scholarly journals Fibrin with Laminin-Nidogen Reduces Fibrosis and Improves Soft Palate Regeneration Following Palatal Injury

Biomolecules ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1547
Author(s):  
Doris H. Rosero Salazar ◽  
René E. M. van Rheden ◽  
Manon van Hulzen ◽  
Paola L. Carvajal Monroy ◽  
Frank A. D. T. G. Wagener ◽  
...  
Keyword(s):  
Soft Palate ◽  
Muscle Regeneration ◽  
Smooth Muscle Actin ◽  
Collagen Deposition ◽  
Muscle Actin ◽  
Muscle Injuries ◽  
Promising Strategy ◽  
Homeobox Protein ◽  
Old Rats ◽  
Palate Repair

This study aimed to analyze the effects of fibrin constructs enhanced with laminin-nidogen, implanted in the wounded rat soft palate. Fibrin constructs with and without laminin-nidogen were implanted in 1 mm excisional wounds in the soft palate of 9-week-old rats and compared with the wounded soft palate without implantation. Collagen deposition and myofiber formation were analyzed at days 3, 7, 28 and 56 after wounding by histochemistry. In addition, immune staining was performed for a-smooth muscle actin (a-SMA), myosin heavy chain (MyHC) and paired homeobox protein 7 (Pax7). At day 56, collagen areas were smaller in both implant groups (31.25 ± 7.73% fibrin only and 21.11 ± 6.06% fibrin with laminin-nidogen)) compared to the empty wounds (38.25 ± 8.89%, p < 0.05). Moreover, the collagen area in the fibrin with laminin-nidogen group was smaller than in the fibrin only group (p ˂ 0.05). The areas of myofiber formation in the fibrin only group (31.77 ± 10.81%) and fibrin with laminin-nidogen group (43.13 ± 10.39%) were larger than in the empty wounds (28.10 ± 11.68%, p ˂ 0.05). Fibrin-based constructs with laminin-nidogen reduce fibrosis and improve muscle regeneration in the wounded soft palate. This is a promising strategy to enhance cleft soft palate repair and other severe muscle injuries.

Antifibrotic effects of suramin in injured skeletal muscle after laceration

2003 ◽  
Vol 95 (2) ◽  
pp. 771-780 ◽  
Author(s):  
Yi-Sheng Chan ◽  
Yong Li ◽  
William Foster ◽  
Takashi Horaguchi ◽  
George Somogyi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Growth Factor ◽  
Muscle Regeneration ◽  
Sports Medicine ◽  
Transforming Growth Factor ◽  
Smooth Muscle Actin ◽  
Healing Process ◽  
Muscle Injuries

Muscle injuries are very common in traumatology and sports medicine. Although muscle tissue can regenerate postinjury, the healing process is slow and often incomplete; complete recovery after skeletal muscle injury is hindered by fibrosis. Our studies have shown that decreased fibrosis could improve muscle healing. Suramin has been found to inhibit transforming growth factor (TGF)-β1 expression by competitively binding to the growth factor receptor. We conducted a series of tests to determine the antifibrotic effects of suramin on muscle laceration injuries. Our results demonstrate that suramin (50 μg/ml) can effectively decrease fibroblast proliferation and fibrotic-protein expression (α-smooth muscle actin) in vitro. In vivo, direct injection of suramin (2.5 mg) into injured murine muscle resulted in effective inhibition of muscle fibrosis and enhanced muscle regeneration, which led to efficient functional muscle recovery. These results support our hypothesis that prevention of fibrosis could enhance muscle regeneration, thereby facilitating more efficient muscle healing. This study could significantly contribute to the development of strategies to promote efficient muscle healing and functional recovery.

??-SMOOTH MUSCLE ACTIN AND COLLAGEN DEPOSITION IN DYSFUNCTIONAL RENAL TRANSPLANTS

1997 ◽  
Vol 63 (1) ◽  
pp. 156-158 ◽  
Author(s):  
Yoo-Joung Ko ◽  
Linda Sugar ◽  
Jeffrey Zaltzman ◽  
Leendert C. Paul
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Collagen Deposition ◽  
Muscle Actin ◽  
Renal Transplants

scholarly journals POSSIBILITIES OF REGENERATION OF THE MUSCLES OF THE SOFT PALATE DURING ITS NONUNION DEPENDING ON THE MYOGENIC POTENTIAL OF STEM CELLS. Review

2020 ◽  
Vol 16 (3) ◽  
pp. 63-71
Author(s):  
L.V. Kharkov ◽  
R.I. Egorov
Keyword(s):  
Stem Cells ◽  
Cell Therapy ◽  
Satellite Cells ◽  
Soft Palate ◽  
Muscle Regeneration ◽  
Adult Stem Cells ◽  
Future Research ◽  
Congenital Defects ◽  
Muscle Injuries ◽  
Myogenic Potential

Relevance. Today there are more than 150 methods for eliminating congenital defects of the hard and soft palate. However, these techniques do not always lead to high functional results, which leads to repeated surgical interventions and long-term speech therapy rehabilitation. Therefore, there is a problem with the prognosis of such treatment. The search for a marker for assessing the prognosis of surgical intervention is relevant. One of these markers may be the state of the myogenic potential of stem cells. Objective: to analyze the possibility of preliminary assessment of muscle regeneration, depending on the myogenic potential of stem cells, in order to increase the effectiveness of treatment of children with non-union of the soft palate. Method. An analytical review of the literature on keywords from the scientometric databases PubMed, Scopus, Web of Science. Results. Satellite cells represent an adequate system model for studying the biology of adult stem cells. Satellite cells can be considered candidates for cell therapy in muscle regeneration. First, they are one of the most abundant and most accessible cells in our body. Secondly, there is a panel of specific markers that can be used to isolate satellite cells. Third, satellite cells are localized within clear boundaries of the anatomical niche, and signaling mechanisms are currently being studied. Fourth, there is the possibility of recreating muscle injuries in which satellite cells can be studied. Future research aimed at increasing the purification of satellite cells so as to maintain their low differentiation, increase the engraftment potential, as well as new approaches aimed at obtaining satellite cells from iPS cells, will help accelerate the progress and development of drugs for cell therapy in the treatment of muscle degenerative diseases. Conclusions. The data on the myogenic potential of stem cells, in muscle regeneration, obtained on satellite cell models, can be used to increase the effectiveness of the treatment of children with nonunion of the soft palate.

scholarly journals Influence of Laminin Coating on the Autologous In Vivo Recellularization of Decellularized Vascular Protheses

Materials ◽  
2019 ◽  
Vol 12 (20) ◽  
pp. 3351 ◽  
Author(s):  
Mahfuza Toshmatova ◽  
Sentaro Nakanishi ◽  
Yukiharu Sugimura ◽  
Vera Schmidt ◽  
Artur Lichtenberg ◽  
...  
Keyword(s):  
Inflammatory Cell ◽  
Smooth Muscle Actin ◽  
Muscle Actin ◽  
Similar Extent ◽  
Alpha Smooth Muscle Actin ◽  
Promising Strategy ◽  
Biological Implants ◽  
Significant Acceleration ◽  
The Media

Decellularization of non-autologous biological implants reduces the immune response against foreign tissue. Striving for in vivo repopulation of aortic prostheses with autologous cells, thereby improving the graft biocompatibility, we examined surface coating with laminin in a standardized rat implantation model. Detergent-decellularized aortic grafts from donor rats (n = 37) were coated with laminin and systemically implanted into Wistar rats. Uncoated implants served as controls. Implant re-colonization and remodeling were examined by scanning electron microscopy (n = 10), histology and immunohistology (n = 18). Laminin coating persisted over eight weeks. Two weeks after implantation, no relevant neoendothelium formation was observed, whereas it was covering the whole grafts after eight weeks, with a significant acceleration in the laminin group (p = 0.0048). Remarkably, the intima-to-media ratio, indicating adverse hyperplasia, was significantly diminished in the laminin group (p = 0.0149). No intergroup difference was detected in terms of medial recellularization (p = 0.2577). Alpha-smooth muscle actin-positive cells originating from the adventitial surface invaded the media in both groups to a similar extent. The amount of calcifying hydroxyapatite deposition in the intima and the media did not differ between the groups. Inflammatory cell markers (CD3 and CD68) proved negative in coated as well as uncoated decellularized implants. The coating of decellularized aortic implants with bioactive laminin caused an acceleration of the autologous recellularization and a reduction of the intima hyperplasia. Thereby, laminin coating seems to be a promising strategy to enhance the biocompatibility of tissue-engineered vascular implants.

scholarly journals Preoperatively diagnosed gastric collision tumor with mixed adenocarcinoma and gastrointestinal stromal tumor: a case report and literature review

2021 ◽  
Author(s):  
Kunihiko Matsuno ◽  
Yoshikazu Kanazawa ◽  
Daisuke Kakinuma ◽  
Nobutoshi Hagiwara ◽  
Fumihiko Ando ◽  
...  
Keyword(s):  
Gastrointestinal Stromal Tumor ◽  
Distal Gastrectomy ◽  
Submucosal Tumor ◽  
Smooth Muscle Actin ◽  
Collision Tumor ◽  
Stromal Tumor ◽  
Lower Body ◽  
Muscle Actin ◽  
First Case ◽  
S 100

AbstractReports of gastric collision tumors, comprising adenocarcinoma and gastrointestinal stromal tumor, are extremely rare. Here, we report the case of a 68-year-old male who was diagnosed with a lower-body, moderately differentiated, tubular-type adenocarcinoma and submucosal tumor and underwent an elective D2 distal gastrectomy. The tumor cells of the gastrointestinal stromal tumor were positive for H-caldesmon and CD117, weakly positive for smooth muscle actin and DOG-1, and negative for desmin, S-100 protein, CD31, and AE1/AE3. The tumor had grown into a mixed form of adenocarcinoma and gastrointestinal stromal tumor. Thus, we report the first case of a preoperatively diagnosed collision tumor in the stomach consisting of adenocarcinoma and gastrointestinal stromal tumor.

Mammary-type Myofibroblastoma with Leiomyomatous Differentiation: A Rare Variant with Potential Pitfalls

2021 ◽  
pp. 106689692110313
Author(s):  
Alexander M. Strait ◽  
Julia A. Bridge ◽  
Anthony J. Iafrate ◽  
Marilyn M. Li ◽  
Feng Xu ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Transcriptome Sequencing ◽  
Smooth Muscle Actin ◽  
The Body ◽  
Muscle Actin ◽  
Mature Adipose Tissue ◽  
Spindle Cells ◽  
Whole Transcriptome Sequencing ◽  
Whole Transcriptome

Myofibroblastoma is a rare, benign stromal tumor with a diverse morphologic spectrum. Mammary-type myofibroblastoma (MTMF) is the extra-mammary counterpart of this neoplasm and its occurrence throughout the body has become increasingly recognized. Similar morphologic variations of MTMF have now been described which mirror those seen in the breast. We describe a case of intra-abdominal MTMF composed of short fascicles of eosinophilic spindle cells admixed with mature adipose tissue. The spindle cells stained diffusely positive for CD34, desmin, smooth muscle actin, and h-caldesmon by immunohistochemistry. Concurrent loss of RB1 (13q14) and 13q34 loci were confirmed by fluorescence in situ hybridization whereas anchored multiplex PCR and whole transcriptome sequencing did not reveal any pathognomonic fusions suggesting an alternative diagnosis. To the best of our knowledge this is the first documented case of leiomyomatous variant of MTMF.

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