scholarly journals Detection of Jaguar (Panthera onca) From Genetic Material in Drinking Water

2021 ◽  
Vol 9 ◽  
Author(s):  
Taylor M. Wilcox ◽  
Anthony Caragiulo ◽  
Joseph C. Dysthe ◽  
Thomas W. Franklin ◽  
Daniel H. Mason ◽  
...  
Keyword(s):  
Drinking Water ◽  
Genetic Material ◽  
Environmental Dna ◽  
Careful Consideration ◽  
Panthera Onca ◽  
Northern Mexico ◽  
Persistence Time ◽  
Dna Sampling ◽  
Northern Portion ◽  
Conservation Concern

Jaguar (Panthera onca) are of conservation concern and occur at very low densities in the northern portion of their range in northern Mexico and the southwestern United States. Environmental DNA sampling to detect genetic material from drinking water may be an effective approach for jaguar detection in these arid landscapes. Here we develop a qPCR assay for the detection of jaguar mitochondrial DNA, show that large quantities of DNA (mean 66,820 copies/L) can be found in the drinking water of captive animals, and observe detectable levels of DNA (80 copies/L) in a wild habitat with known jaguar populations. We suggest that environmental DNA sampling may represent a useful, complementary sampling tool for detection of rare jaguars, although effective application would require careful consideration of DNA persistence time in the environment.

Distribution and Prevalence ofMyxobolus cerebralisin Postfire Areas of Plumas National Forest: Utility of Environmental DNA Sampling

2018 ◽  
Vol 30 (2) ◽  
pp. 130-143 ◽  
Author(s):  
Christine A. Richey ◽  
Kirsten V. Kenelty ◽  
Kristina Van Stone Hopkins ◽  
Brittany N. Stevens ◽  
Beatriz Martínez-López ◽  
...  
Keyword(s):  
Environmental Dna ◽  
National Forest ◽  
Dna Sampling

scholarly journals Environmental DNA Sampling of Small‐Bodied Minnows: Performance Relative to Location, Species, and Traditional Sampling

2019 ◽  
Vol 39 (5) ◽  
pp. 1073-1085 ◽  
Author(s):  
Anthony T. Robinson ◽  
Yvette M. Paroz ◽  
Matthew J. Clement ◽  
Thomas W. Franklin ◽  
Joseph C. Dysthe ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Dna Sampling

scholarly journals Using Noninvasive Genetic Sampling to Survey Rare Butterfly Populations

Insects ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 311
Author(s):  
Caroline Storer ◽  
Jaret Daniels ◽  
Lei Xiao ◽  
Kristin Rossetti
Keyword(s):  
Captive Breeding ◽  
Low Cost ◽  
Genetic Material ◽  
Breeding Population ◽  
Sample Collection ◽  
Genetic Sampling ◽  
Miami Blue Butterfly ◽  
Analysis Laboratory ◽  
Conservation Concern ◽  
Future Evaluation

Advances in nondestructive genetic sampling techniques continue to offer new opportunities for studying organisms, particularly those of conservation concern where more traditional invasive sampling methods are often not available. As part of a proof-of-concept, we investigated the effectiveness of using the chorion from residual butterfly egg debris as a source of viable genetic material for analysis. Laboratory material from a captive breeding population of the federally endangered Miami blue butterfly (Cyclargus thomasi bethunebakeri) was used to test efficacy and refine the methodology. The resulting best practices were subsequently evaluated using field-collected material from extant north Florida populations of the at-risk frosted elfin butterfly (Callophyrs irus). Our results demonstrated that it is possible to extract DNA of sufficiently high quantity and quality for successful gene sequencing. We additionally describe a simple, low-cost, and reliable method of collecting and storing egg debris samples that can be consistently adopted for field or laboratory work as well as deployed with projects that have a larger geographic scope and/or involve citizen scientists. Potential limitations related to field sample collection are discussed as well as needs for future evaluation.

Comparing Efficiency of American Fisheries Society Standard Snorkeling Techniques to Environmental DNA Sampling Techniques

2017 ◽  
Vol 37 (3) ◽  
pp. 644-651 ◽  
Author(s):  
Roy M. Ulibarri ◽  
Scott A. Bonar ◽  
Chris Rees ◽  
Jon Amberg ◽  
Bridget Ladell ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Sampling Techniques ◽  
Dna Sampling

scholarly journals Optimal survey designs for environmental DNA sampling

2017 ◽  
Vol 9 (4) ◽  
pp. 1049-1059 ◽  
Author(s):  
William H. Lugg ◽  
Josh Griffiths ◽  
Anthony R. Rooyen ◽  
Andrew R. Weeks ◽  
Reid Tingley
Keyword(s):  
Environmental Dna ◽  
Dna Sampling ◽  
Survey Designs

scholarly journals Assessing the cost‐efficiency of environmental DNA sampling

2016 ◽  
Vol 7 (11) ◽  
pp. 1291-1298 ◽  
Author(s):  
Adam S. Smart ◽  
Andrew R. Weeks ◽  
Anthony R. Rooyen ◽  
Alana Moore ◽  
Michael A. McCarthy ◽  
...  
Keyword(s):  
Cost Efficiency ◽  
Environmental Dna ◽  
Dna Sampling ◽  
The Cost

scholarly journals Surveillance of human and swine adenovirus, human norovirus and swine circovirus in water samples in Santa Catarina, Brazil

2012 ◽  
Vol 10 (3) ◽  
pp. 445-452 ◽  
Author(s):  
L. A. T. Garcia ◽  
A. Viancelli ◽  
C. Rigotto ◽  
M. R. Pilotto ◽  
P. A. Esteves ◽  
...  
Keyword(s):  
Drinking Water ◽  
Quantitative Polymerase Chain Reaction ◽  
Plaque Assay ◽  
Genetic Material ◽  
Swine Manure ◽  
Human Adenovirus ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Human Norovirus ◽  
Impact Surface

Animal and human wastewater can potentially contaminate water sources and the treatment of drinking water may not effectively remove all contaminants, especially viruses. The purpose of the present study was to evaluate the viral contamination of water used for human and animal consumption in the city of Concórdia, located in southern Brazil. Porcine circovirus type 2 (PCV2), porcine adenovirus (PAdV), human adenovirus (HAdV) and human norovirus (NoV) were searched for using quantitative polymerase chain reaction (qPCR). HAdV-positive samples were tested for viral infectivity by plaque assay. The qPCR results showed that PAdV, PCV2 and HAdV genetic material were present in all sampling sites. NoV was absent in all samples. The presence of genetic material from PAdV and PCV2 was detected in 30% and 45% of the 36 analyzed samples, respectively, with an average of 102 gc mL–1 for PAdV and 104 gc mL–1 for PCV2. HAdV was present in 100% of the samples, with an average of 104 gc mL–1. However, in plaque assay, only 36% of the samples were positive. As viable particles of HAdV were found in drinking water, these results confirm that swine manure and human sewage impact surface water and groundwater, endangering water quality and indicating a potential risk to public health.

scholarly journals An environmental DNA sampling method for aye-ayes from their feeding traces

2018 ◽  
Vol 8 (18) ◽  
pp. 9229-9240 ◽  
Author(s):  
Megan L. Aylward ◽  
Alexis P. Sullivan ◽  
George H. Perry ◽  
Steig E. Johnson ◽  
Edward E. Louis
Keyword(s):  
Sampling Method ◽  
Environmental Dna ◽  
Dna Sampling

scholarly journals An evaluation of defluoridation technologies in the context of decision-making strategies

2021 ◽  
Author(s):  
Ann C Fantin
Keyword(s):  
Decision Making ◽  
Drinking Water ◽  
Careful Consideration ◽  
Government Officials ◽  
Local Conditions ◽  
Starting Point ◽  
Specific Community ◽  
The Government ◽  
Selection Of

Fluoride can be considered beneficial or harmful to human health dependant on the amount that is ingested. Many methods exist for the removal of excessive fluoride from drinking water; however, not all defluoridation methods are appropriate for the specific local conditions that may be present within a community. The objective of this research project is to evaluate various available defluoridation technologies against characteristics of a successful and sustainable technology, while considering decision-making strategies that may be employed by those selecting an appropriate defluoridation method to suit the characteristics of the specific community that is affected by fluoride-impacted drinking water. An evaluation of the selected defluoridation technologies supports the notion that there is not an all-encompassing defluoridation technology that would be applicable to all types of situations in which fluoride impacted groundwater is a concern. Water treatment methods for developing countries require careful consideration and selection of a sustainable solution so as to provide long-term benefits and applicability. Formal decision-making strategies would be useful tools at the government level to provide a starting point to determine which available defluoridation methods would be viable at the end user level. Researchers, government officials and, most importantly, local inhabitants of suffering areas must work together to achieve the common goal of clean and safe potable water.

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