scholarly journals Visualizing Microorganism-Mineral Interaction in the Iberian Pyrite Belt Subsurface: The Acidovorax Case

2020 ◽  
Vol 11 ◽  
Author(s):  
Cristina Escudero ◽  
Adolfo del Campo ◽  
Jose R. Ares ◽  
Carlos Sánchez ◽  
Jose M. Martínez ◽  
...  
Keyword(s):  
Strong Correlation ◽  
Extreme Environment ◽  
Iberian Pyrite Belt ◽  
Raman Microscopy ◽  
Deep Subsurface ◽  
Rock Samples ◽  
Mineral Interaction

Despite being considered an extreme environment, several studies have shown that life in the deep subsurface is abundant and diverse. Microorganisms inhabiting these systems live within the rock pores and, therefore, the geochemical and geohydrological characteristics of this matrix may influence the distribution of underground biodiversity. In this study, correlative fluorescence and Raman microscopy (Raman-FISH) was used to analyze the mineralogy associated with the presence of members of the genus Acidovorax, an iron oxidizing microorganisms, in native rock samples of the Iberian Pyrite Belt subsurface. Our results suggest a strong correlation between the presence of Acidovorax genus and pyrite, suggesting that the mineral might greatly influence its subsurface distribution.

scholarly journals Viable cyanobacteria in the deep continental subsurface

2018 ◽  
Vol 115 (42) ◽  
pp. 10702-10707 ◽  
Author(s):  
Fernando Puente-Sánchez ◽  
Alejandro Arce-Rodríguez ◽  
Monike Oggerin ◽  
Miriam García-Villadangos ◽  
Mercedes Moreno-Paz ◽  
...  
Keyword(s):  
Ecological Niche ◽  
Iberian Pyrite Belt ◽  
Primary Producers ◽  
Deep Subsurface ◽  
Marine Systems ◽  
Origin And Evolution ◽  
Rock Samples ◽  
Deep Earth ◽  
Mars Analog ◽  
Ecological Range

Cyanobacteria are ecologically versatile microorganisms inhabiting most environments, ranging from marine systems to arid deserts. Although they possess several pathways for light-independent energy generation, until now their ecological range appeared to be restricted to environments with at least occasional exposure to sunlight. Here we present molecular, microscopic, and metagenomic evidence that cyanobacteria predominate in deep subsurface rock samples from the Iberian Pyrite Belt Mars analog (southwestern Spain). Metagenomics showed the potential for a hydrogen-based lithoautotrophic cyanobacterial metabolism. Collectively, our results suggest that they may play an important role as primary producers within the deep-Earth biosphere. Our description of this previously unknown ecological niche for cyanobacteria paves the way for models on their origin and evolution, as well as on their potential presence in current or primitive biospheres in other planetary bodies, and on the extant, primitive, and putative extraterrestrial biospheres.

scholarly journals Draft Genome Sequence of Rhodoplanes sp. Strain T2.26MG-98, Isolated from 492.6 Meters Deep on the Subsurface of the Iberian Pyrite Belt

2019 ◽  
Vol 8 (16) ◽  
Author(s):  
N. Mariñán ◽  
José M. Martínez ◽  
T. Leandro ◽  
R. Amils
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Iberian Pyrite Belt ◽  
Draft Genome Sequence ◽  
Deep Subsurface

Rhodoplanes sp. strain T2.26MG-98 was isolated from the deep subsurface of the Iberian Pyrite Belt. We report its draft genome, consisting of 214 contigs with a chromosome of ∼5.6 Mb and a 53.7-kb plasmid.

Deep subsurface sulfate reduction and methanogenesis in the Iberian Pyrite Belt revealed through geochemistry and molecular biomarkers

Geobiology ◽  
2013 ◽  
Vol 12 (1) ◽  
pp. 34-47 ◽  
Author(s):  
F. Puente-Sánchez ◽  
M. Moreno-Paz ◽  
L. A. Rivas ◽  
P. Cruz-Gil ◽  
M. García-Villadangos ◽  
...  
Keyword(s):  
Sulfate Reduction ◽  
Iberian Pyrite Belt ◽  
Molecular Biomarkers ◽  
Deep Subsurface

scholarly journals Draft Genome Sequence of the Deep-Subsurface Actinobacterium Tessaracoccus lapidicaptus IPBSL-7 T

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Fernando Puente-Sánchez ◽  
Dietmar H. Pieper ◽  
Alejandro Arce-Rodríguez
Keyword(s):  
Genome Size ◽  
Genome Sequence ◽  
Dna Sequences ◽  
Type Strain ◽  
Draft Genome ◽  
Iberian Pyrite Belt ◽  
Draft Genome Sequence ◽  
Rrna Genes ◽  
Trna Genes ◽  
Deep Subsurface

The type strain of Tessaracoccus lapidicaptus was isolated from the deep subsurface of the Iberian Pyrite Belt (southwest Spain). Here, we report its draft genome, consisting of 27 contigs with a ~3.1-Mb genome size. The annotation revealed 2,905 coding DNA sequences, 45 tRNA genes, and three rRNA genes.

scholarly journals Draft Genome Sequence of Rhizobium sp. Strain T2.30D-1.1, Isolated from 538.5 Meters Deep on the Subsurface of the Iberian Pyrite Belt

2018 ◽  
Vol 7 (16) ◽  
Author(s):  
R. García ◽  
José M. Martínez ◽  
T. Leandro ◽  
R. Amils
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Iberian Pyrite Belt ◽  
Draft Genome Sequence ◽  
Deep Subsurface ◽  
Rhizobium Sp

Rhizobium sp. strain T2.30D-1.1 was isolated from the deep subsurface of the Iberian Pyrite Belt.

High Copper Tolerant P. lilacinum Strain Isolated from a Rich Environment in Copper, Río Tinto (Sw, Spain)

2015 ◽  
Vol 1130 ◽  
pp. 157-160
Author(s):  
Monike Oggerin ◽  
Cristina Escudero ◽  
Catalina del Moral ◽  
Nuria Rodríguez ◽  
Ricardo Amils
Keyword(s):  
Dry Weight ◽  
Extreme Environment ◽  
Iberian Pyrite Belt ◽  
Resistance Mechanisms ◽  
Fungal Strain ◽  
Fungal Cell ◽  
Sw Spain ◽  
Purpureocillium Lilacinum ◽  
Proteomic Study ◽  
Copper Tolerant

Río Tinto (Southwestern Spain, Iberian Pyrite Belt) is an unusual extreme environment with an unexpected level of eukaryotic microbial diversity. Of the different fungal strains isolated along the river over several years, one of them,Purpureocillium lilacinumM15001, is able to tolerate up to 1M Cu concentrations. This strain was able to remove 25% of the Cu when incubated at 50mM, 32% at 100mM and 51% at 500mM Cu concentrations. The amount of Cu detected inside the cells increased accordingly the concentration of Cu in the solution, from 8.2% of the total fungal dry weight at 50mM Cu concentration, to 13% when exposed to 100mM Cu and 22.4% at 500mM Cu. TEM studies showed the existence of electrodense material externally adsorbed to the fungal cell wall, as well as large intracellular deposits. EDX microanalyses revealed that this material was composed mainly of Cu. To clarify the possible resistance mechanisms involved in the tolerance of this fungal strain to Cu, a proteomic study has been carried out.

scholarly journals Draft Genome Sequence of Shewanella sp. Strain T2.3D-1.1, Isolated from 121.8 Meters Deep in the Subsurface of the Iberian Pyrite Belt

2020 ◽  
Vol 9 (40) ◽  
Author(s):  
Sofia de Francisco-Polanco ◽  
José M. Martínez ◽  
Tania Leandro ◽  
Ricardo Amils
Keyword(s):  
Genome Sequence ◽  
Dna Sequences ◽  
Draft Genome ◽  
Iberian Pyrite Belt ◽  
Rrna Operon ◽  
Draft Genome Sequence ◽  
Trna Genes ◽  
Deep Subsurface

ABSTRACT Shewanella sp. strain T2.3D-1.1 was isolated from the deep subsurface of the Iberian Pyrite Belt. We report its draft genome sequence, consisting of 49 scaffolds, with a chromosome of ≈4.6 Mb and a 23.8-kb plasmid. The chromosome annotation identified 4,068 coding DNA sequences, 1 rRNA operon, and 108 tRNA genes.

scholarly journals Biological Production of H 2 , CH 4 , and CO 2 in the Deep Subsurface of the Iberian Pyrite Belt

2021 ◽  
Author(s):  
Jose L. Sanz ◽  
Nuria Rodriguez ◽  
Cristina Escudero ◽  
Daniel Carrizo ◽  
Ricardo Amils
Keyword(s):  
Iberian Pyrite Belt ◽  
Deep Subsurface ◽  
Biological Production

Cultural and Linguistic Adaptation of the Consensus Auditory-Perceptual Evaluation of Voice (CAPE-V) Into Hindi

2020 ◽  
Vol 63 (12) ◽  
pp. 3974-3981
Author(s):  
Ashwini Joshi ◽  
Isha Baheti ◽  
Vrushali Angadi
Keyword(s):  
Strong Correlation ◽  
Concurrent Validity ◽  
Interrater Reliability ◽  
Voice Quality ◽  
Weak Correlation ◽  
Voice Assessment ◽  
Perceptual Evaluation ◽  
Severity Grade ◽  
Normal Voice ◽  
Group A

Aim The purpose of this study was to develop and assess the reliability of a Hindi version of the Consensus Auditory-Perceptual Evaluation of Voice (CAPE-V). Reliability was assessed by comparing Hindi CAPE-V ratings with English CAPE-V ratings and by the Grade, Roughness, Breathiness, Asthenia and Strain (GRBAS) scale. Method Hindi sentences were created to match the phonemic load of the corresponding English CAPE-V sentences. The Hindi sentences were adapted for linguistic content. The original English and adapted Hindi CAPE-V and GRBAS were completed for 33 bilingual individuals with normal voice quality. Additionally, the Hindi CAPE-V and GRBAS were completed for 13 Hindi speakers with disordered voice quality. The agreement of CAPE-V ratings was assessed between language versions, GRBAS ratings, and two rater pairs (three raters in total). Pearson product–moment correlation was completed for all comparisons. Results A strong correlation ( r > .8, p < .01) was found between the Hindi CAPE-V scores and the English CAPE-V scores for most variables in normal voice participants. A weak correlation was found for the variable of strain ( r < .2, p = .400) in the normative group. A strong correlation ( r > .6, p < .01) was found between the overall severity/grade, roughness, and breathiness scores in the GRBAS scale and the CAPE-V scale in normal and disordered voice samples. Significant interrater reliability ( r > .75) was present in overall severity and breathiness. Conclusions The Hindi version of the CAPE-V demonstrates good interrater reliability and concurrent validity with the English CAPE-V and the GRBAS. The Hindi CAPE-V can be used for the auditory-perceptual voice assessment of Hindi speakers.

A hierarchical model of team climate in an extreme environment

2003 ◽  
Author(s):  
Lacey L. Schmidt ◽  
JoAnna Wood ◽  
Peter Sullivan
Keyword(s):  
Hierarchical Model ◽  
Extreme Environment ◽  
Team Climate
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