scholarly journals Peptidomics Analysis of Virulent Peptides Involved in Streptococcus suis Pathogenesis

Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2480
Author(s):  
Chadaporn Chaiden ◽  
Janthima Jaresitthikunchai ◽  
Narumon Phaonakrop ◽  
Sittiruk Roytrakul ◽  
Anusak Kerdsin ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Culture Medium ◽  
Streptococcus Suis ◽  
Phosphate Transport ◽  
Zoonotic Pathogen ◽  
Host Environment ◽  
Streptococcal Disease ◽  
Simulated Environment ◽  
Suis Serotype ◽  
Highly Virulent

Streptococcus suis (S. suis) is a zoonotic pathogen causing severe streptococcal disease worldwide. S. suis infections in pigs and humans are frequently associated with the virulent S. suis serotype 2 (SS2). Though various virulence factors of S. suis have been proposed, most of them were not essentially accounted for in the experimental infections. In the present study, we compared the peptidomes of highly virulent SS2 and SS14 in humans, the swine causative serotypes SS7 and SS9, and the rarely reported serotypes SS25 and SS27, and they were cultured in a specified culture medium containing whole blood to simulate their natural host environment. LC-MS/MS could identify 22 unique peptides expressed in the six S. suis serotypes. Under the host-simulated environment, peptides from the ABC-type phosphate transport system (SSU05_1106) and 30S ribosomal protein S2 (rpsB) were detected in the peptidome of virulent SS2 and SS14. Therefore, we suggest that these two proteins or their derived peptides might be involved in the survival of S. suis when simulated with a blood environment.

PrsA contributes to Streptococcus suis serotype 2 pathogenicity by modulating secretion of selected virulence factors

2019 ◽  
Vol 236 ◽  
pp. 108375 ◽  
Author(s):  
Hanze Liu ◽  
Hao Fu ◽  
Xiaowu Jiang ◽  
Xiayi Liao ◽  
Min Yue ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Streptococcus Suis ◽  
Suis Serotype ◽  
Streptococcus Suis Serotype 2

scholarly journals The biological role of MutT in the pathogenesis of the zoonotic pathogen Streptococcus suis serotype 2

Virulence ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 1538-1549
Author(s):  
Quan Li ◽  
Xia Fei ◽  
Yuhang Zhang ◽  
Genglin Guo ◽  
Huoying Shi ◽  
...  
Keyword(s):  
Streptococcus Suis ◽  
Biological Role ◽  
Zoonotic Pathogen ◽  
Suis Serotype ◽  
Streptococcus Suis Serotype 2

scholarly journals Five Complete Genome Sequences Spanning the Dutch Streptococcus suis Serotype 2 and Serotype 9 Populations

2020 ◽  
Vol 9 (6) ◽  
Author(s):  
Boas C. L. van der Putten ◽  
Thomas J. Roodsant ◽  
Martin A. Haagmans ◽  
Constance Schultsz ◽  
Kees C. H. van der Ark
Keyword(s):  
Complete Genome ◽  
Streptococcus Suis ◽  
Genome Sequences ◽  
Content Type ◽  
Zoonotic Pathogen ◽  
Complete Genomes ◽  
Suis Serotype ◽  
Model Strain ◽  
Streptococcus Suis Serotype 2

The zoonotic pathogen Streptococcus suis can cause septicemia and meningitis in humans. We report five complete genomes of Streptococcus suis serotype 2 and serotype 9, covering the complete phylogeny of serotype 9 Dutch porcine isolates and zoonotic isolates. The isolates include the model strain S10 and the Dutch emerging zoonotic lineage.

Intranasal Vaccination With Multiple Virulence Factors Promotes Mucosal Clearance of Streptococcus suis Across Serotypes and Protects Against Meningitis in Mice

2019 ◽  
Vol 220 (10) ◽  
pp. 1679-1687 ◽  
Author(s):  
Xinxin Xing ◽  
Shuai Bi ◽  
Xin Fan ◽  
Meilin Jin ◽  
Wenjun Liu ◽  
...  
Keyword(s):  
T Cell ◽  
Virulence Factors ◽  
Mucosal Immunity ◽  
Natural Habitat ◽  
Streptococcus Suis ◽  
Critical Events ◽  
Cell Responses ◽  
Mucosal Site ◽  
Intranasal Vaccine ◽  
Suis Serotype

Abstract Background Streptococcus suis is an emerging zoonotic agent. Its natural habitat is the tonsils, which are the main portals of S. suis entry into the bloodstream of pigs. The remarkable variability of the bacteria and complex pathogenic mechanisms make the development of a vaccine a difficult task. Method Five conserved virulence factors involved in critical events of S. suis pathogenesis were combined and used as an intranasal vaccine (V5). The effect of V5 was investigated with intranasal and systemic challenge models. Results V5 induced antibody and T-cell responses at the mucosal site and systemically. The immunity promoted clearance of S. suis from the nasopharynx independent of S. suis serotypes and reduced lethality after systemic challenge with S. suis serotype 2. Moreover, mice that survived sepsis from intravenous infection developed meningitis, whereas none of these mice showed neuropathological symptoms after V5 receipt. Conclusion Intranasal immunization with multiple conserved virulence factors decreases S. suis colonization at the nasopharynx across serotypes and inhibits the dissemination of the bacteria in the host. The protective mucosal immunity effects would potentially reduce the S. suis reservoir and prevent S. suis disease in pigs.

scholarly journals Whole-Genome-Sequence of Streptococcus Suis LSM178 with a Novel ST1005 Characterized the Hyper Pathogenicity in Human Infection

2021 ◽  
Author(s):  
Yong Hu ◽  
Shiming Fu ◽  
Xingxing Dong ◽  
Lin Teng ◽  
Jinquan Li
Keyword(s):  
Genetic Information ◽  
Streptococcus Suis ◽  
Human Infection ◽  
Sequence Type ◽  
Whole Genome Sequence ◽  
Type I ◽  
Zoonotic Pathogen ◽  
The Public ◽  
Virulent Strains ◽  
Highly Virulent

Abstract Streptococcus suis (S. suis) has been well-recognized as a zoonotic pathogen worldwide gearing up a great risk to the public health. In this study, an S. suis LSM178 strain with serotype 2 and novel multi-locus sequence type of 1005, isolating from a patient, was interpreted for the pathogenicity by its genetic information. LSM178 was more efficiently invasive to Caco-2 cells than SC19 and P1/7. Phylogenetic analysis showed that LSM178 clustered with highly virulent strains including all human strains and epidemic strains. These serotype 2 S. suis from China shared exclusively the typical virulence characteristics including the maximum (95/96) virulent factors and type I-89 K Pathogenicity Island. Further, groups of genes were identified to distinguish these highly virulent strains from other generally virulent strains, emphasizing the key roles of genes modeling transcription, cell barrier, replication, recombination and repair on the high pathogenicity for highly virulent strains. Additionally, LSM178 contains a novel prophage conducive potentially to pathogenicity. These characters would contribute to deeply studying the pathogenic mechanism and virulence drift of human pathogenic S. suis.

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