scholarly journals A Bioorthogonally Synthesized and Disulfide-Containing Fluorescence Turn-On Chemical Probe for Measurements of Butyrylcholinesterase Activity and Inhibition in the Presence of Physiological Glutathione

Catalysts ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1169
Author(s):  
Ming-Mao Gong ◽  
Chia-Yen Dai ◽  
Scott Severance ◽  
Chi-Ching Hwang ◽  
Bo-Kai Fang ◽  
...  
Keyword(s):  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Kinetic Studies ◽  
Chemical Probe ◽  
Turn On ◽  
Bche Activity ◽  
Substitution Mechanism ◽  
Butyrylcholinesterase Activity ◽  
Fluorescence Turn On ◽  
Derivatives Of

Butyrylcholinesterase (BChE) is a biomarker in human blood. Aberrant BChE activity has been associated with human diseases. Here we developed a fluorescence resonance energy transfer (FRET) chemical probe to specifically quantify BChE activity in serum, while simultaneously discriminating against glutathione (GSH). The FRET chemical probe 11 was synthesized from a key trifunctional bicyclononyne exo-6 and derivatives of 5-(2-aminoethylamino)-1-naphthalenesulfonic acid (EDANS) and 4-[4-(dimethylamino)phenylazo]benzoic acid (DABCYL). EDANS fluorescence visualization and kinetic analysis of 11 in the presence of diverse compounds confirmed the outstanding reactivity and specificity of 11 with thiols. The thiol-dependent fluorescence turn-on property of 11 was attributed to a general base-catalyzed SN2 nucleophilic substitution mechanism and independent of metal ions. Moreover, all thiols, except GSH, reacted swiftly with 11. Kinetic studies of 11 in the presence of covalently modified GSH derivatives corroborated that the steric hindrance of 11 imposing on GSH was the likely cause of the distinguished reactivity. Since GSH commonly interferes in assays measuring BChE activity in blood samples, the 11-based fluorescent assay was employed to directly quantify BChE activity without GSH interference, and delivered a linear range of 4.3–182.2 U L−1 for BChE activity with detection limit of 4.3 U L−1, and accurately quantified serum BChE activity in the presence of 10 μM GSH. Finally, the 11-based assay was exploited to determine Ki of 5 nM for tacrine inhibition on BChE catalysis. We are harnessing the modulated characteristics of 6 to synthesize advanced chemical probes able to more sensitively screen for BChE inhibitors and quantify BChE activity in serum.

Amplified Fluorescence Turn-on Assay for Mercury(II) Based on Conjugated Polyfluorene Derivatives and Nanospheres

2008 ◽  
Vol 1134 ◽  
Author(s):  
Yusong Wang ◽  
Bin Liu
Keyword(s):  
Metal Ion ◽  
Resonance Energy Transfer ◽  
Fluorescence Emission ◽  
Resonance Energy ◽  
High Sensitivity ◽  
Probe Design ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
Fluorescence Turn On ◽  
Polyfluorene Derivatives

AbstractDetection of mercury with high sensitivity and selectivity constitutes a significant research concern. Here, we report an amplified fluorescence turn-on assay for mercury(II) with an improved performance. This sensing system takes advantage of optically amplifying fluorescent conjugated polyfluorene derivatives and DNA immobilized silica nanospheres (NSs) in addition to the specific thymine- mercury(II)-thymine(T- Hg2+-T) interaction. The employment of ion-specific T- Hg2+-T coordination increases the melting temperature (Tm) of the double-stranded DNA (dsDNA) on the hybridized NS surface. After thermal washing at 45 °C, the Hg2+ treated sample (dsDNA-NS) was effectively differentiated from that treated with nonspecific ions through monitoring fluorescence emission of fluorescein (Fl) labeled target DNA remained on the NS surface. Finally, a cationic conjugated polyfluorene derivative (CCP) was introduced to electrostatically associate with the DNA molecules on the NS surface, resulting in an amplified Fl signal via fluorescence resonance energy transfer (FRET) from the CCP to the dye molecule. In comparison with the use of Fl alone as a signal reporter, the presence of CCP significantly enhances the detection fluorescence intensity, reduces false-positive signal, and improves the detection selectivity for mercury(II). Further improvement in the probe design could yield more efficient metal ion sensors, which have the potential to be operated at room temperature and for the detection of other metal ions besides mercury(II).

Electrochemiluminescence resonance energy transfer between methylene blue and Ru(bpy)32+-doped silica nanoparticles and its application in the “turn-on” detection of glucose

2019 ◽  
Vol 43 (23) ◽  
pp. 9226-9231
Author(s):  
Yuling Fu ◽  
Wenjing Qi ◽  
Hongkun He ◽  
Maoyu Zhao ◽  
Di Wu ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Energy Transfer ◽  
Silica Nanoparticles ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Turn On

The ECL donor Ru(bpy)32+-doped silica nanoparticles and acceptor methylene blue were applied in the electrochemiluminescence resonance energy transfer-based detection of glucose.

Sensitive turn-on fluorescent detection of melamine based on fluorescence resonance energy transfer

The Analyst ◽  
2011 ◽  
Vol 136 (8) ◽  
pp. 1659 ◽  
Author(s):  
Liangqia Guo ◽  
Jianhai Zhong ◽  
Jinmei Wu ◽  
FengFu Fu ◽  
Guonan Chen ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescent Detection ◽  
Turn On ◽  
Fluorescence Resonance

Electrochemiluminescence Resonance Energy Transfer Based on Ru(phen)32+-Doped Silica Nanoparticles and Its Application in “Turn-on” Detection of Ozone

2013 ◽  
Vol 85 (6) ◽  
pp. 3207-3212 ◽  
Author(s):  
Wenjing Qi ◽  
Di Wu ◽  
Jianming Zhao ◽  
Zhongyuan Liu ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Silica Nanoparticles ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Turn On
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