scholarly journals Structural Abnormalities of Spermatozoa in Triploid Gynogenetic Crucian Carp (Carassius auratus)

2021 ◽  
Vol 12 ◽  
Author(s):  
Wangchao He ◽  
Yu Sun ◽  
Jiaxu Qiang ◽  
Xinyue Luo ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Carassius Auratus ◽  
Crucian Carp ◽  
Quantitative Real Time Pcr ◽  
Artificial Breeding ◽  
Intrinsic Mechanism ◽  
Red Crucian Carp ◽  
Structural Abnormalities ◽  
Structural Differences ◽  
Abnormal Head ◽  
Molecular Expression

The spermatozoa of triploid gynogenetic crucian carp (Carassius auratus) (3nDTCC) possess a spermatogenesis process with a normal genetic background. However, the genetic materials of these spermatozoa do not completely inherit gynogenetic progeny in general. Understanding the intrinsic mechanism may be helpful for developing breeding strategies of gynogenetic fishes. In this study, the spermatozoa ultrastructure was systematically studied in diploid red crucian carp and 3nDTCC to demonstrate their cytological structural differences. In addition, the artificial breeding tests of 3nDTCC(♀) with different ploidy spermatozoa were performed to verify the contributions of genetic materials from 3nDTCC spermatozoa to the gynogenesis progeny. Furthermore, the mRNA expression of centriole-related genes (i.e., cep57, cetn1, rootletin, and nek2) involved in spermatozoa packaging was also determined by quantitative real-time PCR (qPCR) to illustrate the molecular expression characteristics of the spermatozoa packaging process in 3nDTCC. The results reveal the adaptive features of spermatozoa in 3nDTCC, including the loose midpiece structure, abnormal head structure, and abnormal expression of centriole-related genes, which may influence the motility of spermatozoa and make it not involved normally in the genetic composition of the gynogenesis offspring.

Heterogenetic tetraploids from a cross of Japanese phytophagous crucian carp females (Carassius auratus cuvieri) and red crucian carp males (Carassius auratus red var.)

Aquaculture ◽  
1993 ◽  
Vol 111 (1-4) ◽  
pp. 317-318
Author(s):  
Chen Minrong ◽  
Yang Xinqi ◽  
Yu Xiaomu ◽  
Liu Hanqin ◽  
Yi Yonglan ◽  
...  
Keyword(s):  
Carassius Auratus ◽  
Crucian Carp ◽  
Red Crucian Carp

scholarly journals Effects of two kinds of fishery drugs on the expressions of GAD and GABA-T mRNA in crucian carp (Carassius auratus gibelio)

2020 ◽  
Vol 46 (6) ◽  
pp. 1965-1973
Author(s):  
Fan Zhang ◽  
Kun Hu ◽  
Jianzhen Huang ◽  
Zhi Tan ◽  
Jiming Ruan
Keyword(s):  
Glutamate Decarboxylase ◽  
Carassius Auratus ◽  
Crucian Carp ◽  
Lethal Dose ◽  
Carassius Auratus Gibelio ◽  
Quantitative Real Time Pcr ◽  
Peripheral Tissues ◽  
Safe Dose ◽  
Safe Concentration ◽  
Different Tissues

Abstract The objective of this study was to investigate the effects of difloxacin (DIF) and avermectin (AVM) on glutamate decarboxylase (GAD) and GABA-transaminase (GABA-T) in different tissues of crucian carp (Carassius auratus gibelio). After the treatments of DIF and AVM, the mRNA expressions of GAD and GABA-T in different tissues were detected by quantitative real-time PCR (qPCR). The results showed that the mRNA expressions of GAD65, GAD67, and GABA-T in the telencephalon (Tel), mesencephalon (Mes), cerebella (Cer), and medulla oblongata (Med) were downregulated significantly with the safe dose (SD, 20 mg/kg) of DIF (P < 0.05 or P < 0.01). While the expressions of GAD65 and GAD67 in the kidney at 12 h had strikingly upregulated to 13.81 ± 1.06** and 150.67 ± 12.85** times. Treated with the lethal dose of 50% (LD50, 2840 mg/kg b. W.) of DIF, the mRNA expressions of GAD65, GAD67, and GABA-T in all tissues were increased significantly (P < 0.01). The results of AVM group showed that the mRNA expressions of GAD65, GAD67, and GABA-T both in the central and peripheral tissues were all remarkably downregulated at the safe concentration (SC, 0.0039 mg/L) and the lethal concentration of 50% (LC50, 0.039 mg/L), except for the mRNA inhibitions of GAD65, GAD67, and GABA-T in the muscle at 2 h which sharply downregulated to 0.20 ± 0.02ΔΔ × 10−2, 0.57 ± 0.06ΔΔ × 10−1 and 0.44 ± 0.02ΔΔ × 10−1, respectively (P < 0.01).

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