scholarly journals Molecular Characterization of Hemoparasites and Hemoplasmas Infecting Domestic Cats of Southern India

2021 ◽  
Vol 7 ◽  
Author(s):  
Lanchalung Malangmei ◽  
Karapparambu Gopalan Ajith Kumar ◽  
Ashwathappa Nandini ◽  
Christophe Angeline Felicia Bora ◽  
Anju Varghese ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Microscopic Examination ◽  
18S Rrna Gene ◽  
Trypanosoma Evansi ◽  
Southern India ◽  
Nucleotide Sequencing ◽  
Rrna Gene ◽  
Gene Sequences ◽  
Hepatozoon Felis ◽  
Different Populations

In the present study, 111 blood samples were collected from apparently healthy cats belonging to four districts of Kerala, southern India, and they were investigated for the presence of hemoparasites and hemoplasmas by light microscopic examination and polymerase chain reaction (PCR). The microscopic examination of the Giemsa-stained blood smears did not reveal any parasites/organisms. However, PCR followed by nucleotide sequencing could detect 10 (9.01%) out of 111 samples infected with Hepatozoon felis, 3 (2.70%) with Cytauxzoon spp., and 10 (9.01%) with Mycoplasma spp. None of the samples revealed amplicons specific for the Babesia spp. and Trypanosoma evansi. The phylogenetic analysis of 18S ribosomal RNA (rRNA) gene sequences of H. felis revealed the existence of two different populations of H. felis circulating in the blood of infected cats. The phylogenetic tree was constructed based on 18S rRNA gene sequences of Cytauxzoon spp. and revealed that these isolates formed a distinct clade and do not cluster with any of the isolates from other countries. Among the 10 samples positive for Mycoplasma spp. infections, 7 were detected positive for Candidatus Mycoplasma haemominutum, two for Mycoplasma haemofelis, and one for Candidatus Mycoplasma turicensis. Phylogenetic analysis of 16S rRNA gene sequences of Mycoplasma spp. showed no distinct geographical grouping of the sequences. The sequences of M. haemofelis, Candidatus M. haemominutum, and Candidatus M. turicensis identified in the study clustered along with their respective isolates from around the world. To the best of our knowledge, this study forms the first report of molecular detection of Cytauxzoon spp. and Candidatus M. turicensis in cats from India.

Effect of Cell Culture on 18S rRNA Gene Sequences in the Cultural Course of Taxus chinensis Cells

2008 ◽  
Vol 63 (1-2) ◽  
pp. 127-132
Author(s):  
Fu Xiang ◽  
Long J. Yu ◽  
Wu Chen ◽  
Zhi Liu
Keyword(s):  
Cell Culture ◽  
Phylogenetic Analysis ◽  
18S Rrna ◽  
Relative Rate ◽  
18S Rrna Gene ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Taxus Chinensis ◽  
Gene Sequences ◽  
Parsimony Method

Cell culture is an effective technology for taxol production. This paper discusses the effect of Taxus cell cultures on the 18S rRNA gene sequences based on the phylogenetic analysis of cultured T. chinensis cells and related species. The phylogenetic tree is reconstructed using the maximum parsimony method and the relative rate test to test the hypothesis of a molecular clock. The phylogenetic analysis indicates that cell culture changes the phylogenetic position of cultured T. chinensis cells. More than that, the 18S rRNA gene of cultured T. chinensis cells has a faster rate of substitution than that of T. chinensis. With T. media as reference, the divergence time of the cultured T. chinensis cells is 7 Ma (million years) more than that of the T. chinensis cells based on the 18S rRNA gene sequences.

Phylogenetic Analysis Based on 18S rRNA Gene and matK Gene Sequences of Panax vietnamensis and Five Related Species

Planta Medica ◽  
2001 ◽  
Vol 67 (5) ◽  
pp. 461-465 ◽  
Author(s):  
Katsuko Komatsu ◽  
Shu Zhu ◽  
Hirotoshi Fushimi ◽  
Tran Kim Qui ◽  
Shaoqing Cai ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Related Species ◽  
18S Rrna ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Gene Sequences ◽  
Matk Gene

scholarly journals Phylogenetic Analysis of Indian Freshwater Pond Mussels Lamellidens corrianus and L. phenchooganjensis (Bivalvia: Unionidae) from the Upper Brahmaputra Basin of Assam, India

2021 ◽  
Vol 18 (1) ◽  
pp. 197-206
Author(s):  
Jyotish Sonowal ◽  
Pankaj Chetia ◽  
Devid Kardong
Keyword(s):  
Phylogenetic Analysis ◽  
18S Rrna ◽  
Gene Sequence ◽  
18S Rrna Gene ◽  
Phylogenetic Study ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Gene Sequences ◽  
Freshwater Molluscs ◽  
First Time

To update the present knowledge of freshwater molluscs, a phylogenetic analysis of two species of Indian pond mussels was carried out using amplified sequence of 18S rRNA gene. From the phylogenetic study of 18S rRNA gene sequencing, it was found that the Lamellidenscorrianus and L. phenchooganjensis are phylogenetically closely related to 18S rRNA gene sequences of other freshwater bivalve mussels belonging to the family Unionidae of order Unionida. Like other members of the Unionidae family, the two Lamellidensspp. showed monophyletic evolutionary lineage and shared a common ancestor. The result obtained from the phylogenetic analysis of Lamellidensspp. was significant as the 18S rRNA gene sequence of L. phenchooganjensis was submitted for the first time in the global nucleotide database (Genbank, NCBI). Similarly, the 18S rRNA gene sequence of L. corrianuswas also submitted to the database for the first time from this region having unique ecological niche. Therefore, the present study on phylogenetic analysis is a contribution to the global database of 18S rRNA gene sequences of freshwater mollusc, particularly from this part of the region.

scholarly journals The Termite Group I Phylum Is Highly Diverse and Widespread in the Environment

2007 ◽  
Vol 73 (20) ◽  
pp. 6682-6685 ◽  
Author(s):  
Daniel P. R. Herlemann ◽  
Oliver Geissinger ◽  
Andreas Brune
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Specific Primers ◽  
Environmental Distribution ◽  
Data Set ◽  
Group I

ABSTRACT The bacterial candidate phylum Termite Group I (TG-1) presently consists mostly of “Endomicrobia,” which are endosymbionts of flagellate protists occurring exclusively in the hindguts of termites and wood-feeding cockroaches. Here, we show that public databases contain many, mostly undocumented 16S rRNA gene sequences from other habitats that are affiliated with the TG-1 phylum but are only distantly related to “Endomicrobia.” Phylogenetic analysis of the expanded data set revealed several diverse and deeply branching lineages comprising clones from many different habitats. In addition, we designed specific primers to explore the diversity and environmental distribution of bacteria in the TG-1 phylum.

Phylogenetic analysis of Blastocystis isolates from different hosts based on the comparison of small-subunit rRNA gene sequences

2003 ◽  
Vol 126 (1) ◽  
pp. 119-123 ◽  
Author(s):  
Christophe Noël ◽  
Corinne Peyronnet ◽  
Delphine Gerbod ◽  
Virginia P Edgcomb ◽  
Pilar Delgado-Viscogliosi ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Gene Sequences ◽  
Small Subunit Rrna Gene

scholarly journals Asgard archaea are diverse, ubiquitous, and transcriptionally active microbes

2018 ◽  
Author(s):  
Mingwei Cai ◽  
Yang Liu ◽  
Zhichao Zhou ◽  
Yuchun Yang ◽  
Jie Pan ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Genomic Analysis ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Group D ◽  
Origin Of Eukaryotes

AbstractAsgard is a newly proposed archaeal superphylum. Phylogenetic position of Asgard archaea and its relationships to the origin of eukaryotes is attracting increasingly research interest. However, in-depth knowledge of their diversity, distribution, and activity of Asgard archaea remains limited. Here, we used phylogenetic analysis to cluster the publicly available Asgard archaeal 16S rRNA gene sequences into 13 subgroups, including five previously unknown subgroups. These lineages were widely distributed in anaerobic environments, with the majority of 16S rRNA gene sequences (92%) originating from sediment habitats. Co-occurrence analysis revealed potential relationships between Asgard, Bathyarchaeota, and Marine Benthic Group D archaea. Genomic analysis suggested that Asgard archaea are potentially mixotrophic microbes with divergent metabolic capabilities. Importantly, metatranscriptomics confirmed the versatile lifestyles of Lokiarchaeota and Thorarchaeota, which can fix CO2using the tetrahydromethanopterin Wood-Ljungdahl pathway, perform acetogenesis, and degrade organic matters. Overall, this study broadens the understandings of Asgard archaea ecology, and also provides the first evidence to support a transcriptionally active mixotrophic lifestyle of Asgard archaea, shedding light on the potential roles of these microorganisms in the global biogeochemical cycling.

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