scholarly journals Characterization of the Age-Dependent Changes in Antioxidant Defenses and Protein’s Sulfhydryl/Carbonyl Stress in Human Follicular Fluid

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 927
Author(s):  
Alice Luddi ◽  
Laura Governini ◽  
Angela Capaldo ◽  
Giovanna Campanella ◽  
Vincenzo De Leo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Follicular Fluid ◽  
Structural Changes ◽  
Early Embryo ◽  
Antioxidant Defenses ◽  
Carbonyl Stress ◽  
2D Electrophoresis ◽  
Human Follicular Fluid ◽  
Vitro Fertilization

The oxidative stress, characterized by the imbalance between pro-oxidants and antioxidants molecules, seems to be involved in the pathogenesis of female subfertility. In particular, the presence of different markers of oxidative stress has been reported in human follicular fluid (FF) surrounding oocytes. Based on its distinctive composition and on the close proximity to the oocyte, FF creates a unique microenvironment having a direct impact on oocyte quality, implantation, and early embryo development. An imbalance in reactive oxygen species (ROS) production in ovarian follicular fluid may have a negative effect on these processes and, as a consequence, on female fertility. Therefore, the aim of this study was to evaluate the redox state of the FF through various methodological approaches. By means of 2D-electrophoresis we demonstrated that the main structural changes occurring in the proteins of the follicular fluid of normovulatory women were correlated to the age of the patients and to the antioxidant defenses present in the FF. Measurement of these parameters could have clinical relevance, since the assessment of the oxidative stress rate may be helpful in evaluating in vitro fertilization potential.

Physiological levels of immunoreactive ANH-like peptides in human follicular fluid

1989 ◽  
Vol 121 (4) ◽  
pp. 578-580 ◽  
Author(s):  
J. A. Sundsfjord ◽  
F. Forsdahl ◽  
G. Thibault
Keyword(s):  
Fluid Dynamics ◽  
In Vitro Fertilization ◽  
Young Women ◽  
Follicular Fluid ◽  
Physiological Role ◽  
Plasma Samples ◽  
Human Follicular Fluid ◽  
Vitro Fertilization ◽  
Follicular Maturation

Abstract. The concentrations of immunoreactive C-terminal (ANH-(99-126)) and N-terminal (ANH-(1-98)) portions of pro-ANH were measured in follicular fluid and plasma samples from 9 young women undergoing in vitro fertilization. ANH-(99-126) and ANH-(1-98)-like immunoreactivity levels in plasma were 6.0–25.4 (mean 12.2) pmol/1 and 184–427 (mean 300) pmol/1, respectively, whereas the corresponding levels in follicular fluid were 3.8–8.0 (mean 4.9) pmol/1 and 169–385 (mean 262) pmol/1. The concentrations of both ANH-like peptides were consistently lower (p < 0.01) in the follicular fluid than in the matched plasma samples, but within the variation found in plasma controls. It is concluded that ANH-like peptides in the follicular fluid, whether secreted locally or derived from circulating ANH, might play a physiological role in the biosynthesis of ovarian steroid hormones or follicular maturation and fluid dynamics.

scholarly journals From “Every Day” Hormonal to Oxidative Stress Biomarkers in Blood and Follicular Fluid, to Embryo Quality and Pregnancy Success?

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Katarzyna Olszak-Wąsik ◽  
Anna Bednarska-Czerwińska ◽  
Anita Olejek ◽  
Andrzej Tukiendorf
Keyword(s):  
Oxidative Stress ◽  
Follicular Fluid ◽  
Embryo Quality ◽  
R Package ◽  
Single Embryo Transfer ◽  
Oxidative Stress Biomarkers ◽  
Oocyte Growth ◽  
Vitro Fertilization ◽  
Stress Markers

Background. Oxidative stress appears to be involved in oocyte growth and maturation that when impaired results in poor embryo quality and lower potential to implant. The biochemical microenvironment of the oocyte (follicular fluid (FF)) consists of hormones and other various substances regulating the balance between oxidants and antioxidants. Aim. The aim of this study was to examine the possible impact of selected biomarkers (“every day,” hormonal biomarkers, enzymatic and nonenzymatic antioxidants, and also oxidative stress markers) in serum and FF, on embryo quality and pregnancy success in infertile women undergoing infertility treatment. Methods. All 53 patients, mean age 34.7±4.1 years, with serum AMH level≥0.7 ng/mL, were diagnosed with idiopathic infertility. They were stimulated in short antagonist protocol, followed by in vitro fertilization (IVF-ICSI intracytoplasmatic sperm injection) and a single embryo transfer. Follicular fluid was aspirated from the first mature follicle. In statistical analyses the R software was used, then all data was assessed with the Shapiro-Wilk test, logistic regression, and later the receiver operating characteristic (ROC) curve was applied using “pROC” R package. Results. We did not observe any correlation between AMH and embryo quality and pregnancy rate. Statistically significant results were only found for biomarkers examined in follicular fluid. Greater levels of GPX in FF were associated with the increased chance of producing a high quality embryo (the optimal cut-off concentration was established at over 450 lU/L.) Regarding pregnancy success, increasing levels of GR (cut-off at 21 IU/L), CuZnSOD (cut-off at 9NU/mL), and GST (cut-off at 2.5 IU/L) resulted in lower chances of a successful pregnancy. Conclusion. Our results indicate that FF markers may have some advantages in predicting embryo quality and pregnancy over AMH. The GPX system seems to be mostly related to embryo quality and pregnancy.

Proteomic analysis of human follicular fluid in poor ovarian responders during in vitro fertilization

PROTEOMICS ◽  
2017 ◽  
Vol 17 (6) ◽  
pp. 1600333 ◽  
Author(s):  
Jae Won Oh ◽  
Seul Ki Kim ◽  
Kyung-Cho Cho ◽  
Min-Sik Kim ◽  
Chang Suk Suh ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Proteomic Analysis ◽  
Human Follicular Fluid ◽  
Vitro Fertilization ◽  
Poor Ovarian Responders
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