) ◽  
pp. 360-369 ◽  
Author(s):  
Naoaki Tsutsui ◽  
Tsuyoshi Ohira ◽  
Ichiro Kawazoe ◽  
Akiyoshi Takahashi ◽  
Marcy N. Wilder
Keyword(s):  
Litopenaeus Vannamei ◽  
Inhibiting Activity ◽  
Sinus Gland ◽  
Whiteleg Shrimp

Ontogenesis of metabolic gene expression in whiteleg shrimp (Litopenaeus vannamei): New molecular tools for programming in the future

Aquaculture ◽  
2017 ◽  
Vol 479 ◽  
pp. 142-149 ◽  
Author(s):  
Luis Paulo Araujo Lage ◽  
Elisabeth Plagnes-Juan ◽  
Soraia Marques Putrino ◽  
Frederic Baron ◽  
Delphine Weissman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Litopenaeus Vannamei ◽  
Molecular Tools ◽  
Metabolic Gene ◽  
Metabolic Gene Expression ◽  
The Future ◽  
Whiteleg Shrimp

Gene expression and energetic metabolism changes in the whiteleg shrimp ( Litopenaeus vannamei ) in response to short‐term hypoxia

2019 ◽  
Author(s):  
Sergio Alan Ulaje ◽  
Liliana Rojo‐Arreola ◽  
Salvador E. Lluch‐Cota ◽  
Felipe Ascencio ◽  
Pedro Cruz‐Hernández ◽  
...  
Keyword(s):  
Gene Expression ◽  
Litopenaeus Vannamei ◽  
Energetic Metabolism ◽  
Short Term ◽  
Whiteleg Shrimp

Gene expression and protein levels of thioredoxin in the gills from the whiteleg shrimp (Litopenaeus vannamei) infected with two different viruses: The WSSV or IHHNV

2012 ◽  
Vol 32 (6) ◽  
pp. 1141-1147 ◽  
Author(s):  
Karina D. Garcia-Orozco ◽  
Arturo Sanchez-Paz ◽  
Emmanuel Aispuro-Hernandez ◽  
Silvia Gomez-Jimenez ◽  
Alonso Lopez-Zavala ◽  
...  
Keyword(s):  
Gene Expression ◽  
Litopenaeus Vannamei ◽  
Protein Levels ◽  
Whiteleg Shrimp

Transcriptional silencing of vitellogenesis-inhibiting hormone (VIH) subtype-I in the whiteleg shrimp, Litopenaeus vannamei

Aquaculture ◽  
2019 ◽  
Vol 506 ◽  
pp. 119-126 ◽  
Author(s):  
Bong Jung Kang ◽  
Sun-Hye Bae ◽  
Tomoya Suzuki ◽  
Shuhei Niitsu ◽  
Marcy N. Wilder
Keyword(s):  
Litopenaeus Vannamei ◽  
Transcriptional Silencing ◽  
Whiteleg Shrimp

scholarly journals Evaluation of Potential Probiotics Bacillus subtilis WB60, Pediococcus pentosaceus, and Lactococcus lactis on Growth Performance, Immune Response, Gut Histology and Immune-Related Genes in Whiteleg Shrimp, Litopenaeus vannamei

2020 ◽  
Vol 8 (2) ◽  
pp. 281 ◽  
Author(s):  
Seonghun Won ◽  
Ali Hamidoghli ◽  
Wonsuk Choi ◽  
Jinho Bae ◽  
Won Je Jang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Lactococcus Lactis ◽  
Litopenaeus Vannamei ◽  
Digestive Enzyme ◽  
Challenge Test ◽  
Digestive Enzyme Activity ◽  
Pediococcus Pentosaceus ◽  
Whiteleg Shrimp

An eight-week feeding trial was conducted to evaluate the effects of different dietary probiotic supplements in juvenile whiteleg shrimp, Litopenaeus vannamei. A basal control diet without probiotics (CON), and five other diets by supplementing Bacillus subtilis at 107 CFU/g diet (BS7), B. subtilis (BS8), Pediococcus pentosaceus (PP8), and Lactococcus lactis (LL8) at 108 CFU/g diet, and oxytetracycline (OTC) at 4 g/kg diet were used. Whiteleg shrimp with initial body weights of 1.41 ± 0.05 g (mean ± SD) were fed with these diets. Growth of shrimp fed BS8 and LL8 diets was significantly higher than those of shrimp fed the CON diet (p < 0.05). Superoxide dismutase activity in shrimp fed PP8 and LL8 diets was significantly higher than that of shrimp fed the CON diet (p < 0.05). Lysozyme activity of shrimp fed probiotics and OTC diets significantly improved compared to those on the CON diet (p < 0.05). The intestinal histology showed healthier guts for shrimp fed the probiotic diets (p < 0.05). Immune-related gene expression in shrimp fed BS8, PP8 and LL8 diets was recorded as significantly higher than that of shrimp fed CON and OTC diets (p < 0.05). Also, results of the challenge test for 7 days and the digestive enzyme activity of shrimp fed BS8, PP8, and LL8 were significantly improved compared to those on the CON diet (p < 0.05). Therefore, these results indicated that L. lactis at 108 CFU/g could be an ideal probiotic for whiteleg shrimp, and also B. subtilis WB60 and P. pentosaceus at 108 CFU/g could improve the growth, immunity, histology, gene expression, digestive enzyme activity, and disease resistance, while replacing antibiotics.

Label-free proteomic analysis revealed the mechanisms of protein oxidation induced by hydroxyl radicals in whiteleg shrimp (Litopenaeus vannamei) muscle

2021 ◽  
Author(s):  
Hui-min Lin ◽  
Xue-er Qi ◽  
Shan-shan Shui ◽  
Soottawat Benjakul ◽  
Santiago P. Aubourg ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Hydroxyl Radicals ◽  
Litopenaeus Vannamei ◽  
Protein Oxidation ◽  
Proteomic Analysis ◽  
Muscle Proteins ◽  
Label Free ◽  
Whiteleg Shrimp ◽  
The Stability ◽  
Oxidative Effects

The oxidative effects of hydroxyl radicals derived from a FeCl3/ascorbic acid/H2O2 system on the stability of muscle proteins in peeled shrimp (Litopenaeus vannamei) were investigated.

scholarly journals Repression of ESR1 through Actions of Estrogen Receptor Alpha and Sin3A at the Proximal Promoter

2009 ◽  
Vol 29 (18) ◽  
pp. 4949-4958 ◽  
Author(s):  
Stephanie J. Ellison-Zelski ◽  
Natalia M. Solodin ◽  
Elaine T. Alarid
Keyword(s):  
Gene Expression ◽  
Estrogen Receptor ◽  
Transcription Factor ◽  
Rna Polymerase Ii ◽  
Transcriptional Repression ◽  
Estrogen Receptor Alpha ◽  
Proximal Promoter ◽  
Receptor Alpha ◽  
Expression Of Genes ◽  
Activation Of Transcription

ABSTRACT Gene expression results from the coordinated actions of transcription factor proteins and coregulators. Estrogen receptor alpha (ERα) is a ligand-activated transcription factor that can both activate and repress the expression of genes. Activation of transcription by estrogen-bound ERα has been studied in detail, as has antagonist-induced repression, such as that which occurs by tamoxifen. How estrogen-bound ERα represses gene transcription remains unclear. In this report, we identify a new mechanism of estrogen-induced transcriptional repression by using the ERα gene, ESR1. Upon estrogen treatment, ERα is recruited to two sites on ESR1, one distal (ENH1) and the other at the proximal (A) promoter. Coactivator proteins, namely, p300 and AIB1, are found at both ERα-binding sites. However, recruitment of the Sin3A repressor, loss of RNA polymerase II, and changes in histone modifications occur only at the A promoter. Reduction of Sin3A expression by RNA interference specifically inhibits estrogen-induced repression of ESR1. Furthermore, an estrogen-responsive interaction between Sin3A and ERα is identified. These data support a model of repression wherein actions of ERα and Sin3A at the proximal promoter can overcome activating signals at distal or proximal sites and ultimately decrease gene expression.

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