Biological control of Fusarium oxysporum f. sp. cubense Tropical Race 4 in banana plantlets using newly isolated Streptomyces sp. WHL7 from marine soft coral

Plant Disease ◽  
2021 ◽  
Author(s):  
Jie Wang ◽  
Bingyu Cai ◽  
Kai Li ◽  
Yankun Zhao ◽  
Chunyu Li ◽  
...  
Keyword(s):  
Biological Control ◽  
Fusarium Oxysporum ◽  
Soft Coral ◽  
Fermentation Broth ◽  
Cell Integrity ◽  
Streptomyces Sp ◽  
Promising Strategy ◽  
Almost All ◽  
Race 4 ◽  
High Antifungal Activity

Banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) is a disastrous fungal disease. Foc tropical race 4 (Foc TR4) infects almost all banana cultivars. Use of chemical fungicides caused seriously environment pollution. Biological control with antagonistic microbes is a promising strategy for controlling Foc TR4. Here, strain WHL7 isolated from marine soft coral exhibited a high antifungal activity against Foc TR4. Based on the morphological and physicochemical profiles as well as the phylogenetic tree, the strain was assigned to Streptomyces sp.. Fermentation broth of Streptomyces sp. WHL7 significantly increased the resistance of banana plantlets to Foc TR4 in the pot experiment. Analysis of antifungal mechanism showed that strain WHL7 extracts inhibited spore germination and mycelial growth of Foc TR4, and destroyed cell integrity and ultrastructure. Hence, Streptomyces sp. WHL7 is an important bioresource for exploring novel natural products and biofertilizer to manage Foc TR4.

scholarly journals Non-Synergistic Effect of Trichoderma harzianum and Glomus spp. in Reducing Infection of Fusarium Wilt in Banana

Pathogens ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 43 ◽  
Author(s):  
Arfe Castillo ◽  
Cecirly Puig ◽  
Christian Cumagun
Keyword(s):  
Biological Control ◽  
Synergistic Effect ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Trichoderma Harzianum ◽  
Root Weight ◽  
Biological Control Agents ◽  
Competitive Effects ◽  
Glomus Spp ◽  
Race 4

Philippine banana is currently threatened by Fusarium oxysporum f. sp. cubense Tropical Race 4 (FocR4). This study investigated the use of Trichoderma harzianum pre-treated with Glomus spp, as a means of managing Fusarium wilt on young ‘Lakatan’ banana seedlings. Results showed that Glomus applied basally significantly improved banana seedling growth with increased increment in plant height and pseudostem diameter and heavier root weight. The application of Glomus spp. alone offered 100% protection to the ‘Lakatan’ seedlings against FocR4 as indicated by the absence of the wilting symptom. A combination of T. harzianum and Glomus spp. also gave significant effect against Fusarium wilt through delayed disease progression in the seedlings but was not synergistic. Competitive effects were suspected when application of the two biological control agents on banana roots was done simultaneously.

scholarly journals Biological Management of Banana Fusarium Wilt Caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 Using Antagonistic Fungal Isolate CSR-T-3 (Trichoderma reesei)

2020 ◽  
Vol 11 ◽  
Author(s):  
Thukkaram Damodaran ◽  
Shailendra Rajan ◽  
Manoharan Muthukumar ◽  
Ram Gopal ◽  
Kavita Yadav ◽  
...  
Keyword(s):  
Gene Expression ◽  
Biological Control ◽  
Fusarium Oxysporum ◽  
Trichoderma Reesei ◽  
Disease Severity ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Severity Index ◽  
Disease Severity Index ◽  
Race 4

Fusarium wilt in bananas is one of the most devastating diseases that poses a serious threat to the banana industry globally. With no effective control measures available to date, biological control has been explored to restrict the spread and manage the outbreak. We studied the effective biological control potential of different Trichoderma spp. in the management of Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4). Expression of the defense related genes and metabolites in banana plants inoculated with Foc TR4 and treated with effective Trichoderma sp interactions were also studied. The in vitro growth inhibition of Foc TR4 by Trichoderma reesei isolate CSR-T-3 was 85.19% indicating a higher antagonistic potential than other Trichoderma isolates used in the study. Further, in in vivo assays, the banana plants treated with the isolate CSR-T-3 T. reesei had a significant reduction in the disease severity index (0.75) and also had increased phenological indices with respect to Foc TR4 treated plants. Enhanced activity of defense enzymes, such as β-1, 3-glucanase, peroxidase, chitinase, polyphenol oxidase, and phenylalanine ammonia lyase with higher phenol contents were found in the Trichoderma isolate CSR-T-3 treated banana plants challenge-inoculated with Foc TR4. Fusarium toxins, such as fusaristatin A, fusarin C, chlamydosporal, and beauveric acid were identified by LC-MS in Foc TR4-infected banana plants while high intensity production of antifungal compounds, such as ß-caryophyllene, catechin-o-gallate, soyasapogenol rhamnosyl glucoronide, peptaibols, fenigycin, iturin C19, anthocyanin, and gallocatechin-o-gallate were detected in T. reesei isolate CSR-T-3 treated plants previously inoculated with Foc TR4. Gene expression analysis indicated the upregulation of TrCBH1/TrCBH2, TrXYL1, TrEGL1, TrTMK1, TrTGA1, and TrVEL1 genes in CSR-T-3 treatment. LC-MS and gene expression analysis could ascertain the upregulation of genes involved in mycoparasitism and the signal transduction pathway leading to secondary metabolite production under CSR-T-3 treatment. The plants in the field study showed a reduced disease severity index (1.14) with high phenological growth and yield indices when treated with T. reesei isolate CSR-T-3 formulation. We report here an effective biocontrol-based management technological transformation from lab to the field for successful control of Fusarium wilt disease caused by Foc TR4 in bananas.

Effects of Fusarium oxysporum f. sp. cubense race 4 on microorganisms and enzyme activities in the rhizosphere soil of banana

2013 ◽  
Vol 38 (2) ◽  
pp. 173-176 ◽  
Author(s):  
Yong-hong HUANG ◽  
Shun LÜ ◽  
Chun-yu LI ◽  
Yue-rong WEI ◽  
Gan-jun YI
Keyword(s):  
Fusarium Oxysporum ◽  
Enzyme Activities ◽  
Rhizosphere Soil ◽  
Race 4

Biological control of Fusarium wilt of cucumber with nonpathogenic isolates of Fusarium oxysporum

1987 ◽  
Vol 33 (5) ◽  
pp. 349-353 ◽  
Author(s):  
T. C. Paulitz ◽  
C. S. Park ◽  
R. Baker
Keyword(s):  
Biological Control ◽  
Fusarium Oxysporum ◽  
Infection Rate ◽  
Fusarium Wilt ◽  
Disease Incidence ◽  
Infection Rates ◽  
Control Activity ◽  
Significant Difference ◽  
Cucumber Roots ◽  
Linear Regressions

Nonpathogenic isolates of Fusarium oxysporum were obtained from surface-disinfested, symptomless cucumber roots grown in two raw (nonautoclaved) soils. These isolates were screened for pathogenicity and biological control activity against Fusarium wilt of cucumber in raw soil infested with Fusarium oxysporum f. sp. cucumerinum (F.o.c.). The influence of three isolates effective in inducing suppressiveness and three ineffective isolates on disease incidence over time was tested. The effective isolates reduced the infection rate (R), based on linear regressions of data transformed to loge (1/1 – y). Effective isolate C5 was added to raw soil infested with various inoculum densities of F.o.c. In treatments without C5, the increase in inoculum densities of F.o.c. decreased the incubation period of wilt disease, but there was no significant difference in infection rate among the inoculum density treatments. Isolate C5 reduced the infection rate at all inoculum densities of F.o.c. Various inoculum densities of C5 were added to raw soils infested with 1000 cfu/g of F.o.c. In the first trial, infection rates were reduced only in the treatment with 10 000 cfu/g of C5; in the second trial, infection rates were reduced in treatments with 10 000 and 30 000 cfu/g of C5.

scholarly journals An Actinobacterial Isolate, Streptomyces sp. YX44, Produces Broad-Spectrum Antibiotics That Strongly Inhibit Staphylococcus aureus

2021 ◽  
Vol 9 (3) ◽  
pp. 630
Author(s):  
Tien-Lin Chang ◽  
Tzu-Wen Huang ◽  
Ying-Xuan Wang ◽  
Chang-Pan Liu ◽  
Ralph Kirby ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Fermentation Broth ◽  
Multidrug Resistant ◽  
Size Exclusion ◽  
Significant Activity ◽  
Health Food ◽  
Strong Activity ◽  
Genus Streptomyces ◽  
Streptomyces Sp ◽  
Good Solubility

The need for new antibiotics is increasing due to their overuse, and antibiotic resistance has become one of the major threats worldwide to public health, food safety, and clinical treatment. In this study, we describe an actinobacterial isolate, YX44, which belongs to the genus Streptomyces. This Streptomyces was isolated from a drinking pipe located in Osaka, Japan, and has the ability to inhibit Gram-positive bacteria, Gram-negative bacteria, and various fungi. YX44 fermentation broth shows strong activity against Escherichia coli and Staphylococcus aureus, as well as also inhibiting clinical isolates of multidrug-resistant Staphylococcus aureus. The YX44 antibacterial substances in the broth are relatively heat-stable, show high stability from the pH range 1 to 11, and have good solubility in both organic and non-organic solvents. Size-exclusion chromatography revealed that the YX44 antibacterial compounds are less than 1000 Da in size. LC-MS was able to identify three possible candidate molecules with molecular weights of 308, 365, 460, and 653 g/mol; none of these sizes correspond to any well-known antibiotics. Our results show that Streptomyces sp. YX44 seems to produce a number of novel antibiotics with high pH stability and good solubility that have significant activity against S. aureus, including multidrug-resistant strains.

The Long horned beetles ( Cerambycidae: Coleoptera) with new records from Pakistan

2021 ◽  
Vol 27 (1) ◽  
pp. 79-91
Author(s):  
Waheed Ali Panhwar ◽  
Kamran Ahmed Pathan ◽  
Abdul Manan Shaikh ◽  
Safdar Ali Ujjan ◽  
Javed Ahmed Ujan ◽  
...  
Keyword(s):  
Biological Control ◽  
New Record ◽  
New Records ◽  
Agricultural Fields ◽  
Polar Regions ◽  
Biological Control Agents ◽  
Longhorn Beetles ◽  
Almost All ◽  
Horned Beetles

The longhorn beetles belongs to family Cerambycidae. The beetles are present in almost all ecosystems, except the ocean and Polar Regions. They are most the important biological control agents into agro-ecosystems. The beetles help in the biological control they eat extensive assortments of tree dwelling, soil dwelling insects and also eat caterpillars, maggots, aphids, bug, ants, wasp. Long horned beetles were gathered from different sites (agricultural fields and their surrounding vegetation) of district Naushahro Feroze with insect net (7.79 cm in diameter and 49.9 cm in length) and hand picking. A of 234 specimens were captured from October 2018 to July 2019. The material was identified into 07 species out of 06 genera. Of which Batocera rubus (Linnaeus, 1758), New Record from Sindh, Batocera rufomaculata (Charles De Geer, 1775), New Record from Sindh, Apriona cinerea (Chevrolat, 1852), New Record from Sindh, Archopalus exoticus (Sharp, 1905), New Record from Pakistan Macrotoma crenata (Fabricius, 1801), New Record from Pakistan, Prionus corpulantus (Bates, 1878) New Record from Sindh, Dorysthenes hugelii (Redtenbacher, 1848), New Record from Pakistan. The highest ratio of specimens were recorded from Mehrabpur and lowest ratio of specimens were recorded from Moro.

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