Culture System for a Closer Biological Contact Between Macrophages and Microparticles

To investigate the effects of microparticles on the immune system, a device was designed for an optimal culture environment. Macrophages phagocytose microparticles and produce inflammatory cytokines. In a culture environment in which macrophages phagocytose microparticles mixed in a culture medium, it remains unclear whether the macrophages can physically access all the microparticles present in the culture medium. In the culturing of fine particles, such as microparticles, it is necessary to devise methods that can realize a close biological contact between the macrophages and the microparticles. To enable macrophages to appropriately phagocytose microparticles, a microchamber with a glenoid hole for cell culturing was designed and manufactured. To clarify the effects of the size, administration amount, and administration time of the microparticles on the production of inflammatory cytokines, a system that can continuously deliver and collect the culture medium was introduced. The results obtained using these systems helped clarify the aforementioned effects. Our study confirms the possibility of employing a system that can optimally adjust the biological contact between macrophages and microparticles in a culture environment.

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Pro-inflammatory Cytokines, Biomarkers, Genetics and the Immune System: A Mechanistic Approach of Depression and Psoriasis

Revista Colombiana de Psiquiatría ◽

10.1016/j.rcp.2017.03.002 ◽

2018 ◽

Vol 47 (3) ◽

pp. 177-186 ◽

Cited By ~ 6

Author(s):

Daniyal Aleem ◽

Hassaan Tohid

Keyword(s):

Immune System ◽

Inflammatory Cytokines ◽

System A ◽

Mechanistic Approach ◽

Pro Inflammatory Cytokines

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Pro-inflammatory Cytokines, Biomarkers, Genetics and the Immune System: A Mechanistic Approach of Depression and Psoriasis

Revista Colombiana de Psiquiatría (English ed ) ◽

10.1016/j.rcpeng.2018.05.002 ◽

2018 ◽

Vol 47 (3) ◽

pp. 177-186

Author(s):

Daniyal Aleem ◽

Hassaan Tohid

Keyword(s):

Immune System ◽

Inflammatory Cytokines ◽

System A ◽

Mechanistic Approach ◽

Pro Inflammatory Cytokines

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239 CANINE OOCYTES CAN BE SPONTANEOUSLY PARTHENOGENERATED AND DEVELOPED TO 2-CELL TO 8-CELL STAGE PARTHENOGENERATED EMBRYOS IN COCULTURE SYSTEM WHICH THEY WERE COCULTURED WITH OVIDUCT CELLS OR OVIDUCT EXTRACTS

Reproduction Fertility and Development ◽

10.1071/rdv21n1ab239 ◽

2009 ◽

Vol 21 (1) ◽

pp. 217

Author(s):

L. X. Wang ◽

S. Wang ◽

J. Hou ◽

Y. F. Chen ◽

R. L. Hu ◽

...

Keyword(s):

In Vitro Culture ◽

Cell Volume ◽

Granulosa Cells ◽

Culture System ◽

Culture Medium ◽

Cell Stage ◽

Culture Systems ◽

System A ◽

Coculture System

The objective of this study was to coculture canine oocytes with oviduct cells or oviduct extracts in modified M199 culture medium to improve the MII rate. The canine oocytes were collected from the local slaughterhouse and were cultured in three culture systems while in the same culture medium. In culture system A, canine oocytes were cocultured with ‘small-round’ oviduct cells and ‘big-round’ oviductal cells which based on cell volume and morphology for 96 h. In culture system B, canine oocytes were cultured with in the modified M199 medium with 10% canine follicle fluid (in the CEF group) or without canine follicle fluid (in the compared group) for the first 6 h; following this, they were cocultured with ‘small-round’ oviductal cells for another 90 h, which were preblanced at least 2 h. In the culture system C, the canine oocytes were cocultured with canine estrous oviduct extracts for 96 h (in the compared group) or were cocultured with granulosa cells together with canine estrous oviduct extracts (in the G + CEOE group) for 96 h. These results demonstrated that, in culture system A, one 2-cell and one 8-cell stage parthenogenerated embryo in the small-round group and one 2-cell stage parthenogenerated embryo in the compared group were achieved after in vitro culture for 96 h. Meanwhile, the MII rates were greater in small-round group (47.1%) than that in big-round group (36.1%) and the compared group (32.4%). In the culture system B, one 3-cell stage parthenogenerated embryo was detected in CEF group. The MII rates were almost equal in CEF group (28.0%) and in the compared group (26.1%). In culture system C, one 2-cell stage parthenogenerated embryo was found in the compared group. The MII rate were almost equal in CEOE group (25.8%) and in the G + CEOE group (23.5%). It is concluded that some unidentified factors secreted by the oviductal cells or oviduct extracts may promote the development of canine oocytes in vitro and parthenogenerate the canine oocytes beyond 2-cell stage parthenogenerated embryos. The culture system in which the canine oocytes were cocultured with oviducts or oviductal cells has been improved to date.

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#23 Pro-inflammatory cytokines in the hippocampal neuronal cell layer of adult and aged mice after activation of the peripheral innate immune system: A laser capture microdissection study

Brain Behavior and Immunity ◽

10.1016/j.bbi.2005.10.029 ◽

2005 ◽

Vol 19 (4) ◽

pp. e12

Author(s):

J. Chen ◽

J.P. Godbout ◽

J. Abraham ◽

J.F. Zachary ◽

R.W. Johnson

Keyword(s):

Immune System ◽

Inflammatory Cytokines ◽

Laser Capture Microdissection ◽

Innate Immune System ◽

Cell Layer ◽

Neuronal Cell ◽

Innate Immune ◽

System A ◽

Laser Capture ◽

Pro Inflammatory Cytokines

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Twin boundary structure of a Y-Ba-Cu-O superconductor

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100152811 ◽

1989 ◽

Vol 47 ◽

pp. 168-169

Author(s):

Yimei Zhu ◽

Masaki Suenaga ◽

R. L. Sabatini ◽

Youwen Xu

Keyword(s):

Twin Boundary ◽

Fine Particles ◽

Imaging Techniques ◽

Fine Powder ◽

Orthorhombic Phase ◽

Boundary Structure ◽

Crystallographic Axis ◽

System A ◽

High Resolution Structure ◽

Electron Microscopes

The (110) twin structure of YBa2Cu3O7 superconductor oxide, which is formed to reduce the strain energy of the tetragonal to orthorhombic phase transformation by alternating the a-b crystallographic axis across the boundary, was extensively investigated. Up to now the structure of the twin boundary still remained unclear. In order to gain insight into the nature of the twin boundary in Y-Ba-Cu-O system, a study using electron diffraction techniques including optical and computed diffractograms, as well as high resolution structure imaging techniques with corresponding computer simulation and processing was initiated.Bulk samples of Y-Ba-Cu-O oxide were prepared as described elsewhere. TEM specimens were produced by crushing bulk samples into a fine powder, dispersing the powder in acetone, and suspending the fine particles on a holey carbon grid. The electron microscopy during this study was performed on both a JEOL 2000EX and 2000FX electron microscopes operated at 200 kV.

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