scholarly journals Enhanced Reactive Blue 4 Biodegradation Performance of Newly Isolated white rot fungus Antrodia P5 by the Synergistic Effect of Herbal Extraction Residue

2021 ◽  
Vol 12 ◽  
Author(s):  
Tianjie Yuan ◽  
Shuyi Zhang ◽  
Yifei Chen ◽  
Ran Zhang ◽  
Letian Chen ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Lignin Peroxidase ◽  
Dye Decolorization ◽  
White Rot ◽  
White Rot Fungus ◽  
Fungal Treatment ◽  
Maximum Increase ◽  
Anthraquinone Dye ◽  
Wide Range ◽  
Reactive Blue 4

In this study, a white rot fungus Antrodia was newly isolated and named P5. Then its dye biodegradation ability was investigated. Our results showed that P5 could effectively degrade 1,000 mg/L Reactive Blue 4 (RB4) in 24 h with 95% decolorization under shaking conditions. It could tolerate a high dye concentration of 2,500 mg/L as well as 10% salt concentration and a wide range of pH values (4–9). Herbal extraction residues (HER) were screened as additional medium elements for P5 biodegradation. Following the addition of Fructus Gardeniae (FG) extraction residue, the biodegradation performance of P5 was significantly enhanced, achieving 92% decolorization in 12 h. Transcriptome analysis showed that the expression of multiple peroxidase genes was simultaneously increased: Lignin Peroxidase, Manganese Peroxidase, Laccase, and Dye Decolorization Peroxidase. The maximum increase in Lignin Peroxidase reached 10.22-fold in the presence of FG. The results of UV scanning and LC-HRMS showed that with the synergistic effect of FG, P5 could remarkably accelerate the biodegradation process of RB4 intermediates. Moreover, the fungal treatment with FG also promoted the abatement of RB4 toxicity. In sum, white rot fungus and herbal extraction residue were combined and used in the treatment of anthraquinone dye. This could be applied in practical contexts to realize an efficient and eco-friendly strategy for industrial dye wastewater treatment.

The Onset of Lignin-Modifying Enzymes, Decrease of AOX and Color Removal by White-Rot Fungi Grown on Bleach Plant Effluents

1991 ◽  
Vol 24 (3-4) ◽  
pp. 189-198 ◽  
Author(s):  
V. P. Lankinen ◽  
M. M. Inkeröinen ◽  
J. Pellinen ◽  
A. I. Hatakka
Keyword(s):  
Lignin Peroxidase ◽  
Active Form ◽  
White Rot Fungi ◽  
Pulp Mill ◽  
Color Removal ◽  
Effluent Treatment ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignin Peroxidases ◽  
Pulp Mill Effluents

Decrease of adsorbable organic chlorine (AOX) is becoming the most important criterion for the efficiency of pulp mill effluent treatment in the 1990s. Two methods, designated MYCOR and MYCOPOR which utilize the white-rot fungus Phanerochaete chrysosporium have earlier been developed for the color removal of pulp mill effluents, but the processes have also a capacity to decrease the amount of chlorinated organic compounds. Lignin peroxidases (ligninases) produced by P. chrvsosporium may dechlorinate chlorinated phenols. In this work possibilities to use selected white-rot fungi in the treatment of E1-stage bleach plant effluent were studied. Phlebia radiata. Phanerochaete chrvsosporium and Merulius (Phlebia) tremellosus were compared in shake flasks for their ability to produce laccase, lignin peroxidase(s) and manganese-dependent peroxidase(s) and to remove color from a medium containing effluent. Softwood bleaching effluents were treated by carrier-immobilized P. radiata in 2 1 bioreactors and a 10 1 BiostatR -fermentor. Dechlorination was followed using Cl ion and AOX determinations. All fungi removed the color of the effluent. In P. radiata cultivations AOX decrease was ca. 4 mg l−1 in one day. Apparent lignin peroxidase activities as determined by veratryl alcohol oxidation method were negligible or zero in a medium with AOX content of ca. 60 mg l−1, prepared using about 20 % (v/v) of softwood effluent. However, the purification of extracellular enzymes implied that large amounts of lignin peroxidases were present in the medium and, after the purification, in active form. Enzyme proteins were separated using anion exchange chromatography, and they were further characterized by electrophoresis (SDS-PAGE) to reveal the kind of enzymes that were present during AOX decrease and color removal. The most characteristic lignin peroxidase isoenzymes in effluent media were LiP2 and LiP3.

scholarly journals Identification of white rot fungus CP9 and its potential application in biopulping

2018 ◽  
Vol 14 (4) ◽  
pp. 721-726
Author(s):  
Nguyen Thi Hong Lien ◽  
Nguyen Van Hieu ◽  
Luong Thi Hong ◽  
Hy Tuan Anh ◽  
Phan Thi Hong Thao
Keyword(s):  
Rice Straw ◽  
Lignin Peroxidase ◽  
Paper Industry ◽  
Morphological Characteristics ◽  
White Rot Fungi ◽  
White Rot ◽  
White Rot Fungus ◽  
Wood Block ◽  
New Production ◽  
Cellulosic Fibers

Wood-rotting fungi represent an important component of forest ecosystems. Among them, white-rot fungi are the most efficient lignin degraders. Biopulping using white-rot fungi in pretreatment of the materials, is one of the solutions to overcome disadvantages of traditional production methods. Today, the isolation and screening of lignin degrading fungi capable for application in biopulping are of keen interest in Vietnam. The use of non–wood, plant fibres in pulp and paper industry, special, agricultural residuces such as rice and wheat straw, sugarcane baggase, cornstalks etc is the new production toward, potential, serving sustainable development. The fungus CP9, which possessed high ligninolytic activity, was identified and studied in pretreatment of rice straw for biopulping. The fruiting bodies of strain CP9 were effuse on trunk. The hymenium was porous and brown white with short tubes, the white mycelia penetrated wood block. The colony was off-white, blossom, irregularly circular. The mycelia were thick and closely bound together. Beside lignin, this fungus could degrade other substrates such as casein, carboxymethyl cellulose and starch. Biological and morphological characteristics of the fungus CP9 suggested its placement in subdivision Basidiomycota. Combined with the results of phylogenetic analysis, which showed 99% similarity of the fungus with species Leiotrametes lactinea, our strain was named as Leiotrametes lactinea CP9. This fungus could grow well on rice straw under solid state fermentation. Pretreatment of rice straw using L. lactinea CP9 was based on the activity of fungal lignin peroxidase and laccase. After 20 days, the residual enzyme activity was of 21.6 and 18.4 nkat/g material for lignin peroxidase and laccase, respectively. Pretreatment significantly improved the quality of straw, as lignin loss of 38% while cellulosic fibers were comparatively well preserved.

An extracellular yellow laccase from white rot fungus Trametes sp. F1635 and its mediator systems for dye decolorization

Biochimie ◽  
2018 ◽  
Vol 148 ◽  
pp. 46-54 ◽  
Author(s):  
Shou-Nan Wang ◽  
Qing-Jun Chen ◽  
Meng-Juan Zhu ◽  
Fei-Yang Xue ◽  
Wei-Cong Li ◽  
...  
Keyword(s):  
Dye Decolorization ◽  
White Rot ◽  
White Rot Fungus ◽  
Mediator Systems ◽  
Yellow Laccase

scholarly journals Influence of incubation time on the vibration and mechanic properties of mycowood

Holzforschung ◽  
2016 ◽  
Vol 70 (6) ◽  
pp. 557-565 ◽  
Author(s):  
Marjan Sedighi Gilani ◽  
Jürg Neuenschwander ◽  
Markus Heeb ◽  
Roman Furrer ◽  
Sergio J. Sanabria ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Sound Velocity ◽  
Treatment Time ◽  
Characteristic Impedance ◽  
Weight Ratio ◽  
Physical And Mechanical Properties ◽  
Longitudinal Direction ◽  
White Rot ◽  
White Rot Fungus ◽  
Fungal Treatment

Abstract The goal of the current study was to investigate the physical and mechanical properties of mycowood as a high quality tone-wood, obtained from Norway spruce by treatment of the white rot fungus Physisporinus vitreus as a function of the treatment time. In focus was the stiffness to weight ratio, which is often considered a main criterion for tone-wood selection. The vibro-mechanical properties were tested by non-destructive methods. The change of color and density were also measured after 4–12 months of fungal incubation. Density decreased up to 5% after 12 months of fungal treatment. Sound velocity was measured in small size specimens by means of the free-free vibration approach, while in large specimens the air-coupled ultrasound method was applied. The two techniques gave similar results and indicated that the sound velocity decreased in mycowood. Internal damping was increased in mycowood to a higher extent than the reduction in the specific modulus of elasticity (E/ρ) and thus the sound velocity in the material. The sound velocity was decreasing with increasing incubation times and scattering of data with this regard was larger in the transversal than in the longitudinal direction. The sound radiation coefficient and the characteristic impedance were enhanced in mycowood and its color was more brownish and richer in tone.

scholarly journals The Mechanism of Degradation of Alizarin Red by a White-Rot Fungus (Lenzites Gibbosa)

2021 ◽  
Author(s):  
Jian Zhang ◽  
YuJie Chi ◽  
Lianrong Feng
Keyword(s):  
Metabolic Pathway ◽  
Intermediate Product ◽  
Degradation Process ◽  
White Rot ◽  
White Rot Fungus ◽  
Alizarin Red ◽  
Anthraquinone Dye ◽  
Key Enzymes ◽  
Important Intermediate ◽  
Laccase Enzyme

Abstract Background Alizarin red (AR) is a typical anthraquinone dye, and the resulting wastewater is toxic and difficult to remove. A study showed that the white rot fungus Lenzites gibbosa (L. gibbosa) can degrade dye wastewater by decolorization and has evolved its own enzyme-producing traits. Methods In this study, transcriptome sequencing was performed after alizarin red treatment for 0, 3, 7, 10, and 14 h. The key pathways and key enzymes involved in alizarin red degradation were found to be though the analysis of KEGG, GO and COG. The GST, MnP and Laccase enzyme activities of L. gibbosa treated with alizarin red for 0–14 h were detected. LC-MS and GC-MS analyses of alizarin red decomposition products after 7 h and 14 h were performed. Results The glutathione metabolic pathway ko00480, and the key enzymes GST, MnP, Laccase and CYP450 were selected. Most of the genes encoding these enzymes were upregulated under alizarin red conditions. The GST activity increased 1.8 times from 117.55 U/mg prot at 0 h to 217.03 U/mg prot at 14 h. The MnP activity increased 2.9 times from 6.45 U/L to 18.55U/L. The Laccase activity increased 3.7 times from 7.22 U/L to 27.28 U/L. Analysis of the alizarin red decolourization rate showed that the decolourization rate at 14 h reached 20.21%. The main degradation intermediates were found to be 1,4-butene diacid, phthalic acid, 1,1-diphenylethylene, 9,10-dihydroanthracene, 1,2-naphthalene dicarboxylic acid, bisphenol, benzophenol-5,2-butene, acrylaldehyde, and 1-butylene, and the degradation process of AR was inferred. Overall, 1,4-butene diacid is the most important intermediate product produced by AR degradation. Conclusions The glutathione metabolic pathway was the key pathway for AR degradation. GST, MnP, Laccase and CYP450 were the key enzymes for AR degradation. 1,4-butene diacid is the most important intermediate product. This study explored the process of AR biodegradation at the molecular and biochemical levels and provided a theoretical basis for its application in practical production.

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