Molecular Analysis of Disease-Responsive Genes Revealing the Resistance Potential Against Fusarium Wilt (Fusarium udum Butler) Dependent on Genotype Variability in the Leguminous Crop Pigeonpea

Background: The development of Fusarium wilt resistant varieties is a major challenge in pigeonpea breeding and need to be addressed on priority basis. In this study, efforts had been made to characterize the elite pigeonpea genotypes for Fusarium wilt resistance at morphological and molecular level. Methods: The present study was undertaken during kharif season of year 2018-19 at NEB, Crop Research Centre, G.B.P.U.A and T, Pantnagar. The experimental materials for present study consisted of 15 elite pigeonpea genotypes and three commercially grown popular varieties as checks. The molecular analysis was conducted during year 2019-20 and a susceptible variety BAHAR was used as check. The nine yield based indices were used for morphological analysis. The molecular analysis was carried out by using five already reported Fusarium wilt linked SSR markers. Result: The results revealed that the marker ASSR 363, ASSR 366, ASSR 1, ASSR 23 and ASSR 148 were highly effective in differentiating the resistant and susceptible genotypes of pigeonpea for wilt disease. On the basis of morphological and molecular studies, it was concluded that the genotype PA 626 was the most superior genotype as it not only yielded higher than all the three checks but also shown resistance against wilt at both phenotypic and genotypic level.

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Early Screening of Fusarium Wilt and Molecular Analysis of Banana Variants

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2018.703.271 ◽

2018 ◽

Vol 7 (03) ◽

pp. 2313-2321

Author(s):

H. Kishor ◽

G. Prabhuling ◽

Y.C. Abhijith ◽

N. Manjunatha ◽

Y.P. Prasad

Keyword(s):

Molecular Analysis ◽

Fusarium Wilt ◽

Early Screening

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Molecular insights on differential responses of antioxidant and pathogenesis-related genes in pigeonpea [Cajanus cajan (L.) Millsp.] to Fusarium wilt (Fusarium udum (L.)

10.21203/rs.2.16464/v1 ◽

2019 ◽

Cited By ~ 1

Author(s):

Koushik Biswas ◽

Roshan Kumar ◽

Avijit Tarafdar ◽

Vipin Gupta ◽

Nirjara Singhvi ◽

...

Keyword(s):

Fusarium Wilt ◽

In Silico ◽

Cajanus Cajan ◽

Molecular Level ◽

In Silico Analysis ◽

Improvement Program ◽

Fusarium Udum ◽

Innate Resistance ◽

Pathogenesis Related ◽

Silico Analysis

Abstract Background: Pigeonpea [Cajanus cajan (L.) Millsp.] is a major dietary protein source to a large vegetarian population in the world. However, Fusarium wilt (FW) caused by Fusarium udum Butler (FU) is one of the challenging factors to pigeonpea production. FW resistance in pigeonpea is restricted to specific agro-climatic zones. Therefore, exploring a superior pigeonpea genotype from landraces or local cultivars cultivated in various rural regions and validating its resistance response at biological and molecular level could be an alternative to crop improvement program. The present study was carried out to identify superior genotypes of pigeonpea through selection of innate resistance to FW using different biological and molecular approaches.Result: Five distinct genotypes of pigeonpea along with well-known one resistant (ICP2894) and susceptible (ICP2376) control were selected on the basis of percentage of FW disease incidence from three different states of India after an extensive survey work. Among them, Richa had shown very less FW incidence (10.0%) under genotype evaluation study and was further selected for experimental validation at molecular level for its innate resistance to FW. Molecular characterization and expression profiling of biotic stress responsive genes representing antioxidant enzymes (APX and SOD) and Pathogenesis related proteins (CHS and β-1,3-glucanase)families were carried out. Under FU challenged conditions in Richa, expression patterns of both AO genes found to be similar, while expression of both PR genes was gradually increased with inoculation time. Putative 3D protein structure of CHS and SOD were generated. Molecular docking with fungal effect or protein cellobio-hydrolase-c (CBH-c) also revealed that their role in blocking the hydrolytic activity of CBH-c during pathogen invasion. Conclusion: The obtained results of expression study and in-silico analysis showed that the higher level of induction of PR and AO genes and strong interaction of their putative proteins with fungal CBH-cprotein established their antifungal activity conferring early plant defense responses to restrict the pathogen growth in resistant genotype Richa. Our study displaying a strong and combinatorial approach involving biological assay, molecular experiment and in-silico analysis to identify a superior genotype of pigeonpea resistant to FW across a major biogeographic region.

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Fusarium udum. [Descriptions of Fungi and Bacteria].

IMI Descriptions of Fungi and Bacteria ◽

10.1079/dfb/20056400575 ◽

1978 ◽

Author(s):

C. Booth

Keyword(s):

Geographical Distribution ◽

Fusarium Wilt ◽

Cajanus Cajan ◽

Pigeon Pea ◽

Fusarium Udum ◽

Drying Up ◽

Disease Fusarium Wilt ◽

As If

Abstract A description is provided for Fusarium udum. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: Cajanus cajan. DISEASE: Fusarium wilt of pigeon pea, characterized by withering and drying up of green parts as if from drought. GEOGRAPHICAL DISTRIBUTION: India; the disease is soil-borne and restricted to the parts of India where the crop is grown. TRANSMISSION: A soil-borne disease but also transmitted on roots (Subramanian, 1971).

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