Histone Deacetylase Inhibitors and Microtubule Inhibitors Induce Apoptosis in Feline Luminal Mammary Carcinoma Cells

Feline mammary carcinoma (FMC) is the third most common type of neoplasia in cats, sharing similar epidemiological features with human breast cancer. In humans, histone deacetylases (HDACs) play an important role in the regulation of gene expression, with HDAC inhibitors (HDACis) disrupting gene expression and leading to cell death. In parallel, microtubules inhibitors (MTIs) interfere with the polymerization of microtubules, leading to cell cycle arrest and apoptosis. Although HDACis and MTIs are used in human cancer patients, in cats, data is scarce. In this study, we evaluated the antitumor properties of six HDACis (CI-994, panobinostat, SAHA, SBHA, scriptaid, and trichostatin A) and four MTIs (colchicine, nocodazole, paclitaxel, and vinblastine) using three FMC cell lines (CAT-MT, FMCp, and FMCm), and compared with the human breast cancer cell line (SK-BR-3). HDACis and MTIs exhibited dose-dependent antitumor effects in FMC cell lines, and for all inhibitors, the IC50 values were determined, with one feline cell line showing reduced susceptibility (FMCm). Immunoblot analysis confirmed an increase in the acetylation status of core histone protein HDAC3 and flow cytometry showed that HDACis and MTIs lead to cellular apoptosis. Overall, our study uncovers HDACis and MTIs as promising anti-cancer agents to treat FMCs.

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Profiles of Caspase Activation and Gene Expression in Human Breast Cancer Cell Line MCF-7, after Cyclophosphamide, Doxorubicin, 5-Fluorouracil (CDF) Multi-Drug Administration

JOURNAL OF HEALTH SCIENCE ◽

10.1248/jhs.56.81 ◽

2010 ◽

Vol 56 (1) ◽

pp. 81-87 ◽

Cited By ~ 2

Author(s):

Fumihiko Kugawa ◽

Akemichi Ueno

Keyword(s):

Breast Cancer ◽

Gene Expression ◽

Cell Line ◽

Breast Cancer Cell Line ◽

Human Breast Cancer ◽

Cancer Cell Line ◽

Human Breast Cancer Cell ◽

Human Breast ◽

Caspase Activation ◽

Mcf 7

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312 PUBLICATION Development, characterisation and gene expression profiling of a novel more invasive variant established from the human breast cancer cell line Hs 578T

European Journal of Cancer Supplements ◽

10.1016/s1359-6349(05)80610-x ◽

2005 ◽

Vol 3 (2) ◽

pp. 88

Keyword(s):

Breast Cancer ◽

Gene Expression ◽

Gene Expression Profiling ◽

Cell Line ◽

Breast Cancer Cell Line ◽

Expression Profiling ◽

Human Breast Cancer ◽

Cancer Cell Line ◽

Human Breast Cancer Cell ◽

Human Breast

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Abstract P3-06-35: Topoisomerase 1 gene copy aberrations in 52 human breast cancer cell lines and association to gene expression

10.1158/0008-5472.sabcs12-p3-06-35 ◽

2012 ◽

Cited By ~ 1

Author(s):

J Stenvang ◽

M Smid ◽

S Nielsen ◽

M Timmermans ◽

M Rømer ◽

...

Keyword(s):

Breast Cancer ◽

Gene Expression ◽

Cell Lines ◽

Breast Cancer Cell ◽

Human Breast Cancer ◽

Human Breast Cancer Cell ◽

Gene Copy ◽

Breast Cancer Cell Lines ◽

Human Breast ◽

Topoisomerase 1

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Assessment of Interactions between Cisplatin and Two Histone Deacetylase Inhibitors in MCF7, T47D and MDA-MB-231 Human Breast Cancer Cell Lines – An Isobolographic Analysis

PLoS ONE ◽

10.1371/journal.pone.0143013 ◽

2015 ◽

Vol 10 (11) ◽

pp. e0143013 ◽

Cited By ~ 14

Author(s):

Anna Wawruszak ◽

Jarogniew J. Luszczki ◽

Aneta Grabarska ◽

Ewelina Gumbarewicz ◽

Magdalena Dmoszynska-Graniczka ◽

...

Keyword(s):

Breast Cancer ◽

Histone Deacetylase ◽

Cell Lines ◽

Breast Cancer Cell ◽

Human Breast Cancer ◽

Histone Deacetylase Inhibitors ◽

Human Breast Cancer Cell ◽

Breast Cancer Cell Lines ◽

Human Breast ◽

Isobolographic Analysis

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Promoter methylation reduces C/EBPδ (CEBPD) gene expression in the SUM-52PE human breast cancer cell line and in primary breast tumors

Breast Cancer Research and Treatment ◽

10.1007/s10549-005-9061-3 ◽

2005 ◽

Vol 95 (2) ◽

pp. 161-170 ◽

Cited By ~ 32

Author(s):

D. Tang ◽

G.S. Sivko ◽

J.W. DeWille

Keyword(s):

Breast Cancer ◽

Gene Expression ◽

Cell Line ◽

Breast Cancer Cell Line ◽

Promoter Methylation ◽

Human Breast Cancer ◽

Cancer Cell Line ◽

Breast Tumors ◽

Human Breast Cancer Cell ◽

Human Breast

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Gene expression of cytokeratin 19 and its molecular detection in human breast cancer cell lines

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/j.jpba.2015.11.034 ◽

2016 ◽

Vol 120 ◽

pp. 25-31 ◽

Cited By ~ 1

Author(s):

Umaporn Uawisetwathana ◽

Ekkarat Rodpai ◽

Primchanien Moongkarndi

Keyword(s):

Breast Cancer ◽

Gene Expression ◽

Cell Lines ◽

Breast Cancer Cell ◽

Human Breast Cancer ◽

Molecular Detection ◽

Human Breast Cancer Cell ◽

Breast Cancer Cell Lines ◽

Cytokeratin 19 ◽

Human Breast

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Effects of estriol on growth, gene expression and estrogen response element activation in human breast cancer cell lines

Maturitas ◽

10.1016/j.maturitas.2014.01.004 ◽

2014 ◽

Vol 77 (4) ◽

pp. 336-343 ◽

Cited By ~ 18

Author(s):

Magnus Diller ◽

Susanne Schüler ◽

Stefan Buchholz ◽

Claus Lattrich ◽

Oliver Treeck ◽

...

Keyword(s):

Breast Cancer ◽

Gene Expression ◽

Cell Lines ◽

Breast Cancer Cell ◽

Human Breast Cancer ◽

Human Breast Cancer Cell ◽

Breast Cancer Cell Lines ◽

Estrogen Response Element ◽

Human Breast ◽

Estrogen Response

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Screening to Identify Commonly Used Chinese Herbs That AffectERBB2andESR1Gene Expression Using the Human Breast Cancer MCF-7 Cell Line

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2014/965486 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 8

Author(s):

Jen-Hwey Chiu ◽

Chun-Ju Chang ◽

Jing-Chong Wu ◽

Hui-Ju Liu ◽

Che-Sheng Wen ◽

...

Keyword(s):

Breast Cancer ◽

Gene Expression ◽

Protein Expression ◽

Cell Line ◽

Human Breast Cancer ◽

Luciferase Activity ◽

Chinese Herbs ◽

Herbal Extracts ◽

Human Breast ◽

Mcf 7

Aim. Our aim the was to screen the commonly used Chinese herbs in order to detect changes inERBB2andESR1gene expression using MCF-7 cells.Methods. Using the MCF-7 human breast cancer cell line, cell cytotoxicity and proliferation were evaluated by MTT and trypan blue exclusion assays, respectively. A luciferase reporter assay was established by transient transfecting MCF-7 cells with plasmids containing either theERBB2or theESR1promoter region linked to the luciferase gene. Chinese herbal extracts were used to treat the cells at 24 h after transfection, followed by measurement of their luciferase activity. The screening results were verified by Western blotting to measure HER2 and ERαprotein expression.Results. At concentrations that induced little cytotoxicity, thirteen single herbal extracts and five compound recipes were found to increase eitherERBB2orESR1luciferase activity. By Western blotting, Si-Wu-Tang, Kuan-Shin-Yin, and Suan-Tsao-Ren-Tang were found to increase either HER2 or ERαprotein expression. In addition,Ligusticum chuanxiongwas shown to have a great effect onERBB2gene expression and synergistically with estrogen to stimulate MCF-7 cell growth.Conclusion. Our results provide important information that should affect clinical treatment strategies among breast cancer patients who are receiving hormonal or targeted therapies.

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