Voltage-Sensitive Dye versus Intrinsic Signal Optical Imaging: Comparison of Tactile Responses in Primary and Secondary Somatosensory Cortices of Rats

Studies using functional magnetic resonance imaging assume that hemodynamic responses have roughly linear relationships with underlying neural activity. However, to accurately investigate the neurovascular transfer function and compare its variability across brain regions, it is necessary to obtain full-field imaging of both electrophysiological and hemodynamic responses under various stimulus conditions with superior spatiotemporal resolution. Optical imaging combined with voltage-sensitive dye (VSD) and intrinsic signals (IS) is a powerful tool to address this issue. We performed VSD and IS imaging in the primary (S1) and secondary (S2) somatosensory cortices of rats to obtain optical maps of whisker-evoked responses. There were characteristic differences in sensory responses between the S1 and S2 cortices: VSD imaging revealed more localized excitatory and stronger inhibitory neural activity in S1 than in S2. IS imaging revealed stronger metabolic responses in S1 than in S2. We calculated the degree of response to compare the sensory responses between cortical regions and found that the ratio of the degree of response of S2 to S1 was similar, irrespective of whether the ratio was determined by VSD or IS imaging. These results suggest that neurovascular coupling does not vary between the S1 and S2 cortices.

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Voltage-sensitive dye versus intrinsic signal optical imaging: comparison of optically determined functional maps from rat barrel cortex

Neuroreport ◽

10.1097/00001756-200109170-00027 ◽

2001 ◽

Vol 12 (13) ◽

pp. 2889-2894 ◽

Cited By ~ 39

Author(s):

Ichiro Takashima ◽

Riichi Kajiwara ◽

Toshio Iijima

Keyword(s):

Optical Imaging ◽

Barrel Cortex ◽

Voltage Sensitive Dye ◽

Intrinsic Signal ◽

Intrinsic Signal Optical Imaging

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An Implantable Cranial Window Using a Collagen Membrane for Chronic Voltage-Sensitive Dye Imaging

Micromachines ◽

10.3390/mi10110789 ◽

2019 ◽

Vol 10 (11) ◽

pp. 789 ◽

Cited By ~ 1

Author(s):

Nobuo Kunori ◽

Ichiro Takashima

Keyword(s):

Neural Activity ◽

Network Activity ◽

Optical Methods ◽

Collagen Membrane ◽

Spatiotemporal Resolution ◽

Voltage Sensitive Dye ◽

Cranial Window ◽

Major Disadvantage ◽

Optically Transparent ◽

Dye Staining

Incorporating optical methods into implantable neural sensing devices is a challenging approach for brain–machine interfacing. Specifically, voltage-sensitive dye (VSD) imaging is a powerful tool enabling visualization of the network activity of thousands of neurons at high spatiotemporal resolution. However, VSD imaging usually requires removal of the dura mater for dye staining, and thereafter the exposed cortex needs to be protected using an optically transparent artificial dura. This is a major disadvantage that limits repeated VSD imaging over the long term. To address this issue, we propose to use an atelocollagen membrane as the dura substitute. We fabricated a small cranial chamber device, which is a tubular structure equipped with a collagen membrane at one end of the tube. We implanted the device into rats and monitored neural activity in the frontal cortex 1 week following surgery. The results indicate that the collagen membrane was chemically transparent, allowing VSD staining across the membrane material. The membrane was also optically transparent enough to pass light; forelimb-evoked neural activity was successfully visualized through the artificial dura. Because of its ideal chemical and optical manipulation capability, this collagen membrane may be widely applicable in various implantable neural sensors.

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Voltage-Sensitive Dye and Intrinsic Signal Optical Imaging

Neurophotonics and Brain Mapping ◽

10.1201/9781315373058-7 ◽

2017 ◽

pp. 101-116

Author(s):

Vassiliy Tsytsarev ◽

Reha S. Erzurumlu

Keyword(s):

Optical Imaging ◽

Voltage Sensitive Dye ◽

Intrinsic Signal ◽

Intrinsic Signal Optical Imaging

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Optical imaging of neural activity of auditory cortex in response to sounds containing a silent period

Neuroscience Research ◽

10.1016/s0168-0102(00)81786-1 ◽

2000 ◽

Vol 38 ◽

pp. S156

Author(s):

J Horikawa

Keyword(s):

Auditory Cortex ◽

Optical Imaging ◽

Neural Activity ◽

Silent Period

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Neural substrates of propranolol-induced impairments in the reconsolidation of nicotine-associated memories in smokers

Translational Psychiatry ◽

10.1038/s41398-021-01566-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Xiao Lin ◽

Jiahui Deng ◽

Kai Yuan ◽

Qiandong Wang ◽

Lin Liu ◽

...

Keyword(s):

Neural Activity ◽

Neural Substrates ◽

Brain Regions ◽

Reward Processing ◽

Memory Reconsolidation ◽

Functional Magnetic Resonance ◽

Resonance Imaging ◽

Postcentral Gyrus ◽

Propranolol Group ◽

Propranolol Administration

AbstractThe majority of smokers relapse even after successfully quitting because of the craving to smoking after unexpectedly re-exposed to smoking-related cues. This conditioned craving is mediated by reward memories that are frequently experienced and stubbornly resistant to treatment. Reconsolidation theory posits that well-consolidated memories are destabilized after retrieval, and this process renders memories labile and vulnerable to amnestic intervention. This study tests the retrieval reconsolidation procedure to decrease nicotine craving among people who smoke. In this study, 52 male smokers received a single dose of propranolol (n = 27) or placebo (n = 25) before the reactivation of nicotine-associated memories to impair the reconsolidation process. Craving for smoking and neural activity in response to smoking-related cues served as primary outcomes. Functional magnetic resonance imaging was performed during the memory reconsolidation process. The disruption of reconsolidation by propranolol decreased craving for smoking. Reactivity of the postcentral gyrus in response to smoking-related cues also decreased in the propranolol group after the reconsolidation manipulation. Functional connectivity between the hippocampus and striatum was higher during memory reconsolidation in the propranolol group. Furthermore, the increase in coupling between the hippocampus and striatum positively correlated with the decrease in craving after the reconsolidation manipulation in the propranolol group. Propranolol administration before memory reactivation disrupted the reconsolidation of smoking-related memories in smokers by mediating brain regions that are involved in memory and reward processing. These findings demonstrate the noradrenergic regulation of memory reconsolidation in humans and suggest that adjunct propranolol administration can facilitate the treatment of nicotine dependence. The present study was pre-registered at ClinicalTrials.gov (registration no. ChiCTR1900024412).

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