scholarly journals Identification of Intracellular β-Barrel Residues Involved in Ion Selectivity in the Mechanosensitive Channel of Thermoanaerobacter tengcongensis

2017 ◽  
Vol 8 ◽  
Author(s):  
Yingcai Song ◽  
Bing Zhang ◽  
Fei Guo ◽  
Maojun Yang ◽  
Yang Li ◽  
...  
Keyword(s):  
Ion Selectivity ◽  
Mechanosensitive Channel ◽  
Thermoanaerobacter Tengcongensis

Thermal Stability of Thermoanaerobacter tengcongensis Ribosome Recycling Factor

2014 ◽  
Vol 21 (3) ◽  
pp. 285-291 ◽  
Author(s):  
Yi Shi ◽  
Dongyan Zheng ◽  
Jingyi Xie ◽  
Qijun Zhang ◽  
Hongjie Zhang
Keyword(s):  
Thermal Stability ◽  
Ribosome Recycling Factor ◽  
Thermoanaerobacter Tengcongensis ◽  
Thermal Stability Of

A New Polyethylene Corrosion Protecting Coating with Ion Selectivity

2011 ◽  
Vol 314-316 ◽  
pp. 273-278
Author(s):  
Yu Hua Dong ◽  
Ke Ren ◽  
Qiong Zhou
Keyword(s):  
Ion Selectivity ◽  
Nano Particles ◽  
Ammonium Bromide ◽  
Linear Low Density Polyethylene ◽  
X Ray Diffraction ◽  
Sulfosalicylic Acid ◽  
X Ray ◽  
Chemically Modified ◽  
Before And After ◽  
Industrial Standards

Linear low density polyethylene (LLDPE) was chemically modified with grafting maleic anhydride (MAH) monomer on its backbone by melting blending. Nano-particles SiO2 was modified by cationic surfactant hexadecyl trimethyl ammonium bromide (CTAB) and anionic surfactant sulfosalicylic acid (SSA) and added to PE coating respectively. Measurement of membrane potential showed that the coating containing modified SiO2 nano-particles had characteristic of ion selectivity. The properties of the different coatings were investigated according to relative industrial standards. Experimental results indicated that PE coating with ion selectivity had better performances, such as adhesion strength, cathodic disbonding and anti-corrosion, than those of coating without ion selectivity. Crystal structure of the coatings before and after alkali corrosion was characterized by Fourier transform infrared spectra (FTIR) and X-ray diffraction (XRD). Structure of the coating without ion selectivity was damaged by NaOH alkali solution, causing mechanical properties being decreased. And the structure of the ion selective coatings was not affected.

Usefulness of collaboration between mathematical models and cell engineering for elucidating complex disease mechanisms and discover effective drugs

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
H Kohjitani ◽  
A Kashiwa ◽  
T Makiyama ◽  
F Toyoda ◽  
Y Yamamoto ◽  
...  
Keyword(s):  
Mathematical Model ◽  
In Silico ◽  
Complex Disease ◽  
Ion Selectivity ◽  
Public Institution ◽  
Cell Engineering ◽  
Funding Source ◽  
Patch Clamp Technique ◽  
In Silico Model

Abstract Background A missense mutation, CACNA1C-E1115K, located in the cardiac L-type calcium channel (LTCC), was recently reported to be associated with diverse arrhythmias. Several studies reported in-vivo and in-vitro modeling of this mutation, but actual mechanism and target drug of this disease has not been clarified due to its complex ion-mechanisms. Objective To reveal the mechanism of this diverse arrhythmogenic phenotype using combination of in-vitro and in-silico model. Methods and results Cell-Engineering Phase: We generated human induced pluripotent stem cell (hiPSC) from a patient carrying heterozygous CACNA1C-E1115K and differentiated into cardiomyocytes. Spontaneous APs were recorded from spontaneously beating single cardiomyocytes by using the perforated patch-clamp technique. Mathematical-Modeling Phase: We newly developed ICaL-mutation mathematical model, fitted into experimental data, including its impaired ion selectivity. Furthermore, we installed this mathematical model into hiPSC-CM simulation model. Collaboration Phase: Mutant in-silico model showed APD prolongation and frequent early afterdepolarization (EAD), which are same as in-vitro model. In-silico model revealed this EAD was mostly related to robust late-mode of sodium current occurred by Na+ overload and suggested that mexiletine is capable of reducing arrhythmia. Afterward, we applicated mexiletine onto hiPSC-CMs mutant model and found mexiletine suppress EADs. Conclusions Precise in-silico disease model can elucidate complicated ion currents and contribute predicting result of drug-testing. Funding Acknowledgement Type of funding source: Public Institution(s). Main funding source(s): Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists

scholarly journals Alteration of the pH-dependent ion selectivity of the colicin E1 channel by site-directed mutagenesis.

1990 ◽  
Vol 265 (12) ◽  
pp. 6984-6991
Author(s):  
K S Jakes ◽  
C K Abrams ◽  
A Finkelstein ◽  
S L Slatin
Keyword(s):  
Ion Selectivity ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Colicin E1 ◽  
Ph Dependent
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