scholarly journals Identification of Finely Mapped Quantitative Trait Locus and Candidate Gene Mining for the Three-Seeded Pod Trait in Soybean

2021 ◽  
Vol 12 ◽  
Author(s):  
Candong Li ◽  
Hongwei Jiang ◽  
Yingying Li ◽  
Chunyan Liu ◽  
Zhaoming Qi ◽  
...  
Keyword(s):  
Quantitative Trait Locus ◽  
Candidate Genes ◽  
Quantitative Trait ◽  
Recombinant Inbred Lines ◽  
Soybean Yield ◽  
Resource Group ◽  
Donor Parent ◽  
Important Trait ◽  
Trait Locus ◽  
Pod Number

The three-seeded pod number is an important trait that positively influences soybean yield. Soybean variety with increased three-seeded pod number contributes to the seed number/plant and higher yield. The candidate genes of the three-seeded pod may be the key for improving soybean yield. In this study, identification and validation of candidate genes for three-seeded pod has been carried out. First, a total of 36 quantitative trait locus (QTL) were detected from the investigation of recombinant inbred lines including 147 individuals derived from a cross between Charleston and Dongning 594 cultivars. Five consensus QTLs were integrated. Second, an introgressed line CSSL-182 carrying the target segment for the trait from the donor parent was selected to verify the consensus QTL based on its phenotype. Third, a secondary group was constructed by backcrossing with CSSL-182, and two QTLs were confirmed. There were a total of 162 genes in the two QTLs. The mining of candidate genes resulted in the annotation of eight genes with functions related to pod and seed sets. Finally, haplotype analysis and quantitative reverse transcriptase real-time PCR were carried to verify the candidate genes. Four of these genes had different haplotypes in the resource group, and the differences in the phenotype were highly significant. Moreover, the differences in the expression of the four genes during pod and seed development were also significant. These four genes were probably related to the development process underlying the three-seeded pod in soybean. Herein, we discuss the past and present studies related to the three-seeded pod trait in soybean.

scholarly journals Analysis of candidate genes for morphine preference quantitative trait locus Mop2

Neuroscience ◽  
2014 ◽  
Vol 277 ◽  
pp. 403-416 ◽  
Author(s):  
G.A. Doyle ◽  
C.L. Schwebel ◽  
S.E. Ruiz ◽  
A.D. Chou ◽  
A.T. Lai ◽  
...  
Keyword(s):  
Quantitative Trait Locus ◽  
Candidate Genes ◽  
Quantitative Trait ◽  
Trait Locus

scholarly journals Recessive Resistance Governed by a Major Quantitative Trait Locus Restricts Clover Yellow Vein Virus in Mechanically but Not Graft-Inoculated Cultivated Soybeans

2019 ◽  
Vol 32 (8) ◽  
pp. 1026-1037 ◽  
Author(s):  
Junya Abe ◽  
Yongzhi Wang ◽  
Tetsuya Yamada ◽  
Masako Sato ◽  
Takuya Ono ◽  
...  
Keyword(s):  
Quantitative Trait Locus ◽  
Quantitative Trait ◽  
Major Quantitative Trait Locus ◽  
Recombinant Inbred Lines ◽  
Systemic Infection ◽  
Yellow Vein ◽  
Systemic Spread ◽  
Clover Yellow Vein Virus ◽  
Trait Locus ◽  
Cultivated Soybean

Clover yellow vein virus (ClYVV) infects and causes disease in legume plants. However, here, we found that ClYVV isolate No. 30 (ClYVV-No.30) inefficiently multiplied or spread via cell-to-cell movement in mechanically inoculated leaves of a dozen soybean (Glycine max) cultivars and resulted in failure to spread systemically. Soybean plants also had a similar resistance phenotype against additional ClYVV isolates. In contrast, all but one of 24 tested accessions of wild soybeans (G. soja) were susceptible to ClYVV-No.30. Graft inoculation of cultivated soybean TK780 with ClYVV-No.30–infected wild soybean B01167 scion resulted in systemic infection of the cultivated soybean rootstock. This suggests that, upon mechanical inoculation, the cultivated soybean inhibits ClYVV-No.30, at infection steps prior to the systemic spread of the virus, via vascular systems. Systemic infection of all F1 plants from crossing between TK780 and B01167 and of 68 of 76 F2 plants with ClYVV-No.30 indicated recessive inheritance of the resistance. Further genetic analysis using 64 recombinant inbred lines between TK780 and B01167 detected one major quantitative trait locus, designated d-cv, for the resistance that was positioned in the linkage group D1b (chromosome 2). The mapped region on soybean genome suggests that d-cv is not an allele of the known resistance genes against soybean mosaic virus.

Quantitative trait locus analysis of multiple agronomic traits in the model legume Lotus japonicus

Genome ◽  
2007 ◽  
Vol 50 (7) ◽  
pp. 627-637 ◽  
Author(s):  
Takahiro Gondo ◽  
Shusei Sato ◽  
Kenji Okumura ◽  
Satoshi Tabata ◽  
Ryo Akashi ◽  
...  
Keyword(s):  
Quantitative Trait Locus ◽  
Quantitative Trait ◽  
Agronomic Traits ◽  
Recombinant Inbred Lines ◽  
Lotus Japonicus ◽  
Seed Mass ◽  
Chromosome 2 ◽  
Seed Traits ◽  
Model Legume ◽  
Trait Locus

The first quantitative trait locus (QTL) analysis of multiple agronomic traits in the model legume Lotus japonicus was performed with a population of recombinant inbred lines derived from Miyakojima MG-20 × Gifu B-129. Thirteen agronomic traits were evaluated in 2004 and 2005: traits of vegetative parts (plant height, stem thickness, leaf length, leaf width, plant regrowth, plant shape, and stem color), flowering traits (flowering time and degree), and pod and seed traits (pod length, pod width, seeds per pod, and seed mass). A total of 40 QTLs were detected that explained 5%–69% of total variation. The QTL that explained the most variation was that for stem color, which was detected in the same region of chromosome 2 in both years. Some QTLs were colocated, especially those for pod and seed traits. Seed mass QTLs were located at 5 locations that mapped to the corresponding genomic positions of equivalent QTLs in soybean, pea, chickpea, and mung bean. This study provides fundamental information for breeding of agronomically important legume crops.

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