Preferential Begging Responses of Shiny Cowbirds to the Conspecific Chatter Call

Avian brood parasites lay their eggs into the nests of other species, which incubate them and raise the chicks until their independence. Despite living their early weeks of life surrounded by heterospecifics, young brood parasites have the ability to recognize and associate to conspecifics after independence. It has been proposed that the initial conspecific recognition develops when a young parasite encounters a unique species-specific signal that triggers the learning of other aspects of the producer of the signal. For cowbirds (Molothrus spp.), this species-specific signal is hypothesized to be the chatter call. Young birds also could express auditory biases, which in some cases lead to discrimination in favor of conspecific songs. Therefore, the perceptual selectivity for chatters might be also present in nestlings. Our aim was to assess if nestlings of the shiny cowbird (M. bonariensis) present a preferential begging response to conspecific chatter calls. We evaluated if they respond more to the parasitic vocalization than host chicks and if they respond more to the chatter than to heterospecific nonhost calls. We tested shiny cowbird chicks reared by chalk-browed mockingbirds (Mimus saturninus) or house wrens (Troglodytes aedon) and host chicks, as control species. We randomly presented to 6-day-old chicks the following playback treatments: (1) conspecific chatter calls, (2) host calls, used as positive controls, and (3) nonhost calls, used as negative control. We measured if chicks begged during the playback treatments and the begging intensity. When responding to the playback of chatter calls, shiny cowbird chicks begged at a higher frequency and more intensively than host chicks. Shiny cowbird chicks reared by mockingbirds begged more intensively to playbacks of conspecific chatter calls than to host calls, while those reared by wrens begged with a similar intensity to playbacks of conspecific chatter and host calls. On the contrary, wren nestlings begged more intensively to playbacks of the wren call than to chatter calls. Mockingbird nestlings did not beg during any treatment. None of the three species begged during the playback of nonhost calls. Our results show that the chatter call produced a preferential begging response in cowbird nestlings, which may be the beginning of a process of conspecific recognition.

Three Dual-Frequency Precise Point Positioning Models for the Ionospheric Modeling and Satellite Pseudorange Observable-Specific Signal Bias Estimation

Global Navigation Satellite System (GNSS) Precise Point Positioning (PPP) enables the estimation the ionospheric vertical total electron content (VTEC) as well as the by-product of the satellite Pseudorange observable-specific signal bias (OSB). The single-frequency PPP models, with the ionosphere-float and ionosphere-free approaches in ionospheric studies, have recently been discussed by the authors. However, the multi-frequency observations can improve the performances of the ionospheric research compared with the single-frequency approaches. This paper presents three dual-frequency PPP approaches using the BeiDou Navigation Satellite System (BDS) B1I/B3I observations to investigate ionospheric activities. Datasets collected from the globally distributed stations are used to evaluate the performance of the ionospheric modeling with the ionospheric single- and multi-layer mapping functions (MFs), respectively. The characteristics of the estimated ionospheric VTEC and BDS satellite pseudorange OSB are both analyzed. The results indicated that the three dual-frequency PPP models could all be applied to the ionospheric studies, among which the dual-frequency ionosphere-float PPP model exhibits the best performance. The three dual-frequency PPP models all possess the capacity for ionospheric applications in the GNSS community.

An expanded palette of fluorogenic HaloTag probes with enhanced contrast for targeted cellular imaging

We report on new fluorogenic HaloTag probes based on a molecular rotor design. Thanks to their viscosity-sensitive emission, the probes light-up upon reaction with the protein self-labeling tag HaloTag. The palette of probes cover an emission range from green to red and exhibit remarkably low non-specific signal that enabled wash-free targeted imaging of intracellular organelles and proteins with good contrast in live Hela cells.

Insights into genetic factors contributing to variability in SARS-CoV-2 susceptibility and COVID-19 disease severity

Variability in SARS-CoV-2 susceptibility and COVID-19 disease severity between individuals is partly due to genetic factors. Here, we applied colocalization to compare summary statistics for 16 GWASs from the COVID-19 Host Genetics Initiative to investigate similarities and differences in their genetic signals. We identified 9 loci associated with susceptibility (one with two independent GWAS signals; one with an ethnicity-specific signal), 14 associated with severity (one with two independent GWAS signals; two with ethnicity-specific signals) and one harboring two discrepant GWAS signals (one for susceptibility; one for severity). Utilizing colocalization we also identified 45 GTEx tissues that had eQTL(s) for 18 genes strongly associated with GWAS signals in eleven loci (1-4 genes per locus). Some of these genes showed tissue-specific altered expression and others showed altered expression in up to 41 different tissue types. Our study provides insights into the complex molecular mechanisms underlying inherited predispositions to COVID-19-disease phenotypes.

A Stopping Criterion for the Training Process of the Specific Signal Generator

Mathematical description of a complex signal is very important in engineering but nearly impossible in many occasions. The emergence of the Generative Adversarial Network (GAN) shows the possibility to train a single neural network to be a Specific Signal Generator (SSG), which is only controlled by a random vector with several elements. However, there is no explicit criterion for the GAN training process to stop, and in real applications the training always stops after a certain big iteration. In this paper, a serious issue was discussed during the process to use GAN as a SSG. And, an explicit criterion for the GAN as a SSG to stop the training process were proposed. Several experiments were carried out to illustrate the issues mentioned above and the effectiveness of the stopping criterion proposed in this paper.

REFLECTION OF CHANGES IN THE TAX SYSTEM OF THE REPUBLIC OF UZBEKISTAN IN THE WORLD BANK’S «DOING BUSINESS» RATING

The author of the article noted that in recent years, with the aim of creating relaxation for business entities, the base of many normative legal acts has been regulated, measures are being taken to create a favorable business environment and a positive influence of country on the international arena. The “Doing Business” rating is a rating that reflects the level of ease of doing small and medium business in the country. In turn, this rating is an international rating, which serves as a reflection of our work in this area and a specific signal for foreign investors.

Fluorescent detection of PARP activity in unfixed tissue

Poly-ADP-ribose-polymerase (PARP) relates to a family of enzymes that can detect DNA breaks and initiate DNA repair. While this activity is generally seen as promoting cell survival, PARP enzymes are also known to be involved in cell death in numerous pathologies, including in inherited retinal degeneration. This ambiguous role of PARP makes it attractive to have a simple and fast enzyme activity assay, that allows resolving its enzymatic activity in situ, in individual cells, within complex tissues. A previously published two-step PARP activity assay uses biotinylated NAD+ and streptavidin labelling for this purpose. Here, we used the fluorescent NAD+ analogues ε-NAD+ and 6-Fluo-10-NAD+ to assess PARP activity directly on unfixed tissue sections obtained from wild-type and retinal degeneration-1 (rd1) mutant retina. In standard UV microscopy ε-NAD+ incubation did not reveal PARP specific signal. In contrast, 6-Fluo-10-NAD+ resulted in reliable detection of in situ PARP activity in rd1 retina, especially in the degenerating photoreceptor cells. When the 6-Fluo-10-NAD+ based PARP activity assay was performed in the presence of the PARP specific inhibitor olaparib, the activity signal was completely abolished, attesting to the specificity of the assay. The incubation of live organotypic retinal explant cultures with 6-Fluo-10-NAD+, did not produce PARP specific signal, indicating that the fluorescent marker may not be sufficiently membrane-permeable to label living cells. In summary, we present a new, rapid, and simple to use fluorescence assay for the cellular resolution of PARP activity on unfixed tissue, for instance in complex neuronal tissues such as the retina.