scholarly journals Transcriptomic Identification and Biochemical Characterization of HmpA, a Nitric Oxide Dioxygenase, Essential for Pathogenesis of Vibrio vulnificus

2019 ◽  
Vol 10 ◽  
Author(s):  
Dukyun Kim ◽  
Eun Jung Na ◽  
Suhyeon Kim ◽  
Jung Sung Kim ◽  
Young Hyun Jung ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Biochemical Characterization ◽  
Vibrio Vulnificus

scholarly journals Purification and biochemical characterization of FrsA protein from Vibrio vulnificus as an esterase

PLoS ONE ◽  
2019 ◽  
Vol 14 (4) ◽  
pp. e0215084
Author(s):  
Xiaoqin Wang ◽  
Zhi-Min Li ◽  
Qingyue Li ◽  
Mingsong Shi ◽  
Lingling Bao ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Vibrio Vulnificus

scholarly journals Physarum nitric oxide synthases: genomic structures and enzymology of recombinant proteins

2009 ◽  
Vol 418 (3) ◽  
pp. 691-700 ◽  
Author(s):  
Simon Messner ◽  
Stephan Leitner ◽  
Christian Bommassar ◽  
Georg Golderer ◽  
Peter Gröbner ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Recombinant Proteins ◽  
Functional Role ◽  
Genomic Organization ◽  
Biochemical Characterization ◽  
Recombinant Expression ◽  
Nitric Oxide Synthases ◽  
Biochemical Properties ◽  
Terminal Amino

Physarum polycephalum expresses two closely related, calcium-independent NOSs (nitric oxide synthases). In our previous work, we showed that both NOSs are induced during starvation and apparently play a functional role in sporulation. In the present study, we characterized the genomic structures of both Physarum NOSs, expressed both enzymes recombinantly in bacteria and characterized their biochemical properties. Whereas the overall genomic organization of Physarum NOS genes is comparable with various animal NOSs, none of the exon–intron boundaries are conserved. Recombinant expression of clones with various N-termini identified N-terminal amino acids essential for enzyme activity, but not required for haem binding or dimerization, and suggests the usage of non-AUG start codons for Physarum NOSs. Biochemical characterization of the two Physarum isoenzymes revealed different affinities for L-arginine, FMN and 6R-5,6,7,8-tetrahydro-L-biopterin.

scholarly journals Biochemical characterization of a catalase from Vibrio vulnificus, a pathogen that causes gastroenteritis

2017 ◽  
Vol 64 (3) ◽  
pp. 543-549 ◽  
Author(s):  
Jihua Pei ◽  
Haijun Wang ◽  
Limin Wu ◽  
Shenglong Xia ◽  
Changlong Xu ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Vibrio Vulnificus

Structural and Biochemical Characterization of the Curcumin-Reducing Activity of CurA from Vibrio vulnificus

2018 ◽  
Vol 66 (40) ◽  
pp. 10608-10616 ◽  
Author(s):  
Soo-Bong Park ◽  
Da-Woon Bae ◽  
Nina Abigail B. Clavio ◽  
Lei Zhao ◽  
Chang-Sook Jeong ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Vibrio Vulnificus ◽  
Reducing Activity
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