Porcine B Cell Subset Responses to Toll-like Receptor Ligands

Altered expression and function of the Toll-like receptor (TLR) homologue CD180 molecule in B cells have been associated with autoimmune disorders. In this study, we report decreased expression of CD180 at protein and mRNA levels in peripheral blood B cells of diffuse cutaneous systemic sclerosis (dcSSc) patients. To analyze the effect of CD180 stimulation, together with CpG (TLR9 ligand) treatment, on the phenotype defined by CD19/CD27/IgD/CD24/CD38 staining, and function (CD69 and CD180 expression, cytokine and antibody secretion) of B cell subpopulations, we used tonsillar B cells. After stimulation, we found reduced expression of CD180 protein and mRNA in total B cells, and CD180 protein in B cell subpopulations. The frequency of CD180+ cells was the highest in the CD19+CD27+IgD+ non-switched (NS) B cell subset, and they showed the strongest activation after anti-CD180 stimulation. Furthermore, B cell activation via CD180 induced IL-6 and natural autoantibody secretion. Treatment with the combination of anti-CD180 antibody and CpG resulted in increased IL-6 and IL-10 secretion and natural autoantibody production of B cells. Our results support the role of CD180 in the induction of natural autoantibody production, possibly by NS B cells, and suggest an imbalance between the pathologic and natural autoantibody production in SSc patients.

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TOLL-like receptor ligands stimulate aberrant class switch recombination in early B cell precursors

International Immunology ◽

10.1093/intimm/dxn117 ◽

2008 ◽

Vol 20 (12) ◽

pp. 1575-1585 ◽

Cited By ~ 9

Author(s):

E. Edry ◽

H. Azulay-Debby ◽

D. Melamed

Keyword(s):

B Cell ◽

Class Switch Recombination ◽

Toll Like Receptor ◽

Receptor Ligands ◽

Class Switch ◽

B Cell Precursors

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B cell activating factor is induced by toll-like receptor and NOD-like receptor-ligands and plays critical role in IgM synthesis in Labeo rohita

Molecular Immunology ◽

10.1016/j.molimm.2016.08.010 ◽

2016 ◽

Vol 78 ◽

pp. 9-26 ◽

Cited By ~ 11

Author(s):

Madhubanti Basu ◽

Saswati S Lenka ◽

Mahismita Paichha ◽

Bhakti Patel ◽

Rajanya Banerjee ◽

...

Keyword(s):

B Cell ◽

Labeo Rohita ◽

Critical Role ◽

Toll Like Receptor ◽

Receptor Ligands ◽

B Cell Activating Factor

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Toll-like receptor ligands sensitize B-cell receptor signalling by reducing actin-dependent spatial confinement of the receptor

Nature Communications ◽

10.1038/ncomms7168 ◽

2015 ◽

Vol 6 (1) ◽

Cited By ~ 54

Author(s):

Spencer A. Freeman ◽

Valentin Jaumouillé ◽

Kate Choi ◽

Brian E. Hsu ◽

Harikesh S. Wong ◽

...

Keyword(s):

B Cell ◽

Cell Receptor ◽

Single Particle Tracking ◽

Actin Dynamics ◽

Toll Like Receptor ◽

Microbial Infection ◽

Receptor Ligands ◽

Spatial Confinement ◽

Receptor Signalling ◽

Receptor Crosstalk

Abstract Integrating signals from multiple receptors allows cells to interpret the physiological context in which a signal is received. Here we describe a mechanism for receptor crosstalk in which receptor-induced increases in actin dynamics lower the threshold for signalling by another receptor. We show that the Toll-like receptor ligands lipopolysaccharide and CpG DNA, which are conserved microbial molecules, enhance signalling by the B-cell antigen receptor (BCR) by activating the actin-severing protein cofilin. Single-particle tracking reveals that increased severing of actin filaments reduces the spatial confinement of the BCR within the plasma membrane and increases BCR mobility. This allows more frequent collisions between BCRs and greater signalling in response to low densities of membrane-bound antigen. These findings implicate actin dynamics as a means of tuning receptor signalling and as a mechanism by which B cells distinguish inert antigens from those that are accompanied by indicators of microbial infection.

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Porphyromonas Gingivalis Lipopolysaccharide-induced B Cell Differentiation by Toll-like Receptors 2 and 4

Protein and Peptide Letters ◽

10.2174/0929866528666211118085828 ◽

2021 ◽

Vol 28 ◽

Author(s):

Sumei Liu ◽

Guojing Liu ◽

Qingxian Luan ◽

Yongping Ma ◽

Xiaoqian Yu

Keyword(s):

B Cells ◽

B Cell ◽

Cell Subset ◽

Mrna Levels ◽

B Cell Differentiation ◽

Toll Like Receptor ◽

Regulatory B Cells ◽

E Coli ◽

Porphyromonas Gingivalis Lipopolysaccharide

Background: : Porphyromonasgingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis. It induces periodontitis with bone loss as its main feature by changing the number and composition of symbiotic microorganisms, as well as inducing the natural immune response of the host. However, the mechanism of the latter remains unclear. Objective:: This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis. Method:: We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using flow cytometry. Through toll-like receptor (TLR) knockout mice, the role of TLR2 and TLR4 in this process were also evaluated. Results : Results showed that P. gingivalis stimulated B cells to produce IL-10 via TLR2/4. CD5+B1 subset is the main source of IL-10+Breg cell. Under P. gingivalis LPS stimulation, CD5+IgM+CD93-IL-10+B cell subset increased significantly which was regulated through TLR2/4. Conclusion: The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.

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