scholarly journals Metabolomics-Guided Analysis of the Biocatalytic Conversion of Sclareol to Ambradiol by Hyphozyma roseoniger

Catalysts ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 55
Author(s):  
Efficient N. Ncube ◽  
Paul A. Steenkamp ◽  
Chris W. van der Westhuyzen ◽  
Lucia H. Steenkamp ◽  
Ian A. Dubery
Keyword(s):  
Growth Stage ◽  
Integrated Approach ◽  
Valuable Component ◽  
Dynamic Changes ◽  
Whole Cell ◽  
New Findings ◽  
Whole Cell Biocatalyst ◽  
The Matrix ◽  
Dimorphic Yeast ◽  
Whole Cell Biotransformation

The biocatalytic conversion of sclareol to ambradiol, a valuable component in the fragrance industry, using whole-cell biotransformation by the dimorphic yeast Hyphozyma roseoniger, was investigated using metabolomics tools. An integrated approach was used to identify and quantify the participating intermediates in this bioconversion using both nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography coupled to mass spectrometry (LC–MS). This study entailed growth stage-dependent analysis of H. roseoniger suspensions grown in batch culture over a 14-day period, beginning with a three-day induction period using 20 mg/200 mL sclareol, followed by a further 1 g/200 mL sclareol dose to enable ambradiol production. The progress of the bioconversion and the resulting dynamic changes to the metabolome were monitored using NMR analysis and semi-targeted LC–MS metabolomics. This outlined the molecular conversions occurring within the matrix and no novel intermediates participating in the sclareol to ambradiol conversion could be identified. This study presents new findings about the transformative capabilities of H. roseoniger as a whole cell biocatalyst, highlighting its potential utility in similar applications.

scholarly journals Tuning a bi-enzymatic cascade reaction in Escherichia coli to facilitate NADPH regeneration for ε-caprolactone production

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jinghui Xiong ◽  
Hefeng Chen ◽  
Ran Liu ◽  
Hao Yu ◽  
Min Zhuo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Binding Sites ◽  
Regeneration System ◽  
Nadph Regeneration ◽  
Whole Cell ◽  
Cyclohexanone Monooxygenase ◽  
Ribosome Binding ◽  
Ribosome Binding Sites ◽  
Whole Cell Biocatalyst ◽  
Substrate Tolerance

Abstractε-Caprolactone is a monomer of poly(ε-caprolactone) which has been widely used in tissue engineering due to its biodegradability and biocompatibility. To meet the massive demand for this monomer, an efficient whole-cell biocatalytic approach was constructed to boost the ε-caprolactone production using cyclohexanol as substrate. Combining an alcohol dehydrogenase (ADH) with a cyclohexanone monooxygenase (CHMO) in Escherichia coli, a self-sufficient NADPH-cofactor regeneration system was obtained. Furthermore, some improved variants with the better substrate tolerance and higher catalytic ability to ε-caprolactone production were designed by regulating the ribosome binding sites. The best mutant strain exhibited an ε-caprolactone yield of 0.80 mol/mol using 60 mM cyclohexanol as substrate, while the starting strain only got a conversion of 0.38 mol/mol when 20 mM cyclohexanol was supplemented. The engineered whole-cell biocatalyst was used in four sequential batches to achieve a production of 126 mM ε-caprolactone with a high molar yield of 0.78 mol/mol.

Antityrosinase Mechanism and Antimelanogenic Effect of Arbutin Esters Synthesis Catalyzed by Whole-Cell Biocatalyst

2021 ◽  
Vol 69 (14) ◽  
pp. 4243-4252
Author(s):  
Haixia Xu ◽  
Xiaofeng Li ◽  
Xuan Xin ◽  
Lan Mo ◽  
Yucong Zou ◽  
...  
Keyword(s):  
Whole Cell ◽  
Whole Cell Biocatalyst

Asymmetric reduction of a key intermediate of eslicarbazepine acetate using whole cell biotransformation in a biphasic medium

2012 ◽  
Vol 2 (8) ◽  
pp. 1602 ◽  
Author(s):  
Manpreet Singh ◽  
Sawraj Singh ◽  
Sateesh Deshaboina ◽  
Hare Krishnen ◽  
Richard Lloyd ◽  
...  
Keyword(s):  
Asymmetric Reduction ◽  
Eslicarbazepine Acetate ◽  
Whole Cell ◽  
Whole Cell Biotransformation ◽  
Biphasic Medium

scholarly journals Bacteriophage-Based Biosensing of Pseudomonas aeruginosa: An Integrated Approach for the Putative Real-Time Detection of Multi-Drug-Resistant Strains

Biosensors ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 124
Author(s):  
Liliam K. Harada ◽  
Waldemar Bonventi Júnior ◽  
Erica C. Silva ◽  
Thais J. Oliveira ◽  
Fernanda C. Moreli ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacterial Infections ◽  
Research Effort ◽  
Acquired Resistance ◽  
Integrated Approach ◽  
Healthcare Services ◽  
Host Cells ◽  
Color Changes ◽  
Metabolic Versatility ◽  
The Matrix

During the last decennium, it has become widely accepted that ubiquitous bacterial viruses, or bacteriophages, exert enormous influences on our planet’s biosphere, killing between 4–50% of the daily produced bacteria and constituting the largest genetic diversity pool on our planet. Currently, bacterial infections linked to healthcare services are widespread, which, when associated with the increasing surge of antibiotic-resistant microorganisms, play a major role in patient morbidity and mortality. In this scenario, Pseudomonas aeruginosa alone is responsible for ca. 13–15% of all hospital-acquired infections. The pathogen P. aeruginosa is an opportunistic one, being endowed with metabolic versatility and high (both intrinsic and acquired) resistance to antibiotics. Bacteriophages (or phages) have been recognized as a tool with high potential for the detection of bacterial infections since these metabolically inert entities specifically attach to, and lyse, bacterial host cells, thus, allowing confirmation of the presence of viable cells. In the research effort described herein, three different phages with broad lytic spectrum capable of infecting P. aeruginosa were isolated from environmental sources. The isolated phages were elected on the basis of their ability to form clear and distinctive plaques, which is a hallmark characteristic of virulent phages. Next, their structural and functional stabilization was achieved via entrapment within the matrix of porous alginate, biopolymeric, and bio-reactive, chromogenic hydrogels aiming at their use as sensitive matrices producing both color changes and/or light emissions evolving from a reaction with (released) cytoplasmic moieties, as a bio-detection kit for P. aeruginosa cells. Full physicochemical and biological characterization of the isolated bacteriophages was the subject of a previous research paper.

Engineered whole-cell biocatalyst-based detoxification and detection of neurotoxic organophosphate compounds

2014 ◽  
Vol 32 (3) ◽  
pp. 652-662 ◽  
Author(s):  
Chang Sup Kim ◽  
Jeong Hyun Seo ◽  
Dong Gyun Kang ◽  
Hyung Joon Cha
Keyword(s):  
Whole Cell ◽  
Organophosphate Compounds ◽  
Whole Cell Biocatalyst

Production of biodiesel by immobilized whole-cell biocatalyst

2008 ◽  
Vol 136 ◽  
pp. S385-S386
Author(s):  
Jyh-Ping Chen ◽  
Shu-Chin Chang
Keyword(s):  
Whole Cell ◽  
Whole Cell Biocatalyst

Glyoxylate Carboligase-based Whole-Cell Biotransformation of Formaldehyde into Ethylene Glycol via Glycolaldehyde

2021 ◽  
Author(s):  
Hye-Jin Jo ◽  
Jun-Hong Kim ◽  
Ye-Na Kim ◽  
Pil-Won Seo ◽  
Chae-Yun Kim ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Whole Cell ◽  
Whole Cell Biotransformation

A novel biocatalytic system for the synthesis of the industrially relevant C2 chemicals (e.g., ethylene glycol (3)) from formaldehyde (1) was established. The biocatalytic system consisted of a newly discovered...

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