Background:
Conventional analytical methods for phosphorus and sulfur determination in several matrices
present normally analytical challenges regarding inaccuracy, detectability and waste generation.
Objective:
The main objective is proposing a green and feasible analytical method for phosphorus and sulfur
determination in animal feed.
Methods:
Synergic effect between microwave and ultraviolet radiations during sample preparation was evaluated for the
first time for the animal feed digestion associated with further phosphorus and sulfur determination by ion
chromatography with conductivity detection. Dissolved carbon and residual acidity in final digests were used for the
proposed method assessment. Phosphorus and sulfur values were compared with those obtained using conventional
microwave-assisted wet digestion in closed vessels associated with inductively coupled plasma optical emission
spectrometry and with those obtained using Association of Official Analytical Chemists International official method.
Recovery tests and certified reference material analysis were performed. Animal feeds were analyzed using the proposed
method.
Results:
Sample masses of 500 mg were efficiently digested using only 2 mol L
-1 HNO3. The results obtained by the
proposed method was not differing significantly (p > 0.05) from those obtained by the conventional and official methods.
Suitable recoveries (from 94 to 99%), agreement with certified values (101 and 104%) and relative standard deviations (<
8%) were achieved. Phosphorus and sulfur content in commercial products varied in a wide range (P: 5,873 to 28,387 mg
kg-1
and S: 2,165 to 4,501 mg kg-1
).
Conclusion:
The proposed method is a green, safe, accurate, precise and sensitive alternative for animal feed quality
control.
Ion chromatography coupled to mass spectrometry as a powerful technique for halogens and sulfur determination in egg powder and its fractions
