scholarly journals Monitoring Coxiella burnetii Infection in Naturally Infected Dairy Sheep Flocks Throughout Four Lambing Seasons and Investigation of Viable Bacteria

2020 ◽  
Vol 7 ◽  
Author(s):  
Raquel Álvarez-Alonso ◽  
Ion I. Zendoia ◽  
Jesús F. Barandika ◽  
Isabel Jado ◽  
Ana Hurtado ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Dairy Sheep ◽  
Viable Bacteria

scholarly journals Coxiella burnetii seroprevalence and risk factors in sheep farmers and farm residents in The Netherlands

2013 ◽  
Vol 142 (6) ◽  
pp. 1231-1244 ◽  
Author(s):  
M. M. A. De LANGE ◽  
B. SCHIMMER ◽  
P. VELLEMA ◽  
J. L. A. HAUTVAST ◽  
P. M. SCHNEEBERGER ◽  
...  
Keyword(s):  
Risk Factors ◽  
The Netherlands ◽  
Coxiella Burnetii ◽  
High Frequency ◽  
Significant Risk ◽  
Infection Risk ◽  
Multivariate Logistic Regression ◽  
Dairy Sheep ◽  
Sheep Farm ◽  
Farm Residents

SUMMARYIn this study, Coxiella burnetii seroprevalence was assessed for dairy and non-dairy sheep farm residents in The Netherlands for 2009–2010. Risk factors for seropositivity were identified for non-dairy sheep farm residents. Participants completed farm-based and individual questionnaires. In addition, participants were tested for IgG and IgM C. burnetii antibodies using immunofluorescent assay. Risk factors were identified by univariate, multivariate logistic regression, and multivariate multilevel analyses. In dairy and non-dairy sheep farm residents, seroprevalence was 66·7% and 51·3%, respectively. Significant risk factors were cattle contact, high goat density near the farm, sheep supplied from two provinces, high frequency of refreshing stable bedding, farm started before 1990 and presence of the Blessumer breed. Most risk factors indicate current or past goat and cattle exposure, with limited factors involving sheep. Subtyping human, cattle, goat, and sheep C. burnetii strains might elucidate their role in the infection risk of sheep farm residents.

Kinetics of Coxiella burnetii excretion in a commercial dairy sheep flock after treatment with oxytetracycline

2010 ◽  
Vol 184 (2) ◽  
pp. 172-175 ◽  
Author(s):  
Ianire Astobiza ◽  
Jesús F. Barandika ◽  
Ana Hurtado ◽  
Ramón A. Juste ◽  
Ana L. García-Pérez
Keyword(s):  
Coxiella Burnetii ◽  
Sheep Flock ◽  
Dairy Sheep ◽  
Kinetics Of

scholarly journals Four-Year Evaluation of the Effect of Vaccination against Coxiella burnetii on Reduction of Animal Infection and Environmental Contamination in a Naturally Infected Dairy Sheep Flock

2011 ◽  
Vol 77 (20) ◽  
pp. 7405-7407 ◽  
Author(s):  
Ianire Astobiza ◽  
Jesús F. Barandika ◽  
Francisco Ruiz-Fons ◽  
Ana Hurtado ◽  
Inés Povedano ◽  
...  
Keyword(s):  
Phase I ◽  
Coxiella Burnetii ◽  
Environmental Contamination ◽  
Sheep Flock ◽  
Dairy Sheep ◽  
Content Type ◽  
Animal Infection

ABSTRACTVaccination is considered one of the best options for controllingCoxiella burnetiiinfection in livestock. The efficacy of a phase I vaccine was investigated over 4 years in a sheep flock with confirmedC. burnetiiinfection. Shedding was not detected in ewes and yearlings in the last 2 years, butC. burnetiistill persisted in the environment.

scholarly journals Short communication: Investigation of Coxiella burnetii occurrence in dairy sheep flocks by bulk-tank milk analysis and antibody level determination

2009 ◽  
Vol 92 (4) ◽  
pp. 1581-1584 ◽  
Author(s):  
A.L. García-Pérez ◽  
I. Astobiza ◽  
J.F. Barandika ◽  
R. Atxaerandio ◽  
A. Hurtado ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Antibody Level ◽  
Short Communication ◽  
Milk Analysis ◽  
Dairy Sheep ◽  
Bulk Tank Milk ◽  
Bulk Tank

scholarly journals Stable levels of Coxiella burnetii prevalence in dairy sheep flocks but changes in genotype distribution after a 10-year period in northern Spain

2018 ◽  
Vol 60 (1) ◽  
Author(s):  
Raquel Álvarez-Alonso ◽  
Jesús Felix Barandika ◽  
Francisco Ruiz-Fons ◽  
Ione Ortega-Araiztegi ◽  
Isabel Jado ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Genotype Distribution ◽  
Dairy Sheep ◽  
Northern Spain

scholarly journals Coxiella burnetii DNA, But Not Viable Bacteria, in Dairy Products in France

2013 ◽  
Vol 88 (4) ◽  
pp. 765-769 ◽  
Author(s):  
Carole Eldin ◽  
Aurélie Renvoisé ◽  
Didier Raoult ◽  
Emmanouil Angelakis
Keyword(s):  
Coxiella Burnetii ◽  
Dairy Products ◽  
Viable Bacteria

Coxiella burnetii shedding and environmental contamination at lambing in two highly naturally-infected dairy sheep flocks after vaccination

2011 ◽  
Vol 91 (3) ◽  
pp. e58-e63 ◽  
Author(s):  
I. Astobiza ◽  
J.F. Barandika ◽  
F. Ruiz-Fons ◽  
A. Hurtado ◽  
I. Povedano ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Environmental Contamination ◽  
Dairy Sheep

scholarly journals Nitric Oxide Partially Controls Coxiella burnetii Phase II Infection in Mouse Primary Macrophages

2003 ◽  
Vol 71 (3) ◽  
pp. 1225-1233 ◽  
Author(s):  
Dario S. Zamboni ◽  
Michel Rabinovitch
Keyword(s):  
Nitric Oxide ◽  
Phase Ii ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Bacterial Load ◽  
Peritoneal Macrophages ◽  
No Synthase ◽  
No Donor ◽  
Viable Bacteria ◽  
Ifn Γ

ABSTRACT In most primary or continuous cell cultures infected with the Q-fever agent Coxiella burnetii, bacteria are typically sheltered in phagolysosome-like, large replicative vacuoles (LRVs). We recently reported that only a small proportion of mouse peritoneal macrophages (PMΦ) infected with a nonvirulent, phase II strain of C. burnetii developed LRVs and that their relative bacterial load increased only slowly. In the majority of infected PMΦ, the bacteria were confined to the small vesicles. We show here that nitric oxide (NO) induced by the bacteria partially accounts for the restricted development of LRVs in primary macrophages. Thus, (i) PMΦ and bone marrow-derived macrophages (BMMΦ) challenged with phase II C. burnetii produced significant amounts of NO; (ii) the NO synthase inhibitors aminoguanidine and N-methyl-l-arginine reduced the production of NO and increased the frequency of LRVs (although the relative bacterial loads of individual LRVs did not change, the estimated loads per well increased appreciably); (iii) gamma interferon (IFN-γ) or the NO donor sodium nitroprusside, added to BMMΦ prior to or after infection, reduced the development and the relative bacterial loads of LRVs and lowered the yield of viable bacteria recovered from the cultures; and (iv) these effects of IFN-γ may not be entirely dependent on the production of NO since IFN-γ also controlled the infection in macrophages from inducible NO synthase knockout mice. It remains to be determined whether NO reduced the development of LRVs by acting directly on the bacteria; by acting on the traffic, fusion, or fission of cell vesicles; or by a combination of these mechanisms.

scholarly journals Circulation of Coxiella burnetii in a Naturally Infected Flock of Dairy Sheep: Shedding Dynamics, Environmental Contamination, and Genotype Diversity

2015 ◽  
Vol 81 (20) ◽  
pp. 7253-7260 ◽  
Author(s):  
A. Joulié ◽  
K. Laroucau ◽  
X. Bailly ◽  
M. Prigent ◽  
P. Gasqui ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Environmental Contamination ◽  
Q Fever ◽  
Management Strategies ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Dairy Sheep ◽  
Content Type ◽  
Ewe Lambs ◽  
Genotype Diversity

ABSTRACTQ fever is a worldwide zoonosis caused byCoxiella burnetii. Domestic ruminants are considered to be the main reservoir. Sheep, in particular, may frequently cause outbreaks in humans. Because within-flock circulation data are essential to implementing optimal management strategies, we performed a follow-up study of a naturally infected flock of dairy sheep. We aimed to (i) describeC. burnetiishedding dynamics by sampling vaginal mucus, feces, and milk, (ii) assess circulating strain diversity, and (iii) quantify barn environmental contamination. For 8 months, we sampled vaginal mucus and feces every 3 weeks from aborting and nonaborting ewes (n= 11 andn= 26, respectively); for lactating females, milk was obtained as well. We also sampled vaginal mucus from nine ewe lambs. Dust and air samples were collected every 3 and 6 weeks, respectively. All samples were screened using real-time PCR, and strongly positive samples were further analyzed using quantitative PCR. Vaginal and fecal samples with sufficient bacterial burdens were then genotyped by multiple-locus variable-number tandem-repeat analysis (MLVA) using 17 markers.C. burnetiiburdens were higher in vaginal mucus and feces than in milk, and they peaked in the first 3 weeks postabortion or postpartum. Primiparous females and aborting females tended to shedC. burnetiilonger and have higher bacterial burdens than nonaborting and multiparous females. Six genotype clusters were identified; they were independent of abortion status, and within-individual genotype diversity was observed.C. burnetiiwas also detected in air and dust samples. Further studies should determine whether the within-flock circulation dynamics observed here are generalizable.

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