scholarly journals P-N Junction-Based Si Biochips with Ring Electrodes for Novel Biosensing Applications

Biosensors ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 120 ◽  
Author(s):  
Mahdi Kiani ◽  
Nan Du ◽  
Manja Vogel ◽  
Johannes Raff ◽  
Uwe Hübner ◽  
...  
Keyword(s):  
Equivalent Circuit ◽  
Deionized Water ◽  
Bacterial Suspension ◽  
Electrode Structure ◽  
Two Phase ◽  
Electrical Equivalent Circuit ◽  
Lysinibacillus Sphaericus ◽  
Electrode Region ◽  
Impedance Characteristics ◽  
Number Of Bacteria

In this work, we report on the impedance of p-n junction-based Si biochips with gold ring top electrodes and unstructured platinum bottom electrodes which allows for counting target biomaterial in a liquid-filled ring top electrode region. The systematic experiments on p-n junction-based Si biochips fabricated by two different sets of implantation parameters (i.e. biochips PS5 and BS5) are studied, and the comparable significant change of impedance characteristics in the biochips in dependence on the number of bacteria suspension, i.e., Lysinibacillus sphaericus JG-A12, in Deionized water with an optical density at 600 nm from OD600 = 4–16 in the electrode ring region is demonstrated. Furthermore, with the help of the newly developed two-phase electrode structure, the modeled capacitance and resistance parameters of the electrical equivalent circuit describing the p-n junction-based biochips depend linearly on the number of bacteria in the ring top electrode region, which successfully proves the potential performance of p-n junction-based Si biochips in observing the bacterial suspension. The proposed p-n junction-based biochips reveal perspective applications in medicine and biology for diagnosis, monitoring, management, and treatment of diseases.

In Vitro morphological, optical and microbiological evaluation of nanosilver fluoride in the remineralization of deciduous teeth enamel

2018 ◽  
Vol 7 (6) ◽  
pp. 509-520 ◽  
Author(s):  
Amitis Vieira Costa e Silva ◽  
Joás Araújo Teixeira ◽  
Cláudia C.B.O. Mota ◽  
Emery Clayton Cabral Correia Lins ◽  
Paulo Correia de Melo Júnior ◽  
...  
Keyword(s):  
Dental Enamel ◽  
Deionized Water ◽  
Deciduous Teeth ◽  
Bacterial Suspension ◽  
Significant Difference ◽  
Ph Cycling ◽  
Optical Coherence Tomography Imaging ◽  
Caries Lesions ◽  
Mixed Linear Regression Model

AbstractBackgroundNanosilver fluoride (NSF) was developed as an alternative in the prevention of dental caries.PurposeThe aim of this study was to test the remineralizing action of NSF on incipient enamel caries and its antimicrobial action on the acid production and adhesion of Streptococcus mutans.MethodsDeciduous enamel fragments were treated with sodium fluoride (NaF), NSF and deionized water. Microhardness, fluorescence spectroscopy and optical coherence tomography imaging were performed on each specimen before chemical caries induction, after caries induction and after 14 days of pH cycling. The treated enamel fragments were also placed into test tubes containing bacterial suspension and saliva. The pH readings and quantification of the adhered microorganisms to the dental enamel were determined. Analysis of variance, Kruskal-Wallis, Mann-Whitney, Tukey and mixed linear regression model were applied.ResultsNSF and NaF were effective in enamel remineralization, with a statistically significant difference (p<0.001) to deionized water, and they had no statistically significant difference between themselves (p>0.005). NSF had greater effectiveness compared to NaF in preventing decreases of pH and adhesion of S. mutans to the enamel surface, with statistically significant (p<0.001) differences.ConclusionNSF may be more effective than conventional fluorides in treating incipient caries lesions due to its remineralization and antibacterial actions.

scholarly journals Root Colonization by Agrobacterium tumefaciens Is Reduced in cel, attB,attD, and attR Mutants

1998 ◽  
Vol 64 (7) ◽  
pp. 2341-2345 ◽  
Author(s):  
Ann G. Matthysse ◽  
Susan McMahan
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Root Length ◽  
Root Colonization ◽  
Bacterial Pathogenesis ◽  
Bacterial Suspension ◽  
Cellulose Synthesis ◽  
Wild Type ◽  
Tomato Roots ◽  
Extensive Growth ◽  
Number Of Bacteria

ABSTRACT Root colonization by Agrobacterium tumefaciens was measured by using tomato and Arabidopsis thaliana roots dipped in a bacterial suspension and planted in soil. Wild-type bacteria showed extensive growth on tomato roots; the number of bacteria increased from 103 bacteria/cm of root length at the time of inoculation to more than 107 bacteria/cm after 10 days. The numbers of cellulose-minus and nonattachingattB, attD, and attR mutant bacteria were less than 1/10,000th the number of wild-type bacteria recovered from tomato roots. On roots of A. thalianaecotype Landsberg erecta, the numbers of wild-type bacteria increased from about 30 to 8,000 bacteria/cm of root length after 8 days. The numbers of cellulose-minus and nonattaching mutant bacteria were 1/100th to 1/10th the number of wild-type bacteria recovered after 8 days. The attachment of A. tumefaciens to cut A. thaliana roots incubated in 0.4% sucrose and observed with a light microscope was also reduced with cel andatt mutants. These results suggest that cellulose synthesis and attachment genes play a role in the ability of the bacteria to colonize roots, as well as in bacterial pathogenesis.

Engineering Aspects and Parameter Evaluation of Ozone Generator

2021 ◽  
Vol 12 ◽  
pp. 19-23
Author(s):  
Mohamed B. El_Mashede ◽  
Magdy M. Zaky ◽  
A. A. Saleh ◽  
M. EL_Hanash
Keyword(s):  
Equivalent Circuit ◽  
Nonlinear Model ◽  
Gas Flow ◽  
Cooling Water ◽  
Nuclear Research ◽  
Main Element ◽  
Ozone Generator ◽  
Secondary System ◽  
Electrical Equivalent Circuit ◽  
Parameter Evaluation

This paper introduces the Ozone generator parameters analysis and its engineering design aspects. Practically, Ozone is successfully used in water treatment applications; furthermore, it can be used to treat the secondary system cooling water in nuclear research reactors. The main element in this process is the Ozone generator; it has many parameters that need to be set at its design. These parameters can be divided into those related to the electrical equivalent circuit of corona dielectric barrier discharge (DBD), those associated with injected gas flow between the two electrodes, and those belonging to temperature of the electrodes. This paper is intended to those parameters that are related to the electrical equivalent circuit which has two standard models: linear and nonlinear. In this regard, the determination of the component values of nonlinear model can be achieved very hardly. To handle such determination, the nonlinear model can be approximately treated as a linear model in an approaching fashion. Based on this approximation, Lissajous plot and differential evolution (DE) methodologies are used for the computation of DBD ozone chamber parameters. Additionally, a new theoretical technique will be presented

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