General Patterns and Species-Specific Differences in the Organization of the Tubulin Cytoskeleton in Indeterminate Nodules of Three Legumes

The tubulin cytoskeleton plays an important role in establishing legume–rhizobial symbiosis at all stages of its development. Previously, tubulin cytoskeleton organization was studied in detail in the indeterminate nodules of two legume species, Pisum sativum and Medicago truncatula. General as well as species-specific patterns were revealed. To further the understanding of the formation of general and species-specific microtubule patterns in indeterminate nodules, the tubulin cytoskeleton organization was studied in three legume species (Vicia sativa, Galega orientalis, and Cicer arietinum). It is shown that these species differ in the shape and size of rhizobial cells (bacteroids). Immunolocalization of microtubules revealed the universality of cortical and endoplasmic microtubule organization in the meristematic cells, infected cells of the infection zone, and uninfected cells in nodules of the three species. However, there are differences in the endoplasmic microtubule organization in nitrogen-fixing cells among the species, as confirmed by quantitative analysis. It appears that the differences are linked to bacteroid morphology (both shape and size).

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Flight-call as species-specific signal in South American parrots and the effect of species relatedness in call similarity

Revista Brasileira de Ornitologia ◽

10.1007/bf03544392 ◽

2017 ◽

Vol 25 (3) ◽

pp. 143-151 ◽

Cited By ~ 1

Author(s):

Carlos B. de Araújo ◽

Paulo A. M. Marques ◽

Jacques M. E. Vielliard

Keyword(s):

South American ◽

Specific Signal ◽

As Species ◽

Species Specific

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Detection of Plasmodium falciparum DNA using repetitive DNA clones as species specific probes

Molecular and Biochemical Parasitology ◽

10.1016/0166-6851(87)90044-2 ◽

1987 ◽

Vol 22 (2-3) ◽

pp. 145-151 ◽

Cited By ~ 28

Author(s):

J. Werner Zolg ◽

L.Eugenio Andrade ◽

Ethel D. Scott

Keyword(s):

Plasmodium Falciparum ◽

Repetitive Dna ◽

As Species ◽

Species Specific

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Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia

Toxins ◽

10.3390/toxins10110460 ◽

2018 ◽

Vol 10 (11) ◽

pp. 460 ◽

Cited By ~ 1

Author(s):

Vesna Krnjaja ◽

Slavica Stanković ◽

Ana Obradović ◽

Tanja Petrović ◽

Violeta Mandić ◽

...

Keyword(s):

Fusarium Graminearum ◽

Gene Clusters ◽

Sensu Stricto ◽

Molecular Techniques ◽

Weather Data ◽

Morphological Observation ◽

Specific Primer ◽

Head Blight ◽

As Species ◽

Species Specific

Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 µg kg−1) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.

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Uneven distribution of cryptic diversity among higher taxa of parasitic worms

Biology Letters ◽

10.1098/rsbl.2010.0640 ◽

2010 ◽

Vol 7 (2) ◽

pp. 241-244 ◽

Cited By ~ 56

Author(s):

Robert Poulin

Keyword(s):

Cryptic Species ◽

Zoonotic Diseases ◽

Helminth Parasites ◽

Morphological Markers ◽

Molecular Tools ◽

Invasive Pathogens ◽

Higher Taxa ◽

As Species ◽

Species Specific ◽

Parasitic Worms

Cryptic species cause problems for estimates of biodiversity. In the case of parasites, cryptic species also plague efforts to detect potential zoonotic diseases or invasive pathogens. It is crucial to determine whether the likelihood of finding cryptic species differs among higher parasite taxa, to better calibrate estimates of diversity and monitor diseases. Using published reports of cryptic species of helminth parasites identified using molecular tools, I show that the number of species found is strongly related to the number of parasite individuals sequenced, weakly influenced by the number of host species from which parasites were obtained, and unaffected by the genetic markers used. After correction for these factors, more cryptic species of trematodes are found than in other helminth taxa. Although several features distinguish trematodes from other helminths, it is probable that our inability to discriminate among sibling species of trematodes results from their lack of structures serving as species-specific morphological markers. The available data suggest that current estimates of helminth diversity may need to be doubled (tripled for trematodes) to better reflect extant diversity.

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Restoration Scaling Approaches to Addressing Ecological Injury: The Habitat-Based Resource Equivalency Method

Environmental Management ◽

10.1007/s00267-019-01245-9 ◽

2020 ◽

Vol 65 (2) ◽

pp. 161-177 ◽

Cited By ~ 3

Author(s):

Mary Baker ◽

Adam Domanski ◽

Terill Hollweg ◽

Jason Murray ◽

Diana Lane ◽

...

Keyword(s):

Natural Resource ◽

Oil Spills ◽

Error Rates ◽

Hazardous Substances ◽

Habitat Equivalency Analysis ◽

Natural Resource Damage Assessment ◽

As Species ◽

Ecological Habitat ◽

Multiple Species ◽

Species Specific

AbstractNatural resource trustee agencies must determine how much, and what type of environmental restoration will compensate for injuries to natural resources that result from releases of hazardous substances or oil spills. To fulfill this need, trustees, and other natural resource damage assessment (NRDA) practitioners have relied on a variety of approaches, including habitat equivalency analysis (HEA) and resource equivalency analysis (REA). The purpose of this paper is to introduce the Habitat-Based Resource Equivalency Method (HaBREM), which integrates REA’s reproducible injury metrics and population modeling with HEA’s comprehensive habitat approach to restoration. HaBREM is intended to evaluate injury and restoration using organisms that use the habitat to represent ecological habitat functions. This paper seeks to expand and refine the use of organism-based metrics (biomass-based REA), providing an opportunity to integrate sublethal injuries to multiple species, as well as the potential to include error rates for injury and restoration parameters. Applied by NRDA practitioners in the appropriate context, this methodology can establish the relationship between benefits of compensatory restoration projects and injuries to plant or animal species within an affected habitat. HaBREM may be most effective where there are appropriate data supporting the linkage between habitat and species gains (particularly regionally specific habitat information), as well as species-specific monitoring data and predictions on the growth, density, productivity (i.e., rate of generation of biomass or individuals), and age distributions of indicator species.

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5S Ribosomal RNA Gene Spacers as Species-Specific Probes for Eight Species ofPythium

Phytopathology ◽

10.1094/phyto-86-581 ◽

1996 ◽

Vol 86 (6) ◽

pp. 581 ◽

Cited By ~ 38

Author(s):

Glen Klassen

Keyword(s):

Ribosomal Rna ◽

Ribosomal Rna Gene ◽

5S Ribosomal Rna ◽

As Species ◽

Species Specific

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PORCINE ENDOTHELIUM SUPPORTS TRANSENDOTHELIAL MIGRATION OF HUMAN LEUKOCYTE SUBPOPULATIONS: ANTI-PORCINE VASCULAR CELL ADHESION MOLECULE ANTIBODIES AS SPECIES-SPECIFIC BLOCKERS OF TRANSENDOTHELIAL MONOCYTE AND NATURAL KILLER CELL MIGRATION1

Transplantation Journal ◽

10.1097/00007890-200005150-00018 ◽

2000 ◽

Vol 69 (9) ◽

pp. 1837-1849 ◽

Cited By ~ 30

Author(s):

Elenor Hauzenberger ◽

Dan Hauzenberger ◽

Kjell Hultenby ◽

Jan Holgersson

Keyword(s):

Cell Adhesion ◽

Natural Killer Cell ◽

Adhesion Molecule ◽

Cell Adhesion Molecule ◽

Killer Cell ◽

Human Leukocyte ◽

Vascular Cell Adhesion ◽

Vascular Cell ◽

As Species ◽

Species Specific

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Selected mitochondrial genes as species markers of the Arctic Contracaecum osculatum complex

Journal of Helminthology ◽

10.1017/s0022149x11000332 ◽

2011 ◽

Vol 86 (2) ◽

pp. 252-258 ◽

Cited By ~ 5

Author(s):

J. Dzido ◽

A. Kijewska ◽

J. Rokicki

Keyword(s):

Barents Sea ◽

Intraspecific Variability ◽

Mitochondrial Genes ◽

Intraspecific Diversity ◽

The Arctic ◽

Protein Coding ◽

As Species ◽

Contracaecum Osculatum ◽

Species Markers ◽

Species Specific

AbstractThis study, aimed at testing the hypothesis that some mitochondrial genes can serve as species-specific markers, involved a comparison of the sequence variance of selected mitochondrial DNA genes of the Arctic Contracaecum osculatum species (C. osculatum A, C. osculatum B and C. osculatum C). We compared differences between five complete (ND2, CYTB, ND3, ND4L and ND6) and three partial (CO1, CO3 and ND5) protein-coding genes. The total length of the sequence of each of the 13 specimens was 4830 bp. The sample consisted of C. osculatum L3 larvae collected from Reinhardtius hippoglossoides and Gadus ogac from the Barents Sea and Davis Strait. The K2P distance values between the species ranged within 0.06–0.12, the intraspecific variability (0.01–0.03) proving 3–6 times lower. The lowest interspecific divergence was observed between C. osculatum A and C. osculatum B, whereas the highest intraspecific diversity was typical of C. osculatum C. Among the C. osculatum species studied, the highest nucleotide diversity was recorded in the CYTB, CO3 and ND5 genes. These genes may be useful in species identification of the very closely related Contracaecum sibling species.

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Ste20-like protein kinase SLK (LOSK) regulates microtubule organization by targeting dynactin to the centrosome

Molecular Biology of the Cell ◽

10.1091/mbc.e13-03-0137 ◽

2013 ◽

Vol 24 (20) ◽

pp. 3205-3214 ◽

Cited By ~ 14

Author(s):

Olga N. Zhapparova ◽

Artem I. Fokin ◽

Nadezhda E. Vorobyeva ◽

Sofia A. Bryantseva ◽

Elena S. Nadezhdina

Keyword(s):

Phosphorylation Site ◽

Cell Polarization ◽

Microtubule Organization ◽

Microtubule Binding ◽

Major Function ◽

Cytoskeleton Organization ◽

Dynein Motor ◽

Microtubule Binding Domain ◽

A Minor ◽

Radial Organization

The microtubule- and centrosome-associated Ste20-like kinase (SLK; long Ste20-like kinase [LOSK]) regulates cytoskeleton organization and cell polarization and spreading. Its inhibition causes microtubule disorganization and release of centrosomal dynactin. The major function of dynactin is minus end–directed transport along microtubules in a complex with dynein motor. In addition, dynactin is required for maintenance of the microtubule radial array in interphase cells, and depletion of its centrosomal pool entails microtubule disorganization. Here we demonstrate that SLK (LOSK) phosphorylates the p150Gluedsubunit of dynactin and thus targets it to the centrosome, where it maintains microtubule radial organization. We show that phosphorylation is required only for centrosomal localization of p150Gluedand does not affect its microtubule-organizing properties: artificial targeting of nonphosphorylatable p150Gluedto the centrosome restores microtubule radial array in cells with inhibited SLK (LOSK). The phosphorylation site is located in a microtubule-binding region that is variable for two isoforms (1A and 1B) of p150Gluedexpressed in cultured fibroblast-like cells (isoform 1B lacks 20 amino acids in the basic microtubule-binding domain). The fact that SLK (LOSK) phosphorylates only a minor isoform 1A of p150Gluedsuggests that transport and microtubule-organizing functions of dynactin are distinctly divided between the two isoforms. We also show that dynactin phosphorylation is involved in Golgi reorientation in polarized cells.

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