scholarly journals Concise Review: Considering Optimal Temperature for Short-Term Storage of Epithelial Cells

2021 ◽  
Vol 8 ◽  
Author(s):  
Ayyad Zartasht Khan ◽  
Tor Paaske Utheim ◽  
Catherine Joan Jackson ◽  
Kim Alexander Tønseth ◽  
Jon Roger Eidet
Keyword(s):  
Epithelial Cells ◽  
Storage Temperature ◽  
Freezing Point ◽  
Retinal Pigment ◽  
Cell Types ◽  
Retinal Pigment Epithelial Cells ◽  
Storage Medium ◽  
Short Term ◽  
Short Term Storage ◽  
Term Storage

Transplantation of novel tissue-engineered products using cultured epithelial cells is gaining significant interest. While such treatments can readily be provided at centralized medical centers, delivery to patients at geographically remote locations requires the establishment of suitable storage protocols. One important aspect of storage technology is temperature. This paper reviews storage temperature for above-freezing point storage of human epithelial cells for regenerative medicine purposes. The literature search uncovered publications on epidermal cells, retinal pigment epithelial cells, conjunctival epithelial cells, corneal/limbal epithelial cells, oral keratinocytes, and seminiferous epithelial cells. The following general patterns were noted: (1) Several studies across different cell types inclined toward 4 and 16°C being suitable short-term storage temperatures. Correspondingly, almost all studies investigating 37°C concluded that this storage temperature was suboptimal. (2) Cell death typically escalates rapidly following 7–10 days of storage. (3) The importance of the type of storage medium and its composition was highlighted by some of the studies; however, the relative importance of storage medium vs. storage temperature has not been investigated systematically. Although a direct comparison between the included investigations is not reasonable due to differences in cell types, storage media, and storage duration, this review provides an overview, summarizing the work carried out on each cell type during the past two decades.

Stress-induced ATP release from and growth modulation of human lens and retinal pigment epithelial cells

2003 ◽  
Vol 31 (6) ◽  
pp. 1213-1215 ◽  
Author(s):  
J.A. Eldred ◽  
J. Sanderson ◽  
M. Wormstone ◽  
J.R. Reddan ◽  
G. Duncan
Keyword(s):  
Epithelial Cells ◽  
Retinal Pigment ◽  
Atp Release ◽  
Cell Types ◽  
Cellular Systems ◽  
Retinal Pigment Epithelial Cells ◽  
Gene Profiling ◽  
Human Lens ◽  
Growth Modulation ◽  
Pigment Epithelial Cells

ATP release has been shown to occur following stimulation in several cellular systems. This study was undertaken to determine if lens and retinal epithelial cells release ATP in response to physiological stresses and to elucidate a possible role for ATP. Analysis of human aqueous humour samples showed a mean ATP level of 37.8±7.7 nM. Hyper-osmotic stress induced a dose- and time-dependent release of ATP. Both cell types were found to proliferate in serum-free medium, and the addition of ATP and adenosine at concentrations as low as 0.1 nM inhibited growth. Gene profiling also demonstrated the presence of the ectonucleotidases CD39 and CD73 and the A1 adenosine receptor on both cell types.

scholarly journals Changes in selected wine physical properties during the short-time storage 

2016 ◽  
Vol 62 (No. 3) ◽  
pp. 147-153 ◽  
Author(s):  
P. Hlaváč ◽  
M. Božiková ◽  
Z. Hlaváčová ◽  
K. Kardjilova
Keyword(s):  
Physical Properties ◽  
Storage Temperature ◽  
Thermal Parameters ◽  
Effect Of Temperature ◽  
Short Term ◽  
Electric Parameter ◽  
Short Term Storage ◽  
Temperature Manipulation ◽  
Dynamic And Kinematic Viscosity ◽  
Term Storage

This article is focused on the effect of temperature and short-term storage on the physical properties of wine made in Slovakia. All measurements were performed during temperature manipulation in the temperature interval approximately from 0°C to 30°C. Two series of rheologic and thermal parameters measurements and one of electric parameter were done. First measurement was done at the beginning of storage and then the same sample was measured after a short storage. Temperature relations of rheologic parameters and electric conductivity were characterized by exponential functions, which is in good agreement with the Arrhenius equation. In case of thermal parameters linear relations were obtained. The graphical dependency of wine density on temperature was described by decreasing polynomial function. The temperature dependencies of dynamic and kinematic viscosity have a decreasing character. The fluidity, thermal conductivity, thermal diffusivity, and electrical conductivity increased with the temperature. It was found out that short-term storage had a small effect on measured properties but longer storage could have a more significant influence on selected properties.  

scholarly journals Corrigendum: Concise Review: Considering Optimal Temperature for Short-Term Storage of Epithelial Cells

2021 ◽  
Vol 8 ◽  
Author(s):  
Ayyad Zartasht Khan ◽  
Tor Paaske Utheim ◽  
Catherine Joan Jackson ◽  
Kim Alexander Tønseth ◽  
Jon Roger Eidet
Keyword(s):  
Epithelial Cells ◽  
Optimal Temperature ◽  
Short Term ◽  
Concise Review ◽  
Short Term Storage ◽  
Term Storage

Short-term storage of cane toad (Bufo marinus) gametes

Reproduction ◽  
2001 ◽  
pp. 167-173 ◽  
Author(s):  
RK Browne ◽  
J Clulow ◽  
M Mahony
Keyword(s):  
Sperm Motility ◽  
Storage Temperature ◽  
Bufo Marinus ◽  
Cane Toad ◽  
Short Term ◽  
Optimal Protocol ◽  
Short Term Storage ◽  
Rare And Endangered ◽  
Toad Bufo ◽  
Term Storage

The responses of cane toad (Bufo marinus) gametes, used as a model for the development of assisted reproduction techniques for rare and endangered amphibians, to short-term storage at temperatures > 0 degrees C were studied. Whole excised testes were stored at 0 degrees or 4 degrees C for 15 days, and sperm motility was measured at excision and after storage for 2, 5, 7, 10, 12 and 15 days. Spermatozoa showed > 50% motility for 7 days at 0 degrees C and for 5 days at 4 degrees C. At 15 days, only spermatozoa stored at 0 degrees C still showed some motility (3%). Sperm suspensions were prepared at 5 day intervals over 30 days in simplified amphibian ringer (SAR) at dilutions of 1:1, 1:5 and 1:10 (w/v) testes:SAR. Aliquots from each dilution were stored at 0 degrees C in Eppendorf tubes opened at 5 day intervals of storage (aerated) or kept sealed (unaerated) (treatments: aerated or unaerated; 5, 10, 15, 20, 25 and 30 days storage). After 30 days, sperm motility and fertilizing capacity were determined. The optimal protocol for sperm storage up to 10 days, as assessed by the retention of fertilizing capacity, was as a 1:5 testis:SAR (w/v) suspension, whereas the longest absolute retention of both motility and fertilizing capacity was observed in concentrated (1:1 dilution), anaerobic suspensions (up to 25-30 days). Oviductal oocytes placed in SAR at 5, 10, 15, 20 and 25 degrees C immediately after ovulation lost viability when cooled rapidly to 5 degrees C and stored for 2 h. However, oocytes retained viability for up to 8 h at the optimum storage temperature of 15 degrees C. Thus, it is concluded that during short-term storage spermatozoa retain viability for longer than oocytes, and that spermatozoa in suspensions retain viability for longer than spermatozoa stored in situ in excised testes.

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