scholarly journals Carbon Black Nanoparticles Selectively Alter Follicle-Stimulating Hormone Expression in vitro and in vivo in Female Mice

2021 ◽  
Vol 15 ◽  
Author(s):  
Charlotte Avet ◽  
Emmanuel N. Paul ◽  
Ghislaine Garrel ◽  
Valérie Grange-Messent ◽  
David L’Hôte ◽  
...  
Keyword(s):  
Carbon Black ◽  
Follicle Stimulating Hormone ◽  
Primary Cultures ◽  
Pituitary Cells ◽  
Intratracheal Administration ◽  
Female Mice ◽  
Carbon Black Nanoparticles ◽  
Stimulating Hormone

Toxic effects of nanoparticles on female reproductive health have been documented but the underlying mechanisms still need to be clarified. Here, we investigated the effect of carbon black nanoparticles (CB NPs) on the pituitary gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH), which are key regulators of gonadal gametogenesis and steroidogenesis. To that purpose, we subjected adult female mice to a weekly non-surgical intratracheal administration of CB NPs at an occupationally relevant dose over 4 weeks. We also analyzed the effects of CB NPs in vitro, using both primary cultures of pituitary cells and the LβT2 gonadotrope cell line. We report here that exposure to CB NPs does not disrupt estrous cyclicity but increases both circulating FSH levels and pituitary FSH β-subunit gene (Fshb) expression in female mice without altering circulating LH levels. Similarly, treatment of anterior pituitary or gonadotrope LβT2 cells with increasing concentrations of CB NPs dose-dependently up-regulates FSH but not LH gene expression or release. Moreover, CB NPs enhance the stimulatory effect of GnRH on Fshb expression in LβT2 cells without interfering with LH regulation. We provide evidence that CB NPs are internalized by LβT2 cells and rapidly activate the cAMP/PKA pathway. We further show that pharmacological inhibition of PKA significantly attenuates the stimulatory effect of CB NPs on Fshb expression. Altogether, our study demonstrates that exposure to CB NPs alters FSH but not LH expression and may thus lead to gonadotropin imbalance.

PHYSIOLOGICAL ACTIONS OF HUMAN FOLLICLE-STIMULATING HORMONE AND ITS β-SUBUNIT IN REPTILES

1977 ◽  
Vol 74 (3) ◽  
pp. 441-447 ◽  
Author(s):  
PAUL LICHT ◽  
ANTONELLA BONA GALLO ◽  
ANNE STOCKELL HARTREE ◽  
RATNA C. SHOWNKEEN
Keyword(s):  
Biological Activity ◽  
Follicle Stimulating Hormone ◽  
Β Subunit ◽  
Binding Assays ◽  
Mammalian Tissues ◽  
Stimulation Of ◽  
Stimulating Hormone

SUMMARY The actions of human follicle-stimulating hormone (hFSH) and its β-subunit were examined in several assays in reptiles, including effects on lizard testicular activity (growth and androgen production) in vivo, and stimulation of androgen production by snake testes and competition for binding of 125I-labelled hFSH in lizards and snakes in vitro. Binding was also examined with mammalian tissues. The hFSH was highly steroidogenic in the snake and lizard; otherwise results were similar to those observed in mammals. In all cases, the potency of the β-subunit was only a few per cent of the intact hormone. The potency of hFSH in vivo compared with NIH-FSH ovine standards was several 100 times greater than in vitro. Results for stimulation of androgen production in vivo closely paralleled those for binding assays in both reptiles and mammals. In contrast to previous results for ovine FSH β-subunit, human FSH β-subunit has little if any FSH biological activity in reptiles.

Inhibition of release of a novel pituitary polypeptide, 7B2, follicle-stimulating hormone, and luteinizing hormone from rat anterior pituitary cells in vitro by human β-inhibin

1986 ◽  
Vol 64 (9) ◽  
pp. 1259-1262 ◽  
Author(s):  
John S. D. Chan ◽  
Jie-Ying Deng ◽  
Anoop K. Brar ◽  
Nabil G. Seidah ◽  
Michel Chrétien
Keyword(s):  
Luteinizing Hormone ◽  
Anterior Pituitary ◽  
Polyclonal Antibodies ◽  
Follicle Stimulating Hormone ◽  
Feedback Mechanism ◽  
Pituitary Cells ◽  
Anterior Pituitary Cells ◽  
Specific Radioimmunoassay ◽  
Stimulating Hormone

We have recently purified a novel pituitary polypeptide designated 7B2. By raising polyclonal antibodies to a synthetic 7B2 fragment in rabbits, we have developed a sensitive and specific radioimmunoassay for this novel polypeptide, and it has been used for the study of the release of immunoreactive 7B2 from rat anterior pituitary cells in vitro. In addition, immunocytochemical study shows that 7B2 is present in the gonadotropin cells of rat anterior pituitary. The aim of the present studies is to investigate the effect of human β-inhibin, testosterone, and combined testosterone plus human β-inhibin on the induced release of immunoreactive 7B2, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) in rat anterior pituitary cell culture in vitro. Our results show that both human β-inhibin and testosterone effectively suppress the stimulatory effect of luteinizing hormone-releasing hormone (LHRH) on immunoreactive 7B2, FSH, and LH release. The present data indicate that the regulation of secretion of 7B2 and pituitary gonadotropins may be under a similar type of feedback mechanism.

scholarly journals Inhibin B Is a More Potent Suppressor of Rat Follicle-Stimulating Hormone Release than Inhibin A in Vitro and in Vivo

Endocrinology ◽  
2009 ◽  
Vol 150 (10) ◽  
pp. 4784-4793 ◽  
Author(s):  
Yogeshwar Makanji ◽  
Peter D. Temple-Smith ◽  
Kelly L. Walton ◽  
Craig A. Harrison ◽  
David M. Robertson
Keyword(s):  
Follicle Stimulating Hormone ◽  
Inhibin B ◽  
Hormone Release ◽  
Inhibin A ◽  
Stimulating Hormone

EVALUATION OF THE BOVINE TESTICULAR RADIORECEPTOR ASSAY FOR PITUITARY FOLLICLE-STIMULATING HORMONE

1979 ◽  
Vol 82 (2) ◽  
pp. 253-262 ◽  
Author(s):  
M. R. SAIRAM
Keyword(s):  
Biological Activities ◽  
Follicle Stimulating Hormone ◽  
Radioreceptor Assay ◽  
In Vitro Assays ◽  
Receptor Interactions ◽  
Testicular Receptor ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Stimulating Hormone

SUMMARY A modified bovine testicular receptor was used to evaluate highly purified follicle-stimulating hormone (FSH) from a number of species. The particulate receptor obtained from adult bovine testes could be stored frozen or lyophilized for long periods without appreciable decrease in the binding of the ligand facility or in loss of specificity. The bovine testis receptor binds twice as much 125I-labelled ovine FSH as 125I-labelled human hormone. When FSH from different species was compared against NIH-FSH-S10, using various FSH ligands, the ovine hormone was clearly the most active, although many had comparable in-vivo biological potencies. The results suggest that there is probably some species specificity in the hormone–receptor interactions. As the ovine hormone is structurally closer to the bovine, from which the receptor was derived, it appears to have the highest activity in vitro. Marked differences in the biological activities of the different preparations between the human chorionic gonadotrophin-augmentation test and the in-vitro assays have been observed. In the in-vitro assays, all preparations, with the exception of the porcine hormone preparation, were less active and the ratio of bioassay/radioreceptor assay varied widely. In the radioreceptor assays, all FSH preparations except pregnant mare serum gonadotrophin (PMSG) showed parallel inhibition curves. The three different PMSG preparations examined gave inhibition lines that were parallel to each other.

Effects of crude and highly purified bovine inhibin (Mr 32 000 form) on gonadotrophin production by ovine pituitary cells in vitro: inhibin enhances gonadotrophin-releasing hormone-induced release of LH

1990 ◽  
Vol 127 (1) ◽  
pp. 149-159 ◽  
Author(s):  
S. Muttukrishna ◽  
P. G. Knight
Keyword(s):  
Primary Cultures ◽  
Suppressive Effect ◽  
Pituitary Cells ◽  
Dependent Manner ◽  
Α Subunit ◽  
Releasing Hormone ◽  
Lh Release ◽  
Gonadotrophin Releasing Hormone

ABSTRACT Primary cultures of ovine pituitary cells (from adult ewes) were used to investigate the actions of steroid-free bovine follicular fluid (bFF) and highly-purified Mr 32 000 bovine inhibin on basal and gonadotrophin-releasing hormone (GnRH)-induced release of FSH and LH. Residual cellular contents of each hormone were also determined allowing total gonadotrophin content/well to be calculated. As in rats, both crude and highly purified inhibin preparations promoted a dose (P < 0·001)- and time (P < 0·001)-dependent suppression of basal and GnRH-induced release of FSH as well as an inhibition of FSH synthesis, reflected by a fall in total FSH content/well. However, while neither inhibin preparation affected basal release of LH or total LH content/well, GnRH-induced LH release was significantly (P< 0·001) increased by the presence of either bFF (+ 75%) or highly-purified inhibin (+ 64%) in a dose- and time-dependent manner. This unexpected action of bFF on GnRH-induced LH release was abolished in the presence of 5 μl specific anti-inhibin serum, confirming that the response was indeed mediated by inhibin. Furthermore, neither oestradiol-17β (1 pmol/l–10 nmol/l) nor monomeric α-subunit of bovine inhibin (2·5–40 ng/ml) significantly affected basal or GnRH-induced release of LH. These in-vitro findings for the ewe lend support to a number of recent in-vivo observations and indicate that, in addition to its well-documented suppressive effect on the synthesis and secretion of FSH, inhibin may actually facilitate LH release in this species, in marked contrast to its action in the rat. Journal of Endocrinology (1990) 127, 149–159

Follicle-Stimulating Hormone Does Not Impact Male Bone Mass In Vivo or Human Male Osteoclasts In Vitro

2008 ◽  
Vol 82 (5) ◽  
pp. 383-391 ◽  
Author(s):  
Veronique Ritter ◽  
Barbara Thuering ◽  
Pierre Saint Mezard ◽  
Ngoc-Hong Luong-Nguyen ◽  
Yves Seltenmeyer ◽  
...  
Keyword(s):  
Bone Mass ◽  
Follicle Stimulating Hormone ◽  
Human Male ◽  
Stimulating Hormone

A PRIMING EFFECT OF LUTEINIZING HORMONE RELEASING FACTOR WITH RESPECT TO RELEASE OF FOLLICLE-STIMULATING HORMONE IN VITRO AND IN VIVO

1977 ◽  
Vol 75 (1) ◽  
pp. 155-159 ◽  
Author(s):  
A. J. M. C. PICKERING ◽  
G. FINK
Keyword(s):  
Protein Synthesis ◽  
Luteinizing Hormone ◽  
Priming Effect ◽  
Follicle Stimulating Hormone ◽  
Stimulating Hormone

Pituitary incubation studies were carried out which showed that in the rat luteinizing hormone releasing factor (LH-RF) can exert a priming effect on FSH secretion in vitro. It was found that, as for LH, the effect depends on protein synthesis. The priming effect of LH-RF with respect to FSH could also be demonstrated in vivo; however, the effect was less dramatic than for LH.

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