scholarly journals The Peptide PbrPSK2 From Phytosulfokine Family Induces Reactive Oxygen Species (ROS) Production to Regulate Pear Pollen Tube Growth

2020 ◽  
Vol 11 ◽  
Author(s):  
Xiaobing Kou ◽  
Qian Liu ◽  
Yangyang Sun ◽  
Peng Wang ◽  
Shaoling Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Tube ◽  
Gene Family ◽  
Pollen Tube Growth ◽  
Prunus Persica ◽  
Reactive Oxygen ◽  
Tube Growth ◽  
Evolutionary Analysis ◽  
Dependent Manner ◽  
Oxygen Species

Phytosulfokines (PSKs) are plant peptide growth factors that participate in multiple biological processes, including cell elongation and immune signaling. However, little is known about PSKs in Rosaceae species. Here, we identified 10 PSK genes in pear (Pyrus bretschneideri), 11 in apple (Malus × domestica), four in peach (Prunus persica), six in strawberry (Fragaria vesca), and five in Chinese plum (Prunus mume). In addition, we undertook comparative analysis of the PSK gene family in pear and the four other species. Evolutionary analysis indicated that whole genome duplication events (WGD) may have contributed to the expansion of the PSK gene family in Rosaceae. Transcriptomes, reverse transcription-PCR and quantitative real-time-PCR analyses were undertaken to demonstrate that PbrPSK2 is highly expressed in pear pollen. In addition, by adding purified E. coli-expressed PbrPSK2 to pollen and using an antisense oligonucleotide approach, we showed that PbrPSK2 can promote pear pollen tube elongation in a dose-dependent manner. Furthermore, PbrPSK2 was found to mediate the production of reactive oxygen species to regulate pear pollen tube growth.

scholarly journals PbrRALF2-elicited reactive oxygen species signaling is mediated by the PbrCrRLK1L13-PbrMPK18 module in pear pollen tubes

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Xiaobing Kou ◽  
Jiangmei Sun ◽  
Peng Wang ◽  
Danqi Wang ◽  
Peng Cao ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Reactive Oxygen ◽  
Pollen Tubes ◽  
Tube Growth ◽  
General Mechanism ◽  
Dependent Manner ◽  
Ros Production ◽  
Oxygen Species

AbstractRapid alkalinization factors (RALFs) are cysteine-rich peptides that play important roles in a variety of biological processes, such as cell elongation and immune signaling. Recent studies in Arabidopsis have shown that RALFs regulate pollen tube growth via plasma membrane receptor-like kinases (RLKs). However, the downstream signal transduction mechanisms of RLKs in pollen tubes are unknown. Here, we identified PbrRALF2, a pear (Pyrus bretschneideri) pollen RALF peptide that inhibits pollen tube growth. We found that PbrRALF2 interacts with a malectin-like domain-containing RLK, PbrCrRLK1L13. The relative affinity between PbrRALF2 and PbrCrRLK1L13 was at the submicromolar level, which is consistent with the values of ligand–receptor kinase pairs and the physiological concentration for PbrRALF2-mediated inhibition of pollen tube growth. After binding to its extracellular domain, PbrRALF2 activated the phosphorylation of PbrCrRLK1L13 in a dose-dependent manner. We further showed that the MAP kinase PbrMPK18 is a downstream target of PbrCrRLK1L13 that mediates PbrRALF2-elicited reactive oxygen species (ROS) production. The excessive accumulation of ROS inhibits pollen tube growth. We show that MPK acts as a mediator for CrRLK1L to stimulate ROS production, which might represent a general mechanism by which RALF and CrRLK1L function in signaling pathways.

scholarly journals Arabidopsis ABCG28 is required for the apical accumulation of reactive oxygen species in growing pollen tubes

2019 ◽  
Vol 116 (25) ◽  
pp. 12540-12549 ◽  
Author(s):  
Thanh Ha Thi Do ◽  
Hyunju Choi ◽  
Michael Palmgren ◽  
Enrico Martinoia ◽  
Jae-Ung Hwang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tip Growth ◽  
Ectopic Expression ◽  
Reactive Oxygen ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Oxygen Species ◽  
Ros Accumulation

Tip-focused accumulation of reactive oxygen species (ROS) is tightly associated with pollen tube growth and is thus critical for fertilization. However, it is unclear how tip-growing cells establish such specific ROS localization. Polyamines have been proposed to function in tip growth as precursors of the ROS, hydrogen peroxide. The ABC transporter AtABCG28 may regulate ROS status, as it contains multiple cysteine residues, a characteristic of proteins involved in ROS homeostasis. In this study, we found that AtABCG28 was specifically expressed in the mature pollen grains and pollen tubes. AtABCG28 was localized to secretory vesicles inside the pollen tube that moved toward and fused with the plasma membrane of the pollen tube tip. Knocking out AtABCG28 resulted in defective pollen tube growth, failure to localize polyamine and ROS to the growing pollen tube tip, and complete male sterility, whereas ectopic expression of this gene in root hair could recover ROS accumulation at the tip and improved the growth under high-pH conditions, which normally prevent ROS accumulation and tip growth. Together, these data suggest that AtABCG28 is critical for localizing polyamine and ROS at the growing tip. In addition, this function of AtABCG28 is likely to protect the pollen tube from the cytotoxicity of polyamine and contribute to the delivery of polyamine to the growing tip for incorporation into the expanding cell wall.

Reactive oxygen species produced by NADPH oxidase are involved in pollen tube growth

2007 ◽  
Vol 174 (4) ◽  
pp. 742-751 ◽  
Author(s):  
Martin Potocký ◽  
Mark A. Jones ◽  
Radek Bezvoda ◽  
Nicholas Smirnoff ◽  
Viktor Žárský
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Tube ◽  
Nadph Oxidase ◽  
Pollen Tube Growth ◽  
Reactive Oxygen ◽  
Tube Growth ◽  
Oxygen Species

PbrPOE21 inhibits pear pollen tube growth in vitro by altering apical reactive oxygen species content

Planta ◽  
2020 ◽  
Vol 252 (3) ◽  
Author(s):  
Ming Qian ◽  
Linlin Xu ◽  
Chao Tang ◽  
Hao Zhang ◽  
Hongru Gao ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Reactive Oxygen ◽  
Tube Growth ◽  
Oxygen Species ◽  
Reactive Oxygen Species Content

scholarly journals PAC1 regulates receptor tyrosine kinase transactivation in a reactive oxygen species-dependent manner

Peptides ◽  
2019 ◽  
Vol 120 ◽  
pp. 170017
Author(s):  
Terry W. Moody ◽  
Lingaku Lee ◽  
Tatiana Iordanskaia ◽  
Irene Ramos-Alvarez ◽  
Paola Moreno ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Tyrosine Kinase ◽  
Receptor Tyrosine Kinase ◽  
Reactive Oxygen ◽  
Dependent Manner ◽  
Oxygen Species

scholarly journals Blockage of Potassium Channel Inhibits Proliferation of Glioma Cells Via Increasing Reactive Oxygen Species

2014 ◽  
Vol 22 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Li Hu ◽  
Li-Li Li ◽  
Zhi-Guo Lin ◽  
Zhi-Chao Jiang ◽  
Hong-Xing Li ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Cycle ◽  
Glioma Cell ◽  
Glioma Cells ◽  
Reactive Oxygen ◽  
Dependent Manner ◽  
Oxygen Species ◽  
Brain Glioma ◽  
Protein Levels ◽  
Glioma Cell Proliferation

The potassium (K+) channel plays an important role in the cell cycle and proliferation of tumor cells, while its role in brain glioma cells and the signaling pathways remains unclear. We used tetraethylammonium (TEA), a nonselective antagonist of big conductance K+ channels, to block K+ channels in glioma cells, and antioxidant N-acetyl-l-cysteine (NAC) to inhibit production of intracellular reactive oxygen species (ROS). TEA showed an antiproliferation effect on C6 and U87 glioma cells in a time-dependent manner, which was accompanied by an increased intracellular ROS level. Antioxidant NAC pretreatment reversed TEA-mediated antiproliferation and restored ROS level. TEA treatment also caused significant increases in mRNA and protein levels of tumor-suppressor proteins p53 and p21, and the upregulation was attenuated by pretreatment of NAC. Our results suggest that K+ channel activity significantly contributes to brain glioma cell proliferation via increasing ROS, and it might be an upstream factor triggering the activation of the p53/p21Cip1-dependent signaling pathway, consequently leading to glioma cell cycle arrest.

scholarly journals Ethyl Rosmarinate Protects High Glucose-Induced Injury in Human Endothelial Cells

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3372 ◽  
Author(s):  
Yan-Hui Shen ◽  
Li-Ying Wang ◽  
Bao-Bao Zhang ◽  
Qi-Ming Hu ◽  
Pu Wang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Protective Effect ◽  
High Glucose ◽  
Reactive Oxygen ◽  
Annexin V ◽  
Ros Generation ◽  
Dependent Manner ◽  
Oxygen Species ◽  
Jnk Pathway

Ethyl rosmarinate (RAE) is one of the active constituents from Clinopodium chinense (Benth.) O. Kuntze, which is used for diabetic treatment in Chinese folk medicine. In this study, we investigated the protective effect of RAE on high glucose-induced injury in endothelial cells and explored its underlying mechanisms. Our results showed that both RAE and rosmarinic acid (RA) increased cell viability, decreased the production of reactive oxygen species (ROS), and attenuated high glucose-induced endothelial cells apoptosis in a dose-dependent manner, as evidenced by Hochest staining, Annexin V–FITC/PI double staining, and caspase-3 activity. RAE and RA both elevated Bcl-2 expression and reduced Bax expression, according to Western blot. We also found that LY294002 (phosphatidylinositol 3-kinase, or PI3K inhibitor) weakened the protective effect of RAE. In addition, PDTC (nuclear factor-κB, or NF-κB inhibitor) and SP600125 (c-Jun N-terminal kinase, or JNK inhibitor) could inhibit the apoptosis in endothelial cells caused by high glucose. Further, we demonstrated that RAE activated Akt, and the molecular docking analysis predicted that RAE showed more affinity with Akt than RA. Moreover, we found that RAE inhibited the activation of NF-κB and JNK. These results suggested that RAE protected endothelial cells from high glucose-induced apoptosis by alleviating reactive oxygen species (ROS) generation, and regulating the PI3K/Akt/Bcl-2 pathway, the NF-κB pathway, and the JNK pathway. In general, RAE showed greater potency than RA equivalent.

Sign in / Sign up

Export Citation Format

Share Document