scholarly journals Comprehensive Molecular Characterizations of Chinese Patients With Different Subtypes of Lung Squamous Cell Carcinoma

2020 ◽  
Vol 10 ◽  
Author(s):  
Jie Qian ◽  
Rongrong Chen ◽  
Ruiying Zhao ◽  
Yuchen Han ◽  
Yongfeng Yu
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Lung Squamous Cell Carcinoma ◽  
Chinese Patients ◽  
Targeted Next Generation Sequencing ◽  
Keratinizing Squamous Cell Carcinoma ◽  
Molecular Prognosis ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing

BackgroundThis study aims to profile integrative genomic spectra of Chinese patients with different subtypes of lung squamous cell carcinoma (LUSC) and explore potential molecular prognosis factors.MethodsWe retrospectively identified 204 surgically resected LUSC patients in Shanghai Chest Hospital who underwent capture-based targeted next-generation sequencing (NGS) with a panel of 68 lung cancer‐related genes from September 2017 to January 2019. NGS was used to profile comprehensive molecular characterizations.ResultsOf 204 cases, 114 (55.9%) were keratinizing squamous cell carcinoma (KSCC), 77 (37.7%) were non-keratinizing squamous cell carcinoma (NKSCC), 13 (6.4%) were basaloid squamous cell carcinoma (BSCC), respectively. All subtypes presented similarly high proportions of mutations, including TP53, CDKN2A, and NOTCH1. A comparable prevalence of FGFR1 amplifications was identified between KSCC and NKSCC (11.4 versus 26.9%, p = 0.007). Compared with NKSCC, IGF1R amplifications were more frequent in BSCC (0 versus 15.4%, p = 0.019). We found cases with TP53 alterations had less EGFR alterations in KSCC (P = 0.013, OR = 0.158). Compared with TCGA cohorts, our Chinese cohorts exhibited statistic differences in both somatic mutations and signaling pathways. We found that STK 11 alterations and TOP2A alterations were significantly associated with higher risk of recurrence in patients with LUSC.ConclusionsSignificant differences exist among three subtypes of LUSC in molecular characterizations.

scholarly journals Mutations and expression of the NFE2L2/KEAP1/CUL3 pathway in Chinese patients with lung squamous cell carcinoma

2016 ◽  
Vol 8 (7) ◽  
pp. 1639-1644 ◽  
Author(s):  
Yongxing Zhang ◽  
Hong Fan ◽  
Shuo Fang ◽  
Lin Wang ◽  
Li Chen ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Lung Squamous Cell Carcinoma ◽  
Chinese Patients

scholarly journals An Integrated Inspection of the Somatic Mutations in a Lung Squamous Cell Carcinoma Using Next-Generation Sequencing

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e78823 ◽  
Author(s):  
Lucy F. Stead ◽  
Philip Egan ◽  
Aoife Devery ◽  
Caroline Conway ◽  
Catherine Daly ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Next Generation Sequencing ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Somatic Mutations ◽  
Lung Squamous Cell Carcinoma ◽  
Next Generation ◽  
Generation Sequencing

scholarly journals Episomal HPV16 responsible for aggressive and deadly metastatic anal squamous cell carcinoma evidenced in peripheral blood

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hélène Péré ◽  
Raphael Vernet ◽  
Simon Pernot ◽  
Juliette Pavie ◽  
Nicolas Robillard ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Tissue Samples ◽  
Anal Squamous Cell Carcinoma ◽  
Hpv Dna ◽  
Hpv Status ◽  
Episomal Hpv16 ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing

AbstractArchival tissue samples collected longitudinally from a patient who died from HPV16-induced high-grade anal intraepithelial squamous cell carcinoma with vertebral HPV16–positive metastasis were retrospectively analyzed by the Capture-HPV method (Capt-HPV) followed by Next-Generation Sequencing (NGS). Full length nucleotide sequences of the same HPV16 were identified from the initial and second anal biopsy samples, from plasma sample and from vertebral metastasis biopsy. Remarkably, HPV was episomal in each sample. The HPV genome sequence was closest to the HPV16 Qv18158E variant subtype (A1 lineage) exhibiting base substitutions and deletions in 7 and 2 HPV loci, respectively. In conclusion, the powerful Capt-HPV followed by NGS allows evidencing the detailed cartography of tumoral and circulating HPV DNA, giving rise to a unique and unexpected episomal virus molecular status in a context of aggressive carcinoma, underlying the importance of HPV status and its association with clinical features for further prospective studies.

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