scholarly journals It’s All About Timing: Early Feeding Promotes Intestinal Maturation by Shifting the Ratios of Specialized Epithelial Cells in Chicks

2020 ◽  
Vol 11 ◽  
Author(s):  
Naama Reicher ◽  
Tal Melkman-Zehavi ◽  
Jonathan Dayan ◽  
Zehava Uni
Keyword(s):  
Epithelial Cells ◽  
Goblet Cells ◽  
Histochemical Staining ◽  
Total Cell ◽  
Early Feeding ◽  
Differentiated Cells ◽  
First Feeding ◽  
Intestinal Maturation ◽  
Hatch Period

The small intestine (SI) of chicks (Gallus gallus) matures rapidly during the initial post-hatch period and acquires digestive, absorptive, and secretive capabilities. The effects of the timing of first feeding on the quantities and distribution of specialized epithelial cells, which generate and maintain SI morphology and functionality, have not yet been examined. In this study, we identified specialized SI epithelial cell sub-types, including stem, progenitor, proliferating, and differentiated cells within crypts and villi of chicks during the first 10 days post-hatch, by in situ hybridization (ISH), immunofluorescence (IF), and histochemical staining. We then examined their quantities and ratios between day of hatch and d10 in chicks that were fed upon hatch [early feeding (EF)], compared to chicks that were fed 24 h post-hatch [delayed feeding (DF)]. Results showed that EF increased total cell quantities in the crypts and villi at days 1, 3, 7, and 10, compared to DF (p < 0.0001). At d3, EF, in comparison to DF, decreased crypt stem cell proportions (p < 0.0001), increased crypt proliferating (p < 0.01) and differentiated (p < 0.05) cell proportions, and increased villus enterocyte proportions (p < 0.01). By d10, EF increased both the quantities and proportions of villus enterocytes and goblet cells, compared to DF. We conclude that feeding upon hatch, compared to 24 h-delayed feeding, enhanced SI maturation and functionality by increasing the quantities and proportions of proliferating and differentiated cells, thus expanding the digestive, absorptive, and secretive cell populations throughout the initial post-hatch period.

Influence of a lymphocyte-conditioned medium on the expression of smooth-muscle α-aktin in lens epithelial cells in situ

1999 ◽  
Vol 96 (3) ◽  
pp. 174-181
Author(s):  
Kerstin Wunderlich ◽  
Marcus Knorr ◽  
H. Northoff ◽  
Hans-J. Thiel
Keyword(s):  
Smooth Muscle ◽  
Epithelial Cells ◽  
Conditioned Medium ◽  
Lens Epithelial Cells

Ulex europaeus agglutinin 1 lectin histochemical staining of dog kidney and bile duct epithelial cells

1988 ◽  
Vol 45 (2) ◽  
pp. 272-273 ◽  
Author(s):  
P.J. BROWN ◽  
T.J. STEPHENSON
Keyword(s):  
Bile Duct ◽  
Epithelial Cells ◽  
Histochemical Staining ◽  
Ulex Europaeus ◽  
Dog Kidney

scholarly journals Abnormal mucus in cap polyposis

Gut ◽  
1998 ◽  
Vol 42 (1) ◽  
pp. 135-138 ◽  
Author(s):  
M P Buisine ◽  
J F Colombel ◽  
M Lecomte-Houcke ◽  
P Gower ◽  
J P Aubert ◽  
...  
Keyword(s):  
In Situ Hybridisation ◽  
Clinical Manifestations ◽  
Goblet Cells ◽  
Biopsy Specimens ◽  
Mucin Genes ◽  
Cap Polyposis ◽  
Abnormal Glycosylation ◽  
Histochemical Examination ◽  
Mrna In Situ Hybridisation

Background—Cap polyposis is a rare disease characterised by mucoid and bloody diarrhoea, with polyps covered by a cap of mucoid and fibrinopurulent exudate. The pathogenesis is not known.Aims—To pour some light on cap polyposis pathogenesis, by examining the mucus of patients and analysing the expression of five mucin genes, MUC2, MUC3,MUC4, MUC5AC, and MUC5B.Patient and methods—The study was performed on biopsy specimens taken from a patient with recurrent cap polyposis. Histochemical examination, electron microscopy, and mRNA in situ hybridisation were used.Results—The mucus of cap polyposis differed in three respects from that of normal adult colon: abnormal ultrastructure of the mucus in the goblet cells, predominance of non-sulphated mucins, abnormal expression of the MUC4, MUC3, andMUC5AC genes.Conclusions—Most of these abnormalities have been reported for other pathological situations, suggesting that the abnormalities observed in the mucus of this patient with cap polyposis are probably secondary phenomena rather than primary. However, the mucin abnormalities detected, which reflect deregulation of the expression of three apomucin genes, abnormal glycosylation, and abnormalities of the secretion process, are also probably involved in the clinical manifestations of cap polyposis.

Recombinant TIMP-1 and -2 enhance the proliferation of rabbit corneal epithelial cells in vitro and the spreading of rabbit corneal epithelium in situ

1998 ◽  
Vol 17 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Shizuya Saika ◽  
Yoshiji Kawashima ◽  
Yuka Okada ◽  
Sai-Ichi Tanaka ◽  
Osamu Yamanaka ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Corneal Epithelium ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial

Scanning electron microscopy of the operculum of Garra lamta (Hamilton) (Cyprinidae:Cypriniformes), an Indian hill stream fish

2010 ◽  
Vol 58 (3) ◽  
pp. 182 ◽  
Author(s):  
Swati Mittal ◽  
Usha Kumari ◽  
Pinky Tripathi ◽  
Ajay Kumar Mittal
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Epithelial Cells ◽  
Goblet Cells ◽  
Stream Fish ◽  
Male And Female ◽  
Fish Breeding ◽  
Inner Surface ◽  
Garra Lamta ◽  
Scanning Electron

The surface architecture of the epidermis on the outer surface of the operculum (OE) and the epithelium on the inner surface of the operculum (EISO) of Garra lamta was examined by scanning electron microscopy. The surface appeared smooth on the OE and wavy on the EISO. A wavy epithelium is considered to facilitate an increase in its stretchability, during the expansion of the branchial chamber. The OE and the EISO were covered by a mosaic pavement of epithelial cells with characteristic patterns of microridges and microbridges. Interspersed between the epithelial cells were mucous goblet cell pores, which were not significantly different in number in the OE and the EISO. Nevertheless, their surface area in the EISO was significantly higher than in the OE. This could be an adaptation to secrete higher amounts of mucus on the EISO for keeping the branchial chamber lining clean, avoiding clogging, the increased slipperiness reducing friction from water flow and increased efficiency in protecting against microbial attachments. Rounded bulges on the OE and the EISO were associated with mucous goblet cells. The absence of the taste buds in the EISO, in contrast to the OE, suggests that their function in the branchial chamber may not be of much significance in this fish. Breeding tubercles on the OE are believed to facilitate better contact between the male and female during breeding.

The Experimental Production of Goblet Cells and Inclusion Bodies

1947 ◽  
Vol s3-88 (3) ◽  
pp. 345-352
Author(s):  
E. MEIROWSKY ◽  
G. BEHR ◽  
S. KEYS
Keyword(s):  
Epithelial Cells ◽  
Inclusion Bodies ◽  
Goblet Cells ◽  
Experimental Production ◽  
Response Mechanism ◽  
Cytoplasmic Inclusions

1. Goblet cells have been produced experimentally in rabbits' and guineapigs' corneae by ten methods. 2. Goblet cells can develop from ordinary epithelial cells. 3. The secretion antecedents originate in the nucleus. 4. Intranuclear and cytoplasmic inclusions can be produced experimentally and form the response mechanism of the cell towards animate and inanimate stimuli.

Fine structure and development of schizonts, merozoites and macrogamonts of Eimeria acervulina in the goblet cells of the duodenal epithelium of experimentally infected birds

Parasitology ◽  
1975 ◽  
Vol 70 (2) ◽  
pp. 223-229 ◽  
Author(s):  
E. Michael
Keyword(s):  
Fine Structure ◽  
Epithelial Cells ◽  
Golgi Apparatus ◽  
Host Cell ◽  
Goblet Cells ◽  
Cellular Level ◽  
Site Specificity ◽  
Eimeria Acervulina ◽  
Duodenal Epithelium

The fine structure of trophozoites, schizonts, merozoites and macrogamonts of Eimeria acervulina found in goblet cells of the duodenal epithelium of chicks is described and compared with the corresponding stages formed in other epithelial cells. Complete schizogony, with the formation of mature merozoites, occurred freely in goblet cells. Developing macrogamonts (but no microgamonts) were rarely found in goblet cells. The stages observed were confined to the cytoplasm of the host cell above the Golgi apparatus and were usually seen between the mucous granules. The stages seen appeared normal, and contained similar structures to corresponding stages developing in other cells. The finding of developing stages of E. acervulina in goblet cells provides further evidence that site specificity of Eimeria at the cellular level is not as strict as previously thought.

scholarly journals Expression of the third member of the serum amyloid A gene family in mouse adipocytes.

1989 ◽  
Vol 169 (5) ◽  
pp. 1841-1846 ◽  
Author(s):  
E P Benditt ◽  
R L Meek
Keyword(s):  
Serum Amyloid A ◽  
Cell System ◽  
Serum Amyloid ◽  
Amyloid A ◽  
Differentiated Cells ◽  
Homologous Genes ◽  
Extrahepatic Tissues ◽  
Mouse Adipocytes ◽  
Related Proteins

Three homologous genes that code for three related proteins comprise the serum amyloid A (SAA) family in the mouse. Endotoxin induces equally vigorous expression of mRNAs for the three SAA genes in liver. In extrahepatic tissues SAA1 and/or SAA2 mRNAs have been found only in kidney and intestine, however, SAA3 is expressed in all extrahepatic tissues thus far examined. This observation raised the question: is SAA3 mRNA expressed by a single cell system dispersed throughout all tissues, or by differentiated cells of each tissue? This question was explored in various tissues by in situ hybridization with a single-stranded cRNA probe specific for SAA3 mRNA. We found expression in the liver of SAA3 mRNA by other cells as well as by hepatocytes. A common feature among extrahepatic tissues was SAA3 mRNA expression in adipocytes. SAA3 mRNA was also found in two nonadipose cells, Leydig cells of the testis, and some of the cells located in parafollicular zones of the spleen.

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