scholarly journals Histology and RNA Sequencing Provide Insights Into Fusarium Head Blight Resistance in AAC Tenacious

2021 ◽  
Vol 11 ◽  
Author(s):  
Kirby T. Nilsen ◽  
Sean Walkowiak ◽  
Santosh Kumar ◽  
Oscar I. Molina ◽  
Harpinder S. Randhawa ◽  
...  

Fusarium head blight (FHB) is a serious fungal disease affecting wheat and other cereals worldwide. This fungus causes severe yield and quality losses from a reduction in grain quality and contamination of grain with mycotoxins. Intensive breeding efforts led to the release of AAC Tenacious, which was the first spring wheat cultivar registered in Canada with a resistant (R) rating to FHB. To elucidate the physiological mechanisms of resistance, we performed histological and transcriptomic analyses of AAC Tenacious and a susceptible control Roblin after inoculation with Fusarium graminearum (Fg). The spikelet and rachis of infected wheat spikes were hand sectioned and monitored by confocal and fluorescent microscopy. Visible hyphae were observed within the inoculated spikelets for AAC Tenacious; however, the infection was largely restricted to the point of inoculation (POI), whereas the adjacent florets in Roblin were heavily infected. Significant cell wall thickening within the rachis node below the POI was evident in AAC Tenacious compared to Roblin in response to Fg inoculation. Rachis node and rachilla tissues from the POI and the rachis node below the POI were collected at 5 days post inoculation for RNAseq. Significant changes in gene expression were detected in both cultivars in response to infection. The rachis node below the POI in AAC Tenacious had fewer differentially expressed genes (DEGs) when compared to the uninoculated control, likely due to its increased disease resistance. Analysis of DEGs in Roblin and AAC Tenacious revealed the activation of genes and pathways in response to infection, including those putatively involved in cell wall modification and defense response.

Plant Science ◽  
2018 ◽  
Vol 274 ◽  
pp. 121-128 ◽  
Author(s):  
Angelica Giancaspro ◽  
Vincenzo Lionetti ◽  
Stefania L. Giove ◽  
Daniela Zito ◽  
Eleonora Fabri ◽  
...  

Author(s):  
Hussein M. Khaeim ◽  
Anthony Clark ◽  
Tom Pearson ◽  
Dr. David Van Sanford

Head scab is historically a devastating disease affecting not just all classes of wheat but also barley and other small grains around the world. Fusarium head blight (FHB), or head scab, is caused most often by Fusarium graminearum (Schwabe), (sexual stage – Gibberella zeae) although several Fusarium spp. can cause the disease. This study was conducted to determine the effect of mass selection for FHB resistance using an image-based optical sorter. lines were derived from the C0 and C2 of two populations to compare genetic variation within populations with and without sorter selection. Our overall hypothesis is that sorting grain results in improved Fusarium head blight resistance. Both of the used wheat derived line populations have genetic variation, and population 1 has more than population 17. They are significantly different from each other for fusarium damged kernel (FDK), deoxynivalenol (DON), and other FHB traits. Although both populations are suitable to be grown for bulks, population 1 seems better since it has more genetic variation as well as lower FDK and DON, and earlier heading date. Lines within each population were significantly different and some lines in each population had significantly lower FDK and DON after selection using an optical sorter. Some lines had significant reduction in both FDK and DON, and some others had either FDK or DON reduction. Lines of population 1 that had significant reduction, were more numerous than in population 17, and FDK and DON reduction were greater.


2007 ◽  
Vol 1 (1) ◽  
pp. 72-74
Author(s):  
Guo-Liang Jiang ◽  
ZhaoSu Wu ◽  
ZhaoXia Chen ◽  
JiMing Wu ◽  
QiMei Xia ◽  
...  

2018 ◽  
Vol 44 (4) ◽  
pp. 473 ◽  
Author(s):  
Zhan-Wang ZHU ◽  
Deng-An XU ◽  
Shun-He CHENG ◽  
Chun-Bao GAO ◽  
Xian-Chun XIA ◽  
...  

Author(s):  
David F. Garvin ◽  
Linda Dykes

AbstractWheat (Triticum aestivum L.) breeding involves improvement of a wide range of traits. However, selection for these traits is only acceptable if the end use quality of the wheat is not compromised. In hard red spring wheat, the predominant end use of flour is bread. In this study, milling and baking quality characteristics were compared in the hard red spring wheat ‘Apogee’ and a near-isogenic line of Apogee (‘A30’) that contains a spontaneous segmental deletion of the long arm of chromosome arm 3DL that is associated with enhanced resistance to Fusarium head blight caused by the fungal pathogen Fusarium graminearum (Schwabe). Apogee and A30 were grown together in replicated greenhouse experiments, and the resultant grain was used to compare a diverse spectrum of grain characteristics and milling and baking properties of the grain in the two wheat genotypes. The major difference detected was a significant increase in protein content in A30, which had nearly 21% more flour protein than Apogee. This difference did not affect any of the flour properties or baking characteristics evaluated, suggesting that the increased protein concentrations in A30 are not associated with the principal seed storage properties associated with baking quality. These results indicate that despite the size of the deletion in A30, no key genes associated with end use quality are located on that chromosome segment. The deletion may therefore find use in efforts to enhance Fusarium head blight in hard red spring wheat.


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