scholarly journals GSTO2 Isoforms Participate in the Oxidative Regulation of the Plasmalemma in Eutherian Spermatozoa during Capacitation

Antioxidants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 601 ◽  
Author(s):  
Lauren E. Hamilton ◽  
Michal Zigo ◽  
Jiude Mao ◽  
Wei Xu ◽  
Peter Sutovsky ◽  
...  
Keyword(s):  
Membrane Lipid ◽  
Surface Proteins ◽  
Sperm Function ◽  
Oxygen Species ◽  
Glutathione S Transferase ◽  
Isolation And Identification ◽  
Vitro Fertilization ◽  
Epididymal Maturation ◽  
Oxidative Regulation

In addition to perinuclear theca anchored glutathione-s-transferase omega 2 (GSTO2), whose function is to participate in sperm nuclear decondensation during fertilization (Biol Reprod. 2019, 101:368–376), we herein provide evidence that GSTO2 is acquired on the sperm plasmalemma during epididymal maturation. This novel membrane localization was reinforced by the isolation and identification of biotin-conjugated surface proteins from ejaculated and capacitated boar and mouse spermatozoa, prompting us to hypothesize that GSTO2 has an oxidative/reductive role in regulating sperm function during capacitation. Utilizing an inhibitor specific to the active site of GSTO2 in spermatozoa, inhibition of this enzyme led to a decrease in tyrosine phosphorylation late in the capacitation process, followed by an expected decrease in acrosome exocytosis and motility. These changes were accompanied by an increase in reactive oxygen species (ROS) levels and membrane lipid peroxidation and culminated in a significant decrease in the percentage of oocytes successfully penetrated by sperm during in vitro fertilization. We conclude that GSTO2 participates in the regulation of sperm function during capacitation, most likely through protection against oxidative stress on the sperm surface.

scholarly journals A Potential Relationship Between Estrogen Receptors Polymorphisms, Sperm Function and in vitro Fertilization Success: A Preliminary Study*

2021 ◽  
Vol 75 ◽  
pp. 304-317
Author(s):  
Joanna Talarczyk-Desole ◽  
Mirosław Andrusiewicz ◽  
Małgorzata Chmielewska ◽  
Anna Berger ◽  
Leszek Pawelczyk ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Estrogen Receptors ◽  
Strong Association ◽  
Fertilization Success ◽  
World Health ◽  
Semen Parameters ◽  
Sperm Function ◽  
Allele Distribution ◽  
Vitro Fertilization

Background: Estrogen receptor 1 (ESR1) and 2 (ESR2) play an important role in regulating fertility in the human reproductive system. Polymorphisms of these receptor genes have been implicated in male infertility in both Chinese and Caucasian populations. However, studies have produced inconsistent results. Spermatozoa defects that result in conception deficiencies could be related to estrogens, their receptors, or genes involved in estrogen-related pathways. This study aims to explore the potential association between the ESR1 and the ESR2 polymorphisms in relation to semen parameters of Caucasian males as well as fertilization success. Materials/Methods: A total of 116 males were included in this study. Forty couples underwent conventional in vitro fertilization, while 76 couples were treated by intracytoplasmic sperm injection. Standard semen analyses were performed according to the World Health Organization criteria. Polymerase chain reaction and restriction fragment length polymorphisms were used to determine genotype and allele distributions. Results: A strong association between the ESR1 rs2234693 recognized by PvuII enzyme, genotype/allele distribution and fertilization success was shown. The T allele occurrence was significantly lower in the case of fertilization failure (p = 0.02). Additionally, the TT genotype was absent in the same group (p=0.02). In the case of the remaining analyzed polymorphisms, little to no interdependence of genotype/allele distribution and fertilization success was noted. Conclusions: Apart from ESR1 rs2234693, the study failed to demonstrate that fertilization success was associated with the selected polymorphisms. In most cases, we did not discover a relationship between both estrogen receptors polymorphisms and sperm function.

Sperm Performance Better on Diamond than on Polystyrene

2013 ◽  
Vol 1511 ◽  
Author(s):  
Andrei P. Sommer ◽  
Dan Zhu ◽  
Friedrich Gagsteiger ◽  
Hans-Jörg Fecht
Keyword(s):  
Embryonal Carcinoma ◽  
Petri Dish ◽  
Carcinoma Cells ◽  
Oxygen Species ◽  
Embryonal Carcinoma Cells ◽  
Vitro Fertilization ◽  
Polystyrene Petri Dish ◽  
Petri Dishes ◽  
New Generation

ABSTRACTRecently we postulated that polystyrene Petri dishes become soft when in contact with an aqueous milieu. Specifically, we assumed that the effect is restricted to a superficial nanolayer, a condition presumably favoring the establishment of a stable nanolayer of reactive oxygen species (ROS) at the liquid/solid-interface. Cells are known to be hypersensitive to ROS. Previously we used P19 mouse embryonal carcinoma cells and systematically analyzed their capability to climb different substrates placed vertically into a Petri dish. The worst and best performance was found on polystyrene (Petri dish material) and nanocrystalline diamond, respectively. Polystyrene Petri dishes are today standard in laboratories conducting in vitro fertilization (IVF). Here we proceed and extend the investigation to human spermatozoa and show that their performance (vitality) on polystyrene Petri dishes is low compared to that on diamond Petri dishes. This work may propel further research and inspire the development of a new generation of cell-friendly Petri dishes.

Pentoxifylline is not useful in enhancing sperm function in cases with previous in vitro fertilization failure*

1993 ◽  
Vol 59 (1) ◽  
pp. 210-215 ◽  
Author(s):  
Herman Tournaye ◽  
Ronny Janssens ◽  
Michel Camus ◽  
Catherine Staessen ◽  
Paul Devroey ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Sperm Function ◽  
Fertilization Failure ◽  
Vitro Fertilization

The influence of sperm concentration, length of the gamete co-culture and the evolution of different sperm parameters on the in vitro fertilization of prepubertal goat oocytes

Zygote ◽  
2010 ◽  
Vol 18 (4) ◽  
pp. 345-355 ◽  
Author(s):  
M.J. Palomo ◽  
T. Mogas ◽  
D. Izquierdo ◽  
M.T. Paramio
Keyword(s):  
Acrosome Reaction ◽  
Penetration Rate ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Oxygen Species ◽  
Vitro Fertilization ◽  
Rate Of Penetration ◽  
Sperm Penetration ◽  
Kinetics Of

SummaryThe aims of the present study were: (1) to evaluate the influence of sperm concentration (ranging from 0.5 × 106 to 4 × 106 spermatozoa/ml) and length of the gamete co-incubation time (2, 4, 6, 8, 10, 12, 16, 20, 24 or 28 h) on in vitro fertilization (IVF), assessing the sperm penetration rate; (2) to investigate the kinetics of different semen parameters as motility, viability and acrosome status during the co-culture period; and (3) to analyse the effect of the presence of cumulus–oocytes complexes (COCs) on these parameters. To achieve these objectives, several experiments were carried out using in vitro matured oocytes from prepubertal goats. The main findings of this work are that: (1) in our conditions, the optimum sperm concentration is 4 × 106 sperm/ml, as this sperm:oocyte ratio (approximately 28,000) allowed us to obtain the highest penetration rate, without increasing polyspermy incidence; (2) the highest percentage of viable acrosome-reacted spermatozoa is observed between 8–12 h of gamete co-culture, while the penetration rate is maximum at 12 h of co-incubation; and (3) the presence of COCs seems to favour the acrosome reaction of free spermatozoa on IVF medium, but not significantly. In conclusion, we suggest that a gamete co-incubation for 12–14 h, with a concentration of 4 × 106 sperm/ml, would be sufficient to obtain the highest rate of penetration, reducing the exposure of oocytes to high levels of reactive oxygen species produced by spermatozoa, especially when a high sperm concentration is used to increase the caprine IVF outcome.

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