scholarly journals The Associated With Carbon Conversion Rate and Source–Sink Enzyme Activity in Tomato Fruit Subjected to Water Stress and Potassium Application

2021 ◽  
Vol 12 ◽  
Author(s):  
Anrong Luo ◽  
Chenni Zhou ◽  
Jinliang Chen
Keyword(s):  
Enzyme Activity ◽  
Water Stress ◽  
Carbon Metabolism ◽  
Conversion Rate ◽  
Carbon Allocation ◽  
Higher Plants ◽  
Relative Rate ◽  
Tomato Fruit ◽  
Carbon Accumulation ◽  
Carbon Conversion

Carbon metabolism in higher plants is a basic physiological metabolism, and carbon allocation and conversion require the activity of various enzymes in metabolic processes that alter the content and overall composition of sugars in the sink organ. However, it is not known how various enzymes affect carbon metabolism when tomato plants are subjected to water stress or treated with potassium. Although the process of carbon metabolism is very complex, we used the carbon conversion rate to compare and analyze the enzyme activities related to sugar metabolism and find out which carbon conversion rate are the most important. Results showed that water stress and potassium increased carbon import flux in the fruit, which was beneficial to carbon accumulation. Water deficit increased the activity of sucrose synthase (SuSy) and starch phosphorylase (SP) and decreased the activity of sucrose phosphate synthase (SPS) and adenosine diphosphate glucose pyrophosphorylase (AGPase) in the source. Water stress increased the activity of acid invertase (AI), SuSy and SP but decreased the activity of AGPase in the sink. Potassium modified the balance of enzymes active in sugar and starch metabolism by increasing the activity of AI, SuSy, SPS and SP and significantly decreasing the activity of AGPase, resulting in increase of hexose. Canonical correlational analysis revealed that the carbon conversion rate was mainly affected by the relative rate of conversion of sucrose to fructose and glucose [p1(t)] and glucose to starch [p5m(t)]. SuSy and AGPase had the greatest effect on enzyme activity in the fruit; respectively regulated p1(t) and p5m(t).

scholarly journals Photosynthetic parameters and growth in seedlings of Bertholletia excelsa and Carapa guianensis in response to pre-acclimation to full sunlight and mild water stress

2014 ◽  
Vol 44 (1) ◽  
pp. 67-77 ◽  
Author(s):  
Gracilene Fernandes da Costa Azevedo
Keyword(s):  
Water Stress ◽  
Higher Plants ◽  
Field Capacity ◽  
Outdoor Exposure ◽  
Photosynthetic Parameters ◽  
Bertholletia Excelsa ◽  
Full Sunlight ◽  
Carapa Guianensis ◽  
Light Levels ◽  
Mild Water Stress

Light and water are important factors that may limit the growth and development of higher plants. The aim of this study was to evaluate photosynthetic parameters and growth in seedlings of Bertholletia excelsa and Carapa guianensis in response to pre-acclimation to full sunlight and mild water stress. I used six independent pre-acclimation treatments (0, 90 (11h15-12h45), 180 (10h30-13h30), 360 (09h00-15h00), 540 (07h30-16h30) and 720 min (06h00-18h00)) varying the time of exposure to full sunlight (PFS) during 30 days, followed by whole-day outdoor exposure for 120 days. Before PFS, the plants were kept in a greenhouse at low light levels (0.8 mol m-2 day-1). The PFS of 0 min corresponded to plants constantly kept under greenhouse conditions. From the beginning to the end of the experiment, each PFS treatment was submitted to two water regimes: moderate water stress (MWS, pre-dawn leaf water potential (ΨL) of -500 to -700 kPa) and without water stress (WWS, ΨL of -300 kPa, soil kept at field capacity). Plants under MWS received only a fraction of the amount of water applied to the well-watered ones. At the end of the 120-day-period under outdoor conditions, I evaluated light saturated photosynthesis (Amax), stomatal conductance (g s), transpiration (E) and plant growth. Both Amax and g s were higher for all plants under the PFS treatment. Stem diameter growth rate and Amax were higher for C. guianensis subjected to MWS than in well-watered plants. The contrary was true for B. excelsa. The growth of seedlings was enhanced by exposure to full sunlight for 180 minutes in both species. However, plants of B. excelsa were sensitive to moderate water stress. The higher photosynthetic rates and faster growth of C. guianensis under full sun and moderate water stress make this species a promissory candidate to be tested in reforestation programs.

Responses of two hybrid Populus clones to flooding, drought, and nitrogen availability. I. Morphology and growth

1992 ◽  
Vol 70 (11) ◽  
pp. 2265-2270 ◽  
Author(s):  
Zhijun Liu ◽  
Donald I. Dickmann
Keyword(s):  
Soil Moisture ◽  
Water Stress ◽  
Nitrogen Availability ◽  
Carbon Allocation ◽  
Hybrid Poplar ◽  
Leaf Expansion ◽  
Root Production ◽  
Leaf Initiation ◽  
Stem Biomass ◽  
Nitrogen Levels

Repeated progressive drought and flooding stress were imposed on hybrid poplar clones Populus × euramericana 'Eugenei', and Populus tristis × Populus balsamifera ‘Tristis’ grown in pots in a greenhouse under two nitrogen levels. In both clones the rate of leaf initiation was promoted only in high-N plants subjected to minimum water stress. Water stress alone did not retard the rate of leaf initiation, but it significantly reduced leaf expansion of 'Eugenei', whereas only flooding led to smaller leaves in 'Tristis'. The addition of N stimulated leaf expansion, leaf chlorophyll and N concentrations, and leaf and stem biomass production across soil moisture levels, but the greatest effect of N was associated with minimum water stress. High N altered carbon allocation towards the aboveground portions, leading to lower root to shoot ratios. High N also appeared to stimulate initiation of fine roots. Soil moisture determined the amount of biomass that accumulated in roots, with highest root production in well-watered pots and lowest in flooded pots, with the droughted treatment in between. Leaves became thinner as soil moisture decreased from flooding. Stem biomass of 'Tristis' declined more under flooding than under drought, whereas 'Eugenei' displayed a greater reduction of stem biomass in droughty than in flooded soil. Key words: water stress, nitrogen, leaf and root morphology, root to shoot ratio, biomass, Populus, flooding.

Photometric assay of maltose and maltose-forming enzyme activity by using 4-alpha-glucanotransferase (DPE2) from higher plants

2017 ◽  
Vol 532 ◽  
pp. 72-82 ◽  
Author(s):  
Julia Smirnova ◽  
Alisdair R. Fernie ◽  
Christian M.T. Spahn ◽  
Martin Steup
Keyword(s):  
Enzyme Activity ◽  
Higher Plants ◽  
Photometric Assay
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