Tumor DNA From Tumor In Situ Fluid Reveals Mutation Landscape of Minimal Residual Disease After Glioma Surgery and Risk of Early Recurrence

The recurrence of glioma is a difficult problem in clinical treatment. The molecular markers of primary tumors after resection cannot fully represent the characteristics of recurrent tumors. Here, abundant tumor DNA was detected in tumor in situ fluid (TISF). We report that TISF-derived tumor DNA (TISF-DNA) can detect genomic changes in recurrent tumors and facilitate recurrence risk analysis, providing valuable information for diagnosis and prognosis. The tumor DNA in TISF is more representative and sensitive than that in cerebrospinal fluid. It reveals the mutational landscape of minimal residual disease after glioma surgery and the risk of early recurrence, contributing to the clinical management and clinical research of glioma patients.

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Tumor In Situ Fluid Was Used to Characterize Glioma in Real-Time to Monitor Minimal Residual Disease and Tumor DNA Relapse

SSRN Electronic Journal ◽

10.2139/ssrn.3835749 ◽

2021 ◽

Author(s):

Jinliang Yu ◽

Zhiyuan Sheng ◽

Yushuai Gao ◽

Zhaoyue Yan ◽

Chaojie Bu ◽

...

Keyword(s):

Real Time ◽

Minimal Residual Disease ◽

Residual Disease ◽

Tumor Dna ◽

Minimal Residual

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P-24 Circulating tumor DNA as a marker of Minimal Residual Disease in squamous cell carcinoma of the head and neck: an agnostic approach

Oral Oncology ◽

10.1016/s1368-8375(21)00313-4 ◽

2021 ◽

Vol 118 ◽

pp. 11

Author(s):

N. Honoré ◽

R. Galot ◽

C. van Marcke ◽

R. Helaers ◽

A. Mendola ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Head And Neck ◽

Minimal Residual Disease ◽

Squamous Cell ◽

Residual Disease ◽

Circulating Tumor Dna ◽

Tumor Dna ◽

Minimal Residual

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Patient-Specific Circulating Tumor DNA Detection during Neoadjuvant Chemotherapy in Triple-Negative Breast Cancer

Clinical Chemistry ◽

10.1373/clinchem.2016.262337 ◽

2017 ◽

Vol 63 (3) ◽

pp. 691-699 ◽

Cited By ~ 72

Author(s):

Francesca Riva ◽

Francois-Clement Bidard ◽

Alexandre Houy ◽

Adrien Saliou ◽

Jordan Madic ◽

...

Keyword(s):

Breast Cancer ◽

Neoadjuvant Chemotherapy ◽

Minimal Residual Disease ◽

Triple Negative Breast Cancer ◽

Triple Negative ◽

Residual Disease ◽

Circulating Tumor Dna ◽

Tp53 Mutations ◽

Tumor Dna ◽

Minimal Residual

Abstract BACKGROUND In nonmetastatic triple-negative breast cancer (TNBC) patients, we investigated whether circulating tumor DNA (ctDNA) detection can reflect the tumor response to neoadjuvant chemotherapy (NCT) and detect minimal residual disease after surgery. METHODS Ten milliliters of plasma were collected at 4 time points: before NCT; after 1 cycle; before surgery; after surgery. Customized droplet digital PCR (ddPCR) assays were used to track tumor protein p53 (TP53) mutations previously characterized in tumor tissue by massively parallel sequencing (MPS). RESULTS Forty-six patients with nonmetastatic TNBC were enrolled. TP53 mutations were identified in 40 of them. Customized ddPCR probes were validated for 38 patients, with excellent correlation with MPS (r = 0.99), specificity (≥2 droplets/assay), and sensitivity (at least 0.1%). At baseline, ctDNA was detected in 27/36 patients (75%). Its detection was associated with mitotic index (P = 0.003), tumor grade (P = 0.003), and stage (P = 0.03). During treatment, we observed a drop of ctDNA levels in all patients but 1. No patient had detectable ctDNA after surgery. The patient with rising ctDNA levels experienced tumor progression during NCT. Pathological complete response (16/38 patients) was not correlated with ctDNA detection at any time point. ctDNA positivity after 1 cycle of NCT was correlated with shorter disease-free (P < 0.001) and overall (P = 0.006) survival. CONCLUSIONS Customized ctDNA detection by ddPCR achieved a 75% detection rate at baseline. During NCT, ctDNA levels decreased quickly and minimal residual disease was not detected after surgery. However, a slow decrease of ctDNA level during NCT was strongly associated with shorter survival.

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