scholarly journals Isolation and Characterization of Enterococcus faecalis-Infecting Bacteriophages From Different Cheese Types

2021 ◽  
Vol 11 ◽  
Author(s):  
Beatriz del Rio ◽  
Esther Sánchez-Llana ◽  
Noelia Martínez ◽  
María Fernández ◽  
Victor Ladero ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Fermented Foods ◽  
Isolation And Characterization ◽  
Different Types ◽  
Clinical Environments ◽  
Genomic Characterization ◽  
Diverse Origin ◽  
Hospital Acquired ◽  
Silver Bullet

Enterococci are a diverse group of Gram-positive, lactic acid bacteria (LAB). They are found in many environments, including fermented foods, in which they could constitute a health threat since they produce biogenic amines, which consumption can lead to intoxication. Moreover, enterococci has also emerged as an important hospital-acquired pathogens via its acquisition of antimicrobial resistance. Bacteriophages possess features that make them optimal biotechnological weapons for controlling bacterial growth in disease and food spoilage contexts. However, no silver bullet bacteriophage exists that can eliminate all the undesirable bacteria in a complex environment. Rather, a combination of phages with different host ranges would be required which implies the need for large collections of diverse phages. This work reports the isolation of several Enterococcus faecalis-infecting bacteriophages from different types of cheese, along with the range of E. faecalis strains of diverse origin (from dairy to clinical environments) they are able to infect. The isolated phages showed a large diversity regarding the number and origin of strains they infect. Some of these phages were subjected to morphological and genomic characterization which confirmed their diversity and showed they belong to different families and genera. The present findings increase the potential arsenal for the bacteriophage-based biocontrol of harmful E. faecalis populations.

Isolation and Characterization of Enterocin SE-K4 Produced by Thermophilic Enterococci, Enterococcus faecalis K-4

2001 ◽  
Vol 65 (2) ◽  
pp. 247-253 ◽  
Author(s):  
Tomoko EGUCHI ◽  
Kazuyo KAMINAKA ◽  
Jun SHIMA ◽  
Shinichi KAWAMOTO ◽  
Katsumi MORI ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Isolation And Characterization

Isolation and Characterization of Lactic Acid Bacteria from Fermented Foods

2013 ◽  
Vol 26 (2) ◽  
pp. 325 ◽  
Author(s):  
Sangita Bansal ◽  
Apoorva Singh ◽  
Manisha Mangal ◽  
Satish K Sharma
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Fermented Foods ◽  
Isolation And Characterization

Isolation and characterization of lactobacilli from human faeces and indigenous fermented foods for their potential application as probiotics

2016 ◽  
Vol 62 (4) ◽  
pp. 349-359 ◽  
Author(s):  
Hemanti Mandal ◽  
Ruchi Jariwala ◽  
Tamishraha Bagchi
Keyword(s):  
Bile Salt ◽  
Lactobacillus Rhamnosus ◽  
Antagonistic Activity ◽  
Food Sources ◽  
Fermented Foods ◽  
E Coli ◽  
Isolation And Characterization ◽  
Acid And Bile Tolerance ◽  
Ht 29

This study was conducted to select Lactobacillus strains from various sources on the basis of their probiotic attributes, such as acid and bile tolerance, binding to intestinal cells, and antimicrobial activity. Twelve isolates were obtained from human and food sources and were evaluated against standard probiotic Lactobacillus rhamnosus GG (LGG). Isolates were also studied for their antibiotic susceptibility. Isolate Lactobacillus fermentum GPI-6 showed the best survival profile at 0.3% and 1% bile salt, as compared with LGG. Isolates Lactobacillus plantarum GRI-2 and Lactobacillus salivarius GPI-4 showed no reduction in survival rate at pH 2.5. As expected, isolates showed strain-specific differences when comparing various attributes. Isolates GPI-4, GPI-7, and FA-5 showed better adhesion to HT-29, while isolate GPI-4 adhered better to Caco-2 cells than did LGG. However, when studying their ability to compete with Escherichia coli O26:H11, isolates GPI-6 and GPI-7 significantly inhibited E. coli adhesion to both HT-29 and Caco-2 cells compared with LGG. In conclusion, isolates GPI-4, GPI-7, and FA-5 showed excellent binding ability and antagonistic activity and better tolerance to acidic pH (pH 2.5) and to different bile salt concentrations in comparison with LGG, and hence, they could be considered as potential probiotic candidates.

Isolation and characterization of genetic expression and secretion signals from Enterococcus faecalis through the use of broad-host-range α-amylase probe vectors

Gene ◽  
1992 ◽  
Vol 118 (1) ◽  
pp. 21-30 ◽  
Author(s):  
Pascal Hols ◽  
Alain Baulard ◽  
Dominique Garmyn ◽  
Brigitte Delplace ◽  
Stéphane Hogan ◽  
...  
Keyword(s):  
Host Range ◽  
Enterococcus Faecalis ◽  
Broad Host Range ◽  
Genetic Expression ◽  
Isolation And Characterization

scholarly journals Thai Fermented Foods as a Versatile Source of Bioactive Microorganisms—A Comprehensive Review

2018 ◽  
Vol 86 (3) ◽  
pp. 37 ◽  
Author(s):  
Bhagavathi Sivamaruthi ◽  
Periyanaina Kesika ◽  
Chaiyavat Chaiyasut
Keyword(s):  
Fermented Food ◽  
Bioactive Molecules ◽  
Fermented Foods ◽  
Spontaneous Fermentation ◽  
Isolation And Characterization ◽  
Probiotic Microbes ◽  
Asian People ◽  
Traditional Fermented Foods

Fermented foods are known for several health benefits, and they are generally used among the Asian people. Microorganisms involved in the fermentation process are most responsible for the final quality of the food. Traditional fermented (spontaneous fermentation) foods are a versatile source of bioactive molecules and bioactive microbes. Several reports are available regarding the isolation and characterization of potent strains from traditional fermented foods. A collection of information for easy literature analysis of bioactive microbes derived from Thai fermented food is not yet available. The current manuscript compiled information on bioactive (antimicrobial- and enzyme-producing probiotic) microbes isolated from naturally fermented Thai foods.

Isolation and Characterization of a Novel Bacteriophage φ4D Lytic Against Enterococcus faecalis Strains

2012 ◽  
Vol 65 (3) ◽  
pp. 284-289 ◽  
Author(s):  
Sylwia Parasion ◽  
Magdalena Kwiatek ◽  
Lidia Mizak ◽  
Romuald Gryko ◽  
Michał Bartoszcze ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Isolation And Characterization

scholarly journals Genomic Characterization of MDR Escherichia coli Harboring blaOXA-48 on the IncL/M-type Plasmid Isolated from Blood Stream Infection

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
S. Alousi ◽  
T. Salloum ◽  
H. Arabaghian ◽  
G. M. Matar ◽  
G. F. Araj ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Blood Stream Infection ◽  
Genomic Analysis ◽  
Blood Stream ◽  
Population Movements ◽  
E Coli ◽  
Resistance Determinants ◽  
Genomic Characterization ◽  
Hospital Acquired

Escherichia coli is responsible for a wide variety of community and hospital acquired extraintestinal infections, and the emergence of ESBL resistant isolates is a major clinical concern. In this study, we characterized the genomic attributes of an OXA-48 and CTX-M-3 producing E. coli EC-IMP153. Whole-genome initial assembly produced 146 contigs with a combined 5,504,170 bp in size and a G+C content of 50.5%. wgSNPs-based phylogenetic comparison with 36 publically available genomes was also performed. Comprehensive genomic analysis showed that EC-IMP153 belonged to sequence type ST-405 and harbored several resistance determinants including the β-lactam resistance genes blaOXA-48, blaCTX-M-3, blaTEM-1B, blaOXA-1, and blaCMY-70, aminoglycoside fyuA and aac(3)IId, tetracycline tet(A) and tet(R), and fluoroquinolone gyrA, parC, and mfd resistance determinants. Plasmids with the following incompatibility groups were detected in silico and confirmed using PBRT: IncI1-α, IncL, IncW, Col (BS512), and IncF. To our knowledge this is the first in-depth genomic analysis of an OXA-48 producing E. coli ST-405 isolated from a patient in Lebanon and linked to a blood stream infection. Continuous monitoring is necessary to better understand the continued diffusion of such pathogens, especially in view of the population movements triggered by unrest in the Middle East.

scholarly journals Isolation and characterization of halotolerant killer yeasts from fermented foods.

1989 ◽  
Vol 53 (10) ◽  
pp. 2593-2597 ◽  
Author(s):  
Chise SUZUKI ◽  
Kazufumi YAMADA ◽  
Noriyuki OKADA ◽  
Sayuki NIKKUNI
Keyword(s):  
Fermented Foods ◽  
Isolation And Characterization ◽  
Killer Yeasts
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