scholarly journals An Improved Mesocotyl Elongation Assay for the Rapid Identification and Characterization of Strigolactone-Related Rice Mutants

Agronomy ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 208
Author(s):  
Suyash Patil ◽  
Syed Adeel Zafar ◽  
Muhammad Uzair ◽  
Jinfeng Zhao ◽  
Jingjing Fang ◽  
...  

Strigolactones (SLs) constitute an important class of plant hormones involved in diverse developmental activities in plant growth and host-parasite interaction. Although substantial progress has been made to understand this pathway, the mechanism of action is still elusive especially with its interaction with other phytohormones and downstream targets. Here we have utilized the negative role of strigolactones in rice (Oryza sativa L.) mesocotyl elongation as a morphological marker for the identification and characterization of new developmental mutants. We observed that deep sown seeds develop longer mesocotyl compared with the surface-grown seeds in the dark condition. Based on this observation, we have developed a method to access mesocotyl elongation consisting of the glass vessel and vermiculite as a growth media. Mesocotyl elongation in the modified deep sown system results in a many-fold increase compared to the surface-grown seeds in the dark condition. External application of SLs analog rac-GR24 rescued the elongated mesocotyl phenotype in the mutant defective in SLs synthesis but not the signaling mutant, demonstrating its applicability in the physiological experiments. The modified mesocotyl elongation assay can be used as a rapid method for characterization and identification of suppressors/enhancers and new developmental mutants in the SLs or its associated pathway saving a huge amount of time and space.

2009 ◽  
Vol 119 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Jiayu Wang ◽  
Tetsuya Nakazaki ◽  
Shuqian Chen ◽  
Wenfu Chen ◽  
Hiroki Saito ◽  
...  

2020 ◽  
pp. 82-85
Author(s):  
M.S. Nagendra ◽  
P. Selvaraju ◽  
R. Jerlin ◽  
K. Ganesamurthy ◽  
N. Senthil

Identification and characterization of crop varieties are crucial for ensuring the genetic purity of seeds. The present investigation was carried out to identify suitable chemical methods that are fast, reliable and easy for seed analysts, breeders and seed producers for identification of a variety. Twenty-five popular rice varieties in the seed supply chain of Tamil Nadu were subjected to phenol, modified phenol, NaOH, aroma, gelatinization temperature (alkali spreading value), GA3 and 2,4-D tests. The results of the experiment revealed that phenol and modified phenol tests changed the colour of TKM 9 and TRY 1 variety to brown but no colour change was observed in the variety I.W. Ponni variety. The NaOH test is useful for the identification of TKM 9 variety as it changed the colourless solution to red. GA3 and 2,4-D tests characterized the varieties based on the shoot growth into two and three groups respectively. However, all the variety lacked aroma and exhibited a high gelatinization temperature.


2008 ◽  
Vol 58 (2) ◽  
pp. 113-120 ◽  
Author(s):  
Sohei Kobayashi ◽  
Yoshimichi Fukuta ◽  
Hiroshi Takeda ◽  
Tadashi Sato ◽  
Mitsuru Osaki

2007 ◽  
Vol 115 (8) ◽  
pp. 1093-1100 ◽  
Author(s):  
Chang-Jie Yan ◽  
Ji-Hua Zhou ◽  
Song Yan ◽  
Feng Chen ◽  
Martin Yeboah ◽  
...  

1994 ◽  
Vol 35 (8) ◽  
pp. 1251-1256 ◽  
Author(s):  
Tetsuya Ookura ◽  
Masato Wada ◽  
Yoshikiyo Sakakibara ◽  
Kim Hyun Jeong ◽  
Issei Maruta ◽  
...  

2013 ◽  
Vol 35 (4) ◽  
pp. 491-499 ◽  
Author(s):  
Zhi-kun Wang ◽  
Yun-xiang Huang ◽  
Zheng-diao Miao ◽  
Zhi-yan Hu ◽  
Xin-zhang Song ◽  
...  

2015 ◽  
Vol 34 (9) ◽  
pp. 1579-1592 ◽  
Author(s):  
Wei Wang ◽  
Peng Zhao ◽  
Xue-mei Zhou ◽  
Han-xian Xiong ◽  
Meng-xiang Sun

Rice ◽  
2012 ◽  
Vol 5 (1) ◽  
Author(s):  
Hyun-Sook Lee ◽  
Kazuhiro Sasaki ◽  
Atsushi Higashitani ◽  
Sang-Nag Ahn ◽  
Tadashi Sato

Sign in / Sign up

Export Citation Format

Share Document