scholarly journals Dietary Zinc Supplementation to Prevent Chronic Copper Poisoning in Sheep

Animals ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 227 ◽  
Author(s):  
Antonio Minervino ◽  
Marta López-Alonso ◽  
Raimundo Barrêto Júnior ◽  
Frederico Rodrigues ◽  
Carolina Araújo ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Optical Emission ◽  
Emission Spectrometry ◽  
Control Group ◽  
Treatment Groups ◽  
Inductively Coupled ◽  
Hepatic Copper ◽  
Cu Accumulation ◽  
The Difference ◽  
Hepatic Metallothionein

The aim of this study was to evaluate whether zinc (Zn) supplementation protects against hepatic copper (Cu) accumulation in copper-loaded sheep. Forty cross-bred lambs were assigned to five experimental groups. These included the control group (C) and four treatment groups that received Cu and/or Zn supplementation (dry matter (DM) basis) over 14 weeks, as follows: Cu (450 mg Cu/kg); Zn-35 (450 mg Cu + 35 mg Zn/kg); Zn-150 (450 mg Cu + 150 mg Zn/kg); and Zn-300 (450 mg Cu + 300 mg Zn/kg). Blood, liver, and bile samples were obtained for mineral determination by inductively coupled plasma optical emission spectrometry (ICP–OES). The hepatic metallothionein (MT) concentrations were also determined. At the end of the experiment, hepatic Cu concentrations were higher in all Cu-supplemented groups than in C. Hepatic Cu accumulation was lower in the groups receiving the Zn supplementation than in the Cu group, although the difference was only statistically significant (66%) in the Zn-300 group. The MT concentrations tended to be higher (almost two-fold) in the Zn groups (but were not dose related) than in the C and Cu groups, and they were related to hepatic Zn concentrations. Zn supplementation at 300 mg/kg DM is useful for preventing excessive hepatic Cu accumulation in sheep exposed to high dietary concentrations of Cu.

scholarly journals Competition for minerals (Zn, Mn, Fe, Cu) and Cd between sheep tapeworm (Moniezia expansa) and its definitive host sheep (Ovis aries)

2011 ◽  
Vol 48 (4) ◽  
pp. 237-243 ◽  
Author(s):  
I. Jankovská ◽  
D. Lukešová ◽  
J. Száková ◽  
I. Langrová ◽  
J. Vadlejch ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Optical Emission ◽  
Ovis Aries ◽  
Emission Spectrometry ◽  
Control Group ◽  
Inductively Coupled ◽  
Sheep Liver ◽  
Moniezia Expansa ◽  
Cd Exposure ◽  
Sheep Muscle

AbstractConcentrations of various essential and toxic elements (Zn, Mn, Fe, Cu and Cd) were analysed by inductively coupled plasma optical emission spectrometry (ICP-OES) in the sheep tapeworm (Moniezia expansa) and in different tissues of its host Ovis aries. The element concentrations of the cestode parasites were compared to different organs (liver, kidney, and muscle) of sheep that were exposed to experimental amounts of Cd (0.2 g of CdCl2 added to 10 ml of distilled water and administered orally to the sheep every day for a period of 1 week). All sheep were randomly divided into four groups; the first group (Cd) contained uninfected, Cd exposed sheep, and its control (group C) were uninfected and unexposed to Cd; the second group (TCd) contained infected, Cd exposed sheep, and its control (group CT) contained infected, unexposed sheep. The experimental Cd exposure resulted in significantly higher Mn concentrations in sheep tapeworms (10.0 mg/kg) than in sheep muscle (0.6 mg/kg) and kidney (0.8 mg/kg). The experimental Cd exposure also significantly decreased the Cu concentrations in sheep liver and muscle. Moreover Cd exposure decreased the Fe concentrations in sheep kidney but caused it to increase in sheep liver and muscle. Zinc concentrations showed no differences between groups (Cd, TCd, C, T) in any monitored sheep tissues. The article also discuss the effect of tapeworm infection on a significant decrease of Fe in sheep muscle, liver and kidneys, as well as a decrease in Cu levels of the muscles and liver. This mineral imbalance may contribute to various health problems such as osteoporosis, metabolic processes disorder, antioxidant (SOD) dysfunction etc.

scholarly journals Features of quantity elements and essential trace elements concentrations in saliva of young athletes

2015 ◽  
Vol 96 (2) ◽  
pp. 238-241 ◽  
Author(s):  
N A Troegubova ◽  
N V Rylova
Keyword(s):  
Trace Elements ◽  
Inductively Coupled Plasma ◽  
Optical Emission ◽  
Emission Spectrometry ◽  
Control Group ◽  
Young Athletes ◽  
Inductively Coupled ◽  
Essential Trace Elements ◽  
The Republic ◽  
Potassium Magnesium

Aim. To explore the concentrations of quantity elements and essential trace elements (calcium, potassium, magnesium, zinc, iron, chromium, and selenium) in saliva of young athletes.Methods. The study included 111 children aged 12-17 years who were allocated to three groups. The first group included 28 schoolchildren, the second group - 36 adolescents (students of the «Kasatka» swimming sports school) and the third group - 47 athletes (students of the Republican specialized youth sports school of Olympic reserve for grass hockey «Dynamo» of the Republic of Tatarstan). Mass spectrometry with inductively coupled plasma was performed on «Elan-9000» mass spectrometer; optical emission spectrometry with inductively coupled plasma was performed on «Optima 2000DV» optical emission spectrometer.Results. In young athletes of cyclic sports, there was a significant reduction of salivary calcium (by 1.16 times) and potassium (by 1.8 times) levels compared to control group. Salivary levels of magnesium, zinc, chromium and selenium were significantly increased in athletes.Conclusion. Revealed differences of quantity elements and essential trace elements salivary concentrations reflect the features of their metabolism at high physical and emotional stress.

Advances in sample digestion using microwave-ultraviolet radiations: phosphorus and sulfur determination in animal feed

2020 ◽  
Vol 16 ◽  
Author(s):  
Diogo L. R. Novo ◽  
Priscila T. Scaglioni ◽  
Rodrigo M. Pereira ◽  
Filipe S. Rondan ◽  
Gilberto S. Coelho Junior ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Animal Feed ◽  
Optical Emission ◽  
Emission Spectrometry ◽  
Official Method ◽  
Dissolved Carbon ◽  
Inductively Coupled ◽  
Relative Standard ◽  
Wide Range ◽  
Sulfur Determination

Background: Conventional analytical methods for phosphorus and sulfur determination in several matrices present normally analytical challenges regarding inaccuracy, detectability and waste generation. Objective: The main objective is proposing a green and feasible analytical method for phosphorus and sulfur determination in animal feed. Methods: Synergic effect between microwave and ultraviolet radiations during sample preparation was evaluated for the first time for the animal feed digestion associated with further phosphorus and sulfur determination by ion chromatography with conductivity detection. Dissolved carbon and residual acidity in final digests were used for the proposed method assessment. Phosphorus and sulfur values were compared with those obtained using conventional microwave-assisted wet digestion in closed vessels associated with inductively coupled plasma optical emission spectrometry and with those obtained using Association of Official Analytical Chemists International official method. Recovery tests and certified reference material analysis were performed. Animal feeds were analyzed using the proposed method. Results: Sample masses of 500 mg were efficiently digested using only 2 mol L -1 HNO3. The results obtained by the proposed method was not differing significantly (p > 0.05) from those obtained by the conventional and official methods. Suitable recoveries (from 94 to 99%), agreement with certified values (101 and 104%) and relative standard deviations (< 8%) were achieved. Phosphorus and sulfur content in commercial products varied in a wide range (P: 5,873 to 28,387 mg kg-1 and S: 2,165 to 4,501 mg kg-1 ). Conclusion: The proposed method is a green, safe, accurate, precise and sensitive alternative for animal feed quality control.

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