Long-Lasting Effects of GSPE on Ileal GLP-1R Gene Expression Are Associated with a Hypomethylation of the GLP-1R Promoter in Female Wistar Rats

Flavonoids have been shown to modulate GLP-1 in obesity. GLP-1 induces some of its effects through the intestinal GLP-1 receptor (GLP-1R), though no data exist on how flavonoids affect this receptor. Here, we examine how a dose of grape seed proanthocyanidin extract (GSPE) with anti-obesity activity affects intestinal GLP-1R and analyze whether epigenetics play a role in the long-lasting effects of GSPE. We found that 10-day GSPE administration prior to the cafeteria diet upregulated GLP-1R mRNA in the ileum 17 weeks after the GSPE treatment. This was associated with a hypomethylation of the GLP-1R promoter near the region where the SP1 transcription factor binds. In the colon, the cafeteria diet upregulated GLP-1R without showing any GSPE effect. In conclusion, we have identified long-lasting GSPE effects on GLP-1R gene expression in the ileum that are partly mediated by hypomethylation at the gene promoter and may affect the SP1 binding factor.

Download Full-text

A critical role of Sp1 transcription factor in regulating gene expression in response to insulin and other hormones

Life Sciences ◽

10.1016/j.lfs.2008.06.024 ◽

2008 ◽

Vol 83 (9-10) ◽

pp. 305-312 ◽

Cited By ~ 79

Author(s):

Solomon S. Solomon ◽

Gipsy Majumdar ◽

Antonio Martinez-Hernandez ◽

Rajendra Raghow

Keyword(s):

Gene Expression ◽

Transcription Factor ◽

Critical Role ◽

Sp1 Transcription Factor

Download Full-text

Estrogen regulation of LRP16 gene expression involves Sp1 transcription factor

Chinese Journal of Cancer Research ◽

10.1007/s11670-006-0251-2 ◽

2006 ◽

Vol 18 (4) ◽

pp. 251-256

Author(s):

Yi-ling Si ◽

Wei-dong Han ◽

Ya-li Zhao ◽

Qi Li ◽

Hao-jie Hao ◽

...

Keyword(s):

Gene Expression ◽

Transcription Factor ◽

Estrogen Regulation ◽

Sp1 Transcription Factor

Download Full-text

Mechanisms of mammalian zinc-regulated gene expression

Biochemical Society Transactions ◽

10.1042/bst0361262 ◽

2008 ◽

Vol 36 (6) ◽

pp. 1262-1266 ◽

Cited By ~ 38

Author(s):

Kelly A. Jackson ◽

Ruth A. Valentine ◽

Lisa J. Coneyworth ◽

John C. Mathers ◽

Dianne Ford

Keyword(s):

Gene Expression ◽

Transcription Factor ◽

Transcriptional Repression ◽

Mammalian Cells ◽

Gene Promoter ◽

Mrna Levels ◽

Promoter Regions ◽

Mrna Stabilization ◽

Transcriptional Regulatory ◽

Regulated Gene Expression

Mechanisms through which gene expression is regulated by zinc are central to cellular zinc homoeostasis. In this context, evidence for the involvement of zinc dyshomoeostasis in the aetiology of diseases, including Type 2 diabetes, Alzheimer's disease and cancer, highlights the importance of zinc-regulated gene expression. Mechanisms elucidated in bacteria and yeast provide examples of different possible modes of zinc-sensitive gene regulation, involving the zinc-regulated binding of transcriptional activators and repressors to gene promoter regions. A mammalian transcriptional regulatory mechanism that mediates zinc-induced transcriptional up-regulation, involving the transcription factor MTF1 (metal-response element-binding transcription factor 1), has been studied extensively. Gene responses in the opposite direction (reduced mRNA levels in response to increased zinc availability) have been observed in mammalian cells, but a specific transcriptional regulatory process responsible for such a response has yet to be identified. Examples of single zinc-sensitive transcription factors regulating gene expression in opposite directions are emerging. Although zinc-induced transcriptional repression by MTF1 is a possible explanation in some specific instances, such a mechanism cannot account for repression by zinc of all mammalian genes that show this mode of regulation, indicating the existence of as yet uncharacterized mechanisms of zinc-regulated transcription in mammalian cells. In addition, recent findings reveal a role for effects of zinc on mRNA stability in the regulation of specific zinc transporters. Our studies on the regulation of the human gene SLC30A5 (solute carrier 30A5), which codes for the zinc transporter ZnT5, have revealed that this gene provides a model system by which to study both zinc-induced transcriptional down-regulation and zinc-regulated mRNA stabilization.

Download Full-text

Blocking Sp1 Transcription Factor Broadly Inhibits Extracellular Matrix Gene Expression In Vitro and In Vivo: Implications for the Treatment of Tissue Fibrosis

Journal of Investigative Dermatology ◽

10.1046/j.1523-1747.2001.01326.x ◽

2001 ◽

Vol 116 (5) ◽

pp. 755-763 ◽

Cited By ~ 92

Author(s):

Franck Verrecchia ◽

Alain Mauviel ◽

Jérôme Rossert

Keyword(s):

Gene Expression ◽

Extracellular Matrix ◽

Transcription Factor ◽

Tissue Fibrosis ◽

Sp1 Transcription Factor ◽

Matrix Gene

Download Full-text

Isolation and functional characterization of Spirulina D6D gene promoter: Role of a putative GntR transcription factor in transcriptional regulation of D6D gene expression

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2007.11.018 ◽

2008 ◽

Vol 365 (4) ◽

pp. 643-649 ◽

Cited By ~ 10

Author(s):

Sanjukta Subudhi ◽

Pavinee Kurdrid ◽

Apiradee Hongsthong ◽

Matura Sirijuntarut ◽

Supapon Cheevadhanarak ◽

...

Keyword(s):

Gene Expression ◽

Transcription Factor ◽

Transcriptional Regulation ◽

Functional Characterization ◽

Gene Promoter

Download Full-text

MGMT DNA repair gene promoter/enhancer haplotypes alter transcription factor binding and gene expression

Cellular Oncology ◽

10.1007/s13402-016-0286-4 ◽

2016 ◽

Vol 39 (5) ◽

pp. 435-447 ◽

Cited By ~ 6

Author(s):

Meixiang Xu ◽

Courtney E. Cross ◽

Jordan T. Speidel ◽

Sherif Z. Abdel-Rahman

Keyword(s):

Gene Expression ◽

Dna Repair ◽

Transcription Factor ◽

Gene Promoter ◽

Transcription Factor Binding ◽

Repair Gene ◽

Factor Binding ◽

Dna Repair Gene

Download Full-text

Sp1 transcription factor promotes TMEPAI gene expression and contributes to cell proliferation

Cell Proliferation ◽

10.1111/cpr.12292 ◽

2016 ◽

Vol 49 (6) ◽

pp. 710-719 ◽

Cited By ~ 11

Author(s):

Yuyin Li ◽

Ailong Guo ◽

Yajuan Feng ◽

Yueying Zhang ◽

Jianjun Wang ◽

...

Keyword(s):

Gene Expression ◽

Cell Proliferation ◽

Transcription Factor ◽

Sp1 Transcription Factor

Download Full-text

Effects of an Intermittent Grape-Seed Proanthocyanidin (GSPE) Treatment on a Cafeteria Diet Obesogenic Challenge in Rats

Nutrients ◽

10.3390/nu10030315 ◽

2018 ◽

Vol 10 (3) ◽

pp. 315 ◽

Cited By ~ 14

Author(s):

Iris Ginés ◽

Katherine Gil-Cardoso ◽

Joan Serrano ◽

Àngela Casanova-Martí ◽

MTeresa Blay ◽

...

Keyword(s):

Grape Seed ◽

Cafeteria Diet ◽

Grape Seed Proanthocyanidin

Download Full-text

Cell cycle-regulated transcription in fission yeast

Biochemical Society Transactions ◽

10.1042/bst0320967 ◽

2004 ◽

Vol 32 (6) ◽

pp. 967-972 ◽

Cited By ~ 11

Author(s):

C.J. McInerny

Keyword(s):

Gene Expression ◽

Cell Cycle ◽

Transcription Factor ◽

Fission Yeast ◽

Model Organism ◽

Alternative Form ◽

Regulation Of Transcription ◽

Transcription Control ◽

Binding Factor ◽

Transcription Factor Complex

A fundamental process in biology is the mechanism by which cells duplicate and divide to produce two identical daughter cells. The fission yeast, Schizosaccharomyces pombe, has proved to be an excellent model organism to study the role that gene expression plays in this process. The basic paradigm emerging is that a number of groups of genes are expressed in successive waves at different cell cycle times. Transcription of a particular group is controlled by a common DNA motif present in each gene's promoter, bound by a transcription factor complex. Each motif and transcription factor complex is specific to the time in the cell cycle when the group of genes is expressed. Examples of this are the MBF (MCB-binding factor)/MCB (MluI cell cycle box) system controlling gene expression at the start of S-phase, and PBF (PCB-binding factor)/PCB (Pombe cell cycle box) regulation of transcription at the end of mitosis. In some cases, these transcription control systems also operate during the alternative form of cell division, meiosis.

Download Full-text