Postulating the Modality of Integrative Taxonomy in Describing the Cryptic Congener Pampus griseus (Cuvier) and Systematics of the Genus Pampus (Perciformes: Stromateidae)

Pomfrets (genus Pampus), a highly commercial fishery resource distributed in the Indo-Western Pacific that includes Lessepsian migrants, have witnessed a series of systematic reforms. In this study, based on comprehensive sampling spanning type localities and coevals in the Northern Indian Ocean, the cryptic and valid species Stromateus griseus is resurrected from the synonymy and re-described as Pampus griseus (New Combination) based on 35 specimens from the Bay of Bengal, corroborated by a molecular analysis, which indicated a confined distribution of the species. The Bayesian phylogeny of the genus was reconstructed, incorporating redressed barcodes (582 nucleotides) and concatenated mitochondrial gene sequence data (1,822 nucleotides) generated from the recorded species P. candidus, P. chinensis and the neophyte along with sequences from GenBank entrusting the latest literature. The phylograms differed in topology as for seven valid species, and the one predicated on the concatenated data erected a highly supported polytomous clade for the P. cinereus complex (P. griseus, P. cinereus, and P. candidus) which shares synapomorphies. Pampus argenteus and P. minor, together, formed a sister clade to the rest. Climate-driven vicariant events during glacial epochs and the Indo-Pacific Barrier effect can be the drivers behind the Indian and Pacific Ocean sister lineages in P. chinensis. A multivariate analysis isolated the cryptic species from its congeners. This article portrays the systematics revision of genus Pampus with an integrative taxonomic approach compiling distinctive molecular, morphological, and anatomical features, revised key for species identification, taxonomic archives of Indian stromateids, and winds up with specific remarks.

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A new genus, Perplexacara, and new generic placements of species of Australian marsh beetles (Coleoptera: Scirtidae) based on morphology and molecular genetic data

Zootaxa ◽

10.11646/zootaxa.4927.4.4 ◽

2021 ◽

Vol 4927 (4) ◽

pp. 539-548

Author(s):

C.H.S. WATTS ◽

T.M. BRADFORD ◽

S.J.B. COOPER

Keyword(s):

New Genus ◽

Sequence Data ◽

Mitochondrial Gene ◽

Molecular Genetic ◽

Elongation Factor ◽

New Combination ◽

Cytochrome Oxidase Subunit ◽

Molecular Genetic Data ◽

Elongation Factor 1 Alpha ◽

Elongation Factor 1

The Australian Scirtidae species previously identified as misplaced in the widespread genus Prionocyphon Redtenbacher are revisited as well as their possible relationship with the Australian genus Macrodascillus (Lea) using sequence data from the mitochondrial gene, cytochrome oxidase subunit 1 and two nuclear genes, elongation factor 1-alpha and Topoisomerase. The study confirmed the conclusion of Cooper et al. (2014) that the species did not belong in Prionocyphon. The study also included a species from each of three possibly related genera, Chameloscyphon Watts, Daploeuros Watts and Dasyscyphon Watts. Chameloscyphon huonensis Watts, Dasyscyphon victoriaensis Watts and Daploeuros lamingtonensis Watts were recovered as separate lineages with C. huonensis linking with Das. victoriaensis and Dap. lamingtonensis isolated. The species previously included in Prionocyphon were shown to belong in two genera, Macrodascillus and a new genus Perplexacara: Perplexacara caementum (Watts) new combination, P. latusmandibulara (Watts) new combination, P. macroflavida (Watts) new combination, Macrodascillus scalaris (Lea), M. insolitus (Watts) new combination and M. lamingtonensis (Watts) new combination.

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A new species of Tetragonopterus Cuvier, 1816 (Characiformes: Characidae: Tetragonopterinae) from the rio Jari, Amapá, northern Brazil

Neotropical Ichthyology ◽

10.1590/s1679-62252011000100002 ◽

2011 ◽

Vol 9 (1) ◽

pp. 49-56 ◽

Cited By ~ 38

Author(s):

Bruno F Melo ◽

Ricardo C Benine ◽

Tatiane C Mariguela ◽

Claudio Oliveira

Keyword(s):

New Species ◽

Mitochondrial Gene ◽

The Other ◽

New Combination ◽

Valid Species ◽

Caudal Peduncle ◽

C Subunit ◽

Northern Brazil ◽

Square Spot ◽

A New Species

A new species of Tetragonopterus is described from the rio Jari, a tributary to the left margin of rio Amazonas, at the border between Amapá and Pará States, northern Brazil. It is morphologically diagnosed from the other species of the genus (T. argenteus, T. chalceus, and T. rarus new combination) by the lozenge-shaped spot on the caudal peduncle vs. rounded to square spot on the other species. Partial sequences of the mitochondrial gene Cytochrome Oxidase C subunit I, from representatives of all valid species of Tetragonopterus, including this new species, were analyzed. The obtained results revealed a significant genetic distance between the new species and its congeners. A discussion on the new combination, Tetragonopterus rarus, is also provided.

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Integrative taxonomy reveals a cryptic species of the nudibranch genus Polycera (Polyceridae) in European waters

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315420000612 ◽

2020 ◽

Vol 100 (5) ◽

pp. 733-752

Author(s):

Cecilie Gotaas Sørensen ◽

Cessa Rauch ◽

Marta Pola ◽

Manuel António E. Malaquias

Keyword(s):

Species Delimitation ◽

Molecular Phylogenetics ◽

Mitochondrial Gene ◽

Haplotype Network ◽

Integrative Taxonomy ◽

Geographic Range ◽

Yellow Orange ◽

Anatomical Characters ◽

Taxonomic Approach ◽

Barcode Gap

AbstractThis work aimed to test whether the colour variability featured by the European nudibranch Polycera quadrilineata is consistent with the concept of a single polychromatic species or may hide multiple lineages. Samples from across the geographic range of P. quadrilineata together with representatives from worldwide species with a focus on Atlantic diversity, were gathered and studied using an integrative taxonomic approach. Morpho-anatomical characters were investigated by light and scanning electron microscopy. Bayesian molecular phylogenetics using MrBayes, the Automatic Barcode Gap Discovery species delimitation method, and haplotype network analysis using the PopArt software were employed to help delimit species using the mitochondrial gene cytochrome c oxidase subunit I (COI). The results supported the existence of a second species, here described and named Polycera norvegica sp. nov., only known from Norway where it is sympatric with P. quadrilineata. The COI uncorrected p-genetic distance between the two species was estimated at 9.6–12.4%. Polycera norvegica sp. nov. differs by exhibiting a black dotted or patchy dotted pattern occasionally with more or less defined orange/brown patches, but never black continuous or dashed stripes as in P. quadrilineata. The two species share a common colouration with a whitish base and yellow/orange tubercles. Anatomically, P. norvegica sp. nov. has a weaker labial cuticle, a smaller radula with fewer rows, and only four marginal teeth, a reproductive system with a single lobed bursa copulatrix, shorter reproductive ducts, and a penis armed with two kinds of spines: needle-like and hook-shaped penile spines.

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A new species of the genus Centruroides Marx (Scorpiones, Buthidae) from western Michoacán State, México using molecular and morphological evidence

ZooKeys ◽

10.3897/zookeys.859.33069 ◽

2019 ◽

Vol 859 ◽

pp. 31-48

Author(s):

Ana F. Quijano-Ravell ◽

Luis F. de Armas ◽

Oscar F. Francke ◽

Javier Ponce-Saavedra

Keyword(s):

New Species ◽

Mitochondrial Gene ◽

Morphological Characters ◽

Integrative Taxonomy ◽

Valid Species ◽

Morphological Evidence ◽

Molecular Evidence ◽

Mountain Range ◽

A New Species ◽

Gene Mrna

A new species of scorpion belonging to the genus Centruroides Marx, 1890 is described from the Coalcomán mountain range, western Michoacán State, Mexico. Its general aspect resembles Centruroidesruana Quijano-Ravell & Ponce-Saavedra, 2016, and C.infamatus (C. L. Koch, 1844), but it is a smaller species having lower pectinal tooth counts; also, males of C.ruana have the pedipalp chelae slightly thicker, whereas C.infamatus has a subaculear tubercle nearer to the base of the aculeus. Another species with similar aspect is Centruroidesornatus Pocock, 1902; however, a preliminary molecular analysis of the mitochondrial gene mRNA 16S showed genetic divergence (measured as p-distance) near to 10% between these species, and lower differences between the new species with respect to C.infamatus (4.63%) and C.ruana (5.07%). The molecular evidence together with the morphological characters (integrative taxonomy) are sufficient for recognizing the Coalcomán population as a separate and valid species.

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Apertochrysa (Neuroptera: Chrysopidae): A heterogeneric phantom?

Zootaxa ◽

10.11646/zootaxa.4238.1.4 ◽

2017 ◽

Vol 4238 (1) ◽

pp. 58 ◽

Cited By ~ 5

Author(s):

ATSUSHI MOCHIZUKI ◽

CHARLES S. HENRY ◽

PETER DUELLI

Keyword(s):

Type Species ◽

Morphological Traits ◽

New Genus ◽

Sequence Data ◽

New Combination ◽

Wing Venation ◽

Monophyletic Clade ◽

Molecular Sequence Data ◽

Molecular Sequence ◽

Group Relationships

The small lacewing genus Apertochrysa comprises species from Africa, Asia and Australia. All lack a tignum, but otherwise resemble distantly related genera. We show that Apertochrysa does not form a monophyletic clade, based on analyses of molecular sequence data and morphological traits such as the presence and shape of the male gonapsis, wing venation, and larval setae. Apertochrysa kichijoi forms a clade with Eremochrysa, Suarius and Chrysemosa, whereas A. albolineatoides belongs to a clade that includes Cunctochrysa. Apertochrysa albolineatoides should become a new combination as Cunctochrysa albolineatoides, while A. kichijoi will have to be transferred to a new genus. The Australian A. edwardsi, the African A. eurydera and the type species of the genus Apertochrysa, A. umbrosa, join the large Pseudomallada group. Relationships of A. umbrosa are less certain, because for it we could amplify only one of the three nuclear genes used in the overall analysis. However, in all morphological traits tested, that species strongly resembles A. edwardsi and A. eurydera and thus is very likely just another exceptional Pseudomallada lacking a tignum. The fate of the genus name Apertochrysa depends on additional molecular and morphological analyses of A. umbrosa.

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Colouration, chaetotaxy and molecular data provide species-level resolution in a species complex of Dicranocentrus (Collembola : Entomobryidae)

Invertebrate Systematics ◽

10.1071/is18019 ◽

2018 ◽

Vol 32 (6) ◽

pp. 1298 ◽

Cited By ~ 4

Author(s):

Feng Zhang ◽

Daoyuan Yu ◽

Mark I. Stevens ◽

Yinhuan Ding

Keyword(s):

Species Complex ◽

Instar Larva ◽

Molecular Data ◽

Integrative Taxonomy ◽

Species Level ◽

Integrative Approach ◽

Gene Markers ◽

Late Neogene ◽

Taxonomic Approach ◽

Colour Patterns

Integrative taxonomic approaches are increasingly providing species-level resolution to ‘cryptic’ diversity. In the absence of an integrative taxonomic approach, formal species validation is often lacking because of inadequate morphological diagnoses. Colouration and chaetotaxy are the most commonly used characters in collembolan taxonomy but can cause confusion in species diagnoses because these characters often have large intraspecific variation. Here, we take an integrative approach to the genus Dicranocentrus in China where four species have been previously recognised, but several members of the genus have been morphologically grouped as a species complex based on having paired outer teeth on unguis and seven colour patterns. Molecular delimitations based on distance- and evolutionary models recovered four candidate lineages from three gene markers and revealed that speciation events likely occurred during the late Neogene (4–13million years ago). Comparison of intact dorsal chaetotaxy, whose homologies were erected on the basis of first instar larva, further validated these candidates as formal species: D. gaoligongensis, sp. nov., D. similis, sp. nov., D. pallidus, sp. nov. and D. varicolor, sp. nov., and increase the number of Dicranocentrus species from China to eight. Our study further highlights the importance of adequate taxonomy in linking morphological and molecular characters within integrative taxonomy.

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Elusive Relationships Within Order Fabales: Phylogenetic Analyses Using matK and rbcL Sequence Data

Systematic Botany ◽

10.1600/036364409787602348 ◽

2009 ◽

Vol 34 (1) ◽

pp. 102-114 ◽

Cited By ~ 36

Author(s):

M. A. Bello ◽

A. Bruneau ◽

F. Forest ◽

J. A. Hawkins

Keyword(s):

Sequence Data ◽

Phylogenetic Analyses ◽

Morphological Evidence ◽

Taxon Sampling ◽

Rapid Radiation ◽

Bayesian Approaches ◽

Molecular Studies ◽

Alternative Hypotheses ◽

The One ◽

Good Support

The order Fabales, including Leguminosae, Polygalaceae, Quillajaceae and Surianaceae, represents a novel hypothesis emerging from angiosperm molecular phylogenies. Despite good support for the order, molecular studies to date have suggested contradictory, poorly supported interfamilial relationships. Our reappraisal of relationships within Fabales addresses past taxon sampling deficiencies, and employs parsimony and Bayesian approaches using sequences from the plastid regions rbcL (166 spp.) and matK (78 spp.). Five alternative hypotheses for interfamilial relationships within Fabales were recovered. The Shimodaira-Hasegawa test found the likelihood of a resolved topology significantly higher than the one calculated for a polytomy, but did not favour any of the alternative hypotheses of relationship within Fabales. In the light of the morphological evidence available and the comparative behavior of rbcL and matK, the topology recovering Polygalaceae as sister to the rest of the order Fabales with Leguminosae more closely related to Quillajaceae + Surianaceae, is considered the most likely hypothesis of interfamilial relationships of the order. Dating of selected crown clades in the Fabales phylogeny using penalized likelihood suggests rapid radiation of the Leguminosae, Polygalaceae, and (Quillajaceae + Surianaceae) crown clades.

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Relationships and revised status of Nagathrips (Thysanoptera: Phlaeothripinae)

Zootaxa ◽

10.11646/zootaxa.4231.4.8 ◽

2017 ◽

Vol 4231 (4) ◽

pp. 577

Author(s):

R. VARATHARAJAN ◽

TH. JOHNSON ◽

SINGH TARUNKUMAR

Keyword(s):

Sequence Data ◽

Mitochondrial Gene ◽

Antennal Segment ◽

Single Species ◽

Original Description ◽

Partial Sequence

The generic name Nagathrips was erected as a sub-genus of Mesothrips for the single species, crenulatus Varatharajan and Singh (2000). Subsequent studies revealed character states that were not included in the original description. These are: prosternal basantra absent; antennal segment III with one slender sense cone, segment IV with three similar sense cones; fore wings parallel-sided with no median constriction. These character states are not found in any species of Mesothrips, a genus of the Tribe Haplothripini (Mound & Minaei 2007), but are typical of members of the Liothrips-lineage (Dang et al. 2014). To confirm such a relationship partial sequence data of the mitochondrial gene (CO1) was obtained and compared with that of Gynaikothrips species.

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A revision of the genus Psammogorgia Verrill, 1868 (Cnidaria, Anthozoa, Octocorallia) in the tropical eastern Pacific Ocean

ZooKeys ◽

10.3897/zookeys.961.54846 ◽

2020 ◽

Vol 961 ◽

pp. 1-30

Author(s):

Odalisca Breedy ◽

Hector M. Guzman

Keyword(s):

Taxonomic Status ◽

Taxonomic Revision ◽

Eastern Pacific ◽

New Combination ◽

Valid Species ◽

Shallow Waters ◽

Eastern Pacific Ocean ◽

Tropical Eastern Pacific ◽

Character Tables ◽

Two New Species

The species of the genus Psammogorgia Verrill, 1868 from the shallow waters of the tropical eastern Pacific were mainly described from 1846 to 1870. Very few contributions were published subsequently. Recently, the genus was revisited with the addition of two new species. However, a comprehensive generic study is still missing for the eastern Pacific. Psammogorgia is characterised by having axes cores without mineralisation, mainly coarse irregular spindles and thorny, leafy or tuberculate clubs coenenchymal sclerites and the anthocodial armature with distinct collaret and points arrangements. Herein a taxonomic revision of the genus is presented based on type material which was morphologically analysed and illustrated using optical and scanning electron microscopy. Comparative character tables are provided for comparison among species in the genus, along with a taxonomic key. Moreover, the taxonomic status of each species was analysed. The genus Psammogorgia comprises six valid species and two varieties, and three lectotypes and a new combination are proposed to establish the taxonomic status of these species.

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Integrative taxonomy reveals a rare and new cusk-eel species of Luciobrotula (Teleostei, Ophidiidae) from the Solomon Sea, West Pacific

European Journal of Taxonomy ◽

10.5852/ejt.2021.750.1361 ◽

2021 ◽

Vol 750 ◽

pp. 52-69

Author(s):

Man-Kwan Wong ◽

Mao-Ying Lee ◽

Wei-Jen Chen

Keyword(s):

Lateral Line ◽

Gene Tree ◽

Distinct Species ◽

Integrative Taxonomy ◽

Coi Gene ◽

Valid Species ◽

The West ◽

West Pacific ◽

Fin Ray ◽

The Family

With six valid species, Luciobrotula is a small genus of the family Ophidiidae, commonly known as cusk-eels. They are benthopelagic fishes occurring at depths ranging from 115–2300 m in the Atlantic, Indian, and Pacific Oceans. Among them, Luciobrotula bartschi is the only known species in the West Pacific. Three specimens of Luciobrotula were collected from the Philippine Sea, Bismarck Sea, and Solomon Sea in the West Pacific during the AURORA, PAPUA NIUGINI, and MADEEP expeditions under the Tropical Deep-Sea Benthos program, and all of them were initially identified as L. bartschi. Subsequent examination with integrative taxonomy indicates that they belong to two distinct species, with the specimen collected from the Solomon Sea representing a new species, which is described here. In terms of morphology, Luciobrotula polylepis sp. nov. differs from its congeners by having a relatively longer lateral line (end of the lateral line below the 33rd dorsal-fin ray) and fewer vertebrae (abdominal vertebrae 13, total vertebrae 50). In the inferred COI gene tree, the two western Pacific species of Luciobrotula do not form a monophyletic group. The genetic K2P distance between the two species is 13.8% on average at the COI locus.

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