scholarly journals Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations

Cells ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 35
Author(s):  
Shane M. Hickey ◽  
Ben Ung ◽  
Christie Bader ◽  
Robert Brooks ◽  
Joanna Lazniewska ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Cellular Level ◽  
Biological Processes ◽  
Biological Models ◽  
Imaging Procedures ◽  
Imaging Tool

Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell biology, but the complexities of fluorescence microscopy as an imaging tool can sometimes be overlooked or misunderstood. This review seeks to cover the three fundamental considerations when designing fluorescence microscopy experiments: (1) hardware availability; (2) amenability of biological models to fluorescence microscopy; and (3) suitability of imaging agents for intended applications. This review will help equip the reader to make judicious decisions when designing fluorescence microscopy experiments that deliver high-resolution and informative images for cell biology.

scholarly journals Full-field supercritical angle fluorescence microscopy for live cell imaging

2011 ◽  
Vol 36 (16) ◽  
pp. 3051 ◽  
Author(s):  
Thomas Barroca ◽  
Karla Balaa ◽  
Julie Delahaye ◽  
Sandrine Lévêque-Fort ◽  
Emmanuel Fort
Keyword(s):  
Fluorescence Microscopy ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Full Field

scholarly journals Live cell imaging of macrophage/bacterium interaction demonstrates cell lysis induced by Corynebacterium diphtheriae and Corynebacterium ulcerans

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Dulanthi Weerasekera ◽  
Jonas Hahn ◽  
Martin Herrmann ◽  
Andreas Burkovski
Keyword(s):  
Fluorescence Microscopy ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Time Lapse ◽  
Corynebacterium Diphtheriae ◽  
Live Cell ◽  
Human Macrophage ◽  
Data Description ◽  
Corynebacterium Ulcerans ◽  
Microscopy Data

Abstract Objectives In frame of a study to characterize the interaction of human macrophage-like cells with pathogenic corynebacteria, Corynebacterium diphtheriae and Corynebacterium ulcerans, live cell imaging experiments were carried out and time lapse fluorescence microscopy videos were generated, which are presented here. Data description The time lapse fluorescence microscopy data revealed new insights in the interaction of corynebacteria with human macrophage-like THP-1 cells. In contrast to uninfected cells and infections with non-pathogenic C. glutamicum used as a control, pathogenic C. diphtheriae and C. ulcerans showed highly detrimental effects towards human cells and induction of cell death of macrophages.

scholarly journals A drug-compatible and temperature-controlled microfluidic device for live-cell imaging

Open Biology ◽  
2016 ◽  
Vol 6 (8) ◽  
pp. 160156 ◽  
Author(s):  
Tong Chen ◽  
Blanca Gomez-Escoda ◽  
Javier Munoz-Garcia ◽  
Julien Babic ◽  
Laurent Griscom ◽  
...  
Keyword(s):  
Cell Biology ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Critical Issue ◽  
Growth Conditions ◽  
Medium Composition ◽  
Live Cell ◽  
Model Systems ◽  
Dynamic Regulation ◽  
Cellular Environment

Monitoring cellular responses to changes in growth conditions and perturbation of targeted pathways is integral to the investigation of biological processes. However, manipulating cells and their environment during live-cell-imaging experiments still represents a major challenge. While the coupling of microfluidics with microscopy has emerged as a powerful solution to this problem, this approach remains severely underexploited. Indeed, most microdevices rely on the polymer polydimethylsiloxane (PDMS), which strongly absorbs a variety of molecules commonly used in cell biology. This effect of the microsystems on the cellular environment hampers our capacity to accurately modulate the composition of the medium and the concentration of specific compounds within the microchips, with implications for the reliability of these experiments. To overcome this critical issue, we developed new PDMS-free microdevices dedicated to live-cell imaging that show no interference with small molecules. They also integrate a module for maintaining precise sample temperature both above and below ambient as well as for rapid temperature shifts. Importantly, changes in medium composition and temperature can be efficiently achieved within the chips while recording cell behaviour by microscopy. Compatible with different model systems, our platforms provide a versatile solution for the dynamic regulation of the cellular environment during live-cell imaging.

scholarly journals From imaging to understanding: Frontiers in Live Cell Imaging, Bethesda, MD, April 19–21, 2006

2006 ◽  
Vol 174 (4) ◽  
pp. 481-484 ◽  
Author(s):  
Yu-li Wang ◽  
Klaus M. Hahn ◽  
Robert F. Murphy ◽  
Alan F. Horwitz
Keyword(s):  
Cell Biology ◽  
Live Cell Imaging ◽  
Recent Progress ◽  
Cell Imaging ◽  
Live Cell ◽  
The Other ◽  
Biological Applications ◽  
Research Fields ◽  
Recent Meeting ◽  
The One

A recent meeting entitled Frontiers in Live Cell Imaging was attended by more than 400 cell biologists, physicists, chemists, mathematicians, and engineers. Unlike typical special topics meetings, which bring together investigators in a defined field primarily to review recent progress, the purpose of this meeting was to promote cross-disciplinary interactions by introducing emerging methods on the one hand and important biological applications on the other. The goal was to turn live cell imaging from a “technique” used in cell biology into a new exploratory science that combines a number of research fields.

Fast microfluidic temperature control for high resolution live cell imaging

Lab on a Chip ◽  
2011 ◽  
Vol 11 (3) ◽  
pp. 484-489 ◽  
Author(s):  
Guilhem Velve Casquillas ◽  
Chuanhai Fu ◽  
Mael Le Berre ◽  
Jeremy Cramer ◽  
Sebastien Meance ◽  
...  
Keyword(s):  
High Resolution ◽  
Temperature Control ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell
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