scholarly journals Action of Actomyosin Contraction With Shh Modulation Drive Epithelial Folding in the Circumvallate Papilla

2020 ◽  
Vol 11 ◽  
Author(s):  
Sushan Zhang ◽  
Jong-Min Lee ◽  
Adpaikar Anish Ashok ◽  
Han-Sung Jung
Keyword(s):  
Circumvallate Papilla ◽  
Actomyosin Contraction

Lectin Histochemistry of Taste Buds of the Rat Tongue Circumvallate Papilla under Normal Conditions and in Glutamate-Induced Obesity

2018 ◽  
Vol 3 (4) ◽  
pp. 194-198
Author(s):  
Ya. I. Nych ◽  
◽  
A. M. Yaschenko ◽  
A. D. Lutsyk
Keyword(s):  
Lectin Histochemistry ◽  
Taste Buds ◽  
Circumvallate Papilla ◽  
Rat Tongue

Grhl3 modulates epithelial structure formation of the circumvallate papilla during mouse development

2016 ◽  
Vol 147 (1) ◽  
pp. 5-16 ◽  
Author(s):  
Nirpesh Adhikari ◽  
Sanjiv Neupane ◽  
Gi-Jeong Gwon ◽  
Ji-Youn Kim ◽  
Chang-Hyeon An ◽  
...  
Keyword(s):  
Structure Formation ◽  
Mouse Development ◽  
Circumvallate Papilla ◽  
Epithelial Structure

scholarly journals Collagen I triggers directional migration, invasion and matrix remodeling of stroma cells in a 3D spheroid model of endometriosis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Anna Stejskalová ◽  
Victoria Fincke ◽  
Melissa Nowak ◽  
Yvonne Schmidt ◽  
Katrin Borrmann ◽  
...  
Keyword(s):  
Single Cells ◽  
Fold Increase ◽  
Experimental Models ◽  
Collagen I ◽  
Matrix Remodeling ◽  
Clinical Tool ◽  
Directional Migration ◽  
Endometrial Cells ◽  
Small Molecule Drugs ◽  
Actomyosin Contraction

AbstractEndometriosis is a painful gynecological condition characterized by ectopic growth of endometrial cells. Little is known about its pathogenesis, which is partially due to a lack of suitable experimental models. Here, we use endometrial stromal (St-T1b), primary endometriotic stromal, epithelial endometriotic (12Z) and co-culture (1:1 St-T1b:12Z) spheroids to mimic the architecture of endometrium, and either collagen I or Matrigel to model ectopic locations. Stromal spheroids, but not single cells, assumed coordinated directional migration followed by matrix remodeling of collagen I on day 5 or 7, resembling ectopic lesions. While generally a higher area fold increase of spheroids occurred on collagen I compared to Matrigel, directional migration was not observed in co-culture or in 12Z cells. The fold increase in area on collagen I was significantly reduced by MMP inhibition in stromal but not 12Z cells. Inhibiting ROCK signalling responsible for actomyosin contraction increased the fold increase of area and metabolic activity compared to untreated controls on Matrigel. The number of protrusions emanating from 12Z spheroids on Matrigel was decreased by microRNA miR-200b and increased by miR-145. This study demonstrates that spheroid assay is a promising pre-clinical tool that can be used to evaluate small molecule drugs and microRNA-based therapeutics for endometriosis.

scholarly journals Species generalization and differences in Hedgehog pathway regulation of fungiform and circumvallate papilla taste function and somatosensation demonstrated with sonidegib

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
A. Kumari ◽  
Y. Yokota ◽  
L. Li ◽  
R. M. Bradley ◽  
C. M. Mistretta
Keyword(s):  
Hedgehog Pathway ◽  
Circumvallate Papilla ◽  
Pathway Regulation

scholarly journals Actomyosin Contraction Induces In-Bulk Motility of Cells and Droplets

2020 ◽  
Vol 119 (5) ◽  
pp. 1025-1032
Author(s):  
Thomas Le Goff ◽  
Benno Liebchen ◽  
Davide Marenduzzo
Keyword(s):  
Actomyosin Contraction

scholarly journals Cell migration: Fibroblasts find a new way to get ahead

2012 ◽  
Vol 197 (3) ◽  
pp. 347-349 ◽  
Author(s):  
Michael Sixt
Keyword(s):  
Cell Migration ◽  
Focal Adhesions ◽  
Leading Edge ◽  
Two Dimensional ◽  
Tissue Explants ◽  
Cell Biol ◽  
Membrane Blebs ◽  
3D Environments ◽  
Actomyosin Contraction ◽  
Phosphatidylinositol 3

Fibroblasts migrate on two-dimensional (2D) surfaces by forming lamellipodia—actin-rich extensions at the leading edge of the cell that have been well characterized. In this issue, Petrie et al. (2012. J. Cell Biol. http://dx.doi.org/10.1083/jcb.201201124) show that in some 3D environments, including tissue explants, fibroblasts project different structures, termed lobopodia, at the leading edge. Lobopodia still assemble focal adhesions; however, similar to membrane blebs, they are driven by actomyosin contraction and do not accumulate active Rac, Cdc42, and phosphatidylinositol 3-kinases.

scholarly journals DMPK is a New Candidate Mediator of Tumor Suppressor p53-Dependent Cell Death

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3175
Author(s):  
Katsuhiko Itoh ◽  
Takahiro Ebata ◽  
Hiroaki Hirata ◽  
Takeru Torii ◽  
Wataru Sugimoto ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cell Death ◽  
Tumor Suppressor ◽  
Dependent Manner ◽  
Tumor Suppressor P53 ◽  
P53 Family ◽  
Gene Encoding ◽  
Myotonic Dystrophy Protein Kinase ◽  
Induced Apoptosis ◽  
Actomyosin Contraction

Tumor suppressor p53 plays an integral role in DNA-damage induced apoptosis, a biological process that protects against tumor progression. Cell shape dramatically changes when cells undergo apoptosis, which is associated with actomyosin contraction; however, it remains entirely elusive how p53 regulates actomyosin contraction in response to DNA-damaging agents. To identify a novel p53 regulating gene encoding the modulator of myosin, we conducted DNA microarray analysis. We found that, in response to DNA-damaging agent doxorubicin, expression of myotonic dystrophy protein kinase (DMPK), which is known to upregulate actomyosin contraction, was increased in a p53-dependent manner. The promoter region of DMPK gene contained potential p53-binding sequences and its promoter activity was increased by overexpression of the p53 family protein p73, but, unexpectedly, not of p53. Furthermore, we found that doxorubicin treatment induced p73 expression, which was significantly attenuated by downregulation of p53. These data suggest that p53 induces expression of DMPK through upregulating p73 expression. Overexpression of DMPK promotes contraction of the actomyosin cortex, which leads to formation of membrane blebs, loss of cell adhesion, and concomitant caspase activation. Taken together, our results suggest the existence of p53-p73-DMPK axis which mediates DNA-damage induced actomyosin contraction at the cortex and concomitant cell death.

scholarly journals FGF10 Is Required for Circumvallate Papilla Morphogenesis by Maintaining Lgr5 Activity

2018 ◽  
Vol 9 ◽  
Author(s):  
Sushan Zhang ◽  
Hyuk Su Choi ◽  
Han-Sung Jung ◽  
Jong-Min Lee
Keyword(s):  
Circumvallate Papilla

scholarly journals Cross-linker–mediated regulation of actin network organization controls tissue morphogenesis

2019 ◽  
Vol 218 (8) ◽  
pp. 2743-2761 ◽  
Author(s):  
Daniel Krueger ◽  
Theresa Quinkler ◽  
Simon Arnold Mortensen ◽  
Carsten Sachse ◽  
Stefano De Renzis
Keyword(s):  
Myosin Ii ◽  
Temporal Organization ◽  
Tissue Morphogenesis ◽  
Animal Development ◽  
Spatio Temporal ◽  
Development In Vitro ◽  
Short Time ◽  
Actomyosin Contraction

Contraction of cortical actomyosin networks driven by myosin activation controls cell shape changes and tissue morphogenesis during animal development. In vitro studies suggest that contractility also depends on the geometrical organization of actin filaments. Here we analyze the function of actomyosin network topology in vivo using optogenetic stimulation of myosin-II in Drosophila embryos. We show that early during cellularization, hexagonally arrayed actomyosin fibers are resilient to myosin-II activation. Actomyosin fibers then acquire a ring-like conformation and become contractile and sensitive to myosin-II. This transition is controlled by Bottleneck, a Drosophila unique protein expressed for only a short time during early cellularization, which we show regulates actin bundling. In addition, it requires two opposing actin cross-linkers, Filamin and Fimbrin. Filamin acts synergistically with Bottleneck to facilitate hexagonal patterning, while Fimbrin controls remodeling of the hexagonal network into contractile rings. Thus, actin cross-linking regulates the spatio-temporal organization of actomyosin contraction in vivo, which is critical for tissue morphogenesis.

scholarly journals Transganglionic transport of HRP from the circumvallate papilla of the rat

1985 ◽  
Vol 361 (1-2) ◽  
pp. 154-161 ◽  
Author(s):  
Robert M. Bradley ◽  
Charlotte M. Mistretta ◽  
Carolyn A. Bates ◽  
Herbert P. Killackey
Keyword(s):  
Circumvallate Papilla
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